Question 1

Most light microscopes contain a/an ________ that converges the light beam so that it passes through the specimen.

Accepted Answer

A) objective lens 
B) iris diaphragm 
C) mechanical stage 
D) condenser 
A) objective lens 
B) iris diaphragm 
C) mechanical stage 
D) condenser

Question 2

A microscope that converts changes in the speed of light as it passes through an object into differences in brightness is a ________ microscope.

Accepted Answer

A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski 
A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski

Question 3

Electron microscopes use ________ to focus the electron beam.

Accepted Answer

A) glass lenses 
B) electromagnets 
C) mechanical stages 
D) laser beams 
A) glass lenses 
B) electromagnets 
C) mechanical stages 
D) laser beams

Question 4

A compound light microscope can generally see objects as no smaller than a _____.

Accepted Answer

A) ribosome 
B) large protozoa 
C) small bacterium 
D) typical virus 
A) ribosome 
B) large protozoa 
C) small bacterium 
D) typical virus

Question 5

The term "basic dyes" refers to the fact that these dyes are _____.

Accepted Answer

A) easily prepared 
B) positively charged 
C) attracted to positively charged cell structures 
D) simple in their composition 
A) easily prepared 
B) positively charged 
C) attracted to positively charged cell structures 
D) simple in their composition

Question 6

A compound microscope has _____.

Accepted Answer

A) two eyepieces 
B) a total magnification of 5,000X 
C) only fine adjustment and no coarse adjustment 
D) more than one lens 
A) two eyepieces 
B) a total magnification of 5,000X 
C) only fine adjustment and no coarse adjustment 
D) more than one lens

Question 7

Ultraviolet light is a key component of:

Accepted Answer

A) bright-field microscopy 
B) dark-field microscopy 
C) phase-contrast microscopy 
D) fluorescence microscopy 
A) bright-field microscopy 
B) dark-field microscopy 
C) phase-contrast microscopy 
D) fluorescence microscopy

Question 8

To calculate the total magnification of a light microscope you must know the magnification of the _____ lenses.

Accepted Answer

A) objective and condenser 
B) ocular and condenser 
C) objective and condenser. 
D) objective and ocular. 
A) objective and condenser 
B) ocular and condenser 
C) objective and condenser. 
D) objective and ocular.

Question 9

Light of ________ wavelength typically will result in ________ resolution.

Accepted Answer

A) longer, better 
B) shorter, better 
C) any, poor 
D) shorter, worse 
A) longer, better 
B) shorter, better 
C) any, poor 
D) shorter, worse

Question 10

Which of the following statements about Leeuwenhoek's microscopes is false?

Accepted Answer

A) Leeuwenhoek kept his technique secret. 
B) They magnified objects 100 to 300 times. 
C) For each specimen a new microscope had to be made. 
D) They were able to reveal very fine details of bacteria. 
A) Leeuwenhoek kept his technique secret. 
B) They magnified objects 100 to 300 times. 
C) For each specimen a new microscope had to be made. 
D) They were able to reveal very fine details of bacteria.

Question 11

Diffraction occurs when light _____.

Accepted Answer

A) is reflected by an object 
B) passes through a small opening 
C) changes wavelengths 
D) is absorbed by a normally transparent object (like a glass slide) 
A) is reflected by an object 
B) passes through a small opening 
C) changes wavelengths 
D) is absorbed by a normally transparent object (like a glass slide)

Question 12

Three dimensional views of cells and other small objects could best be obtained using a:

Accepted Answer

A) phase contrast microscope 
B) dark-field microscope 
C) transmission electron microscope (TEM) 
D) scanning electron microscope (SEM) 
A) phase contrast microscope 
B) dark-field microscope 
C) transmission electron microscope (TEM) 
D) scanning electron microscope (SEM)

Question 13

Colored photos of electron micrographs:

Accepted Answer

A) reflect the color of the specimen before it was frozen 
B) are false color added on during image preparation 
C) reflect the color of the specimen after it was frozen 
D) are always in pastel shades 
A) reflect the color of the specimen before it was frozen 
B) are false color added on during image preparation 
C) reflect the color of the specimen after it was frozen 
D) are always in pastel shades

Question 14

Why do basic dyes attach to most bacterial surfaces?

Accepted Answer

A) Most bacterial surfaces are negatively charged. 
B) Bacterial cells take up safranin. 
C) Most bacterial surfaces resist taking up the stain. 
D) Most bacterial surfaces do not have a charge. 
A) Most bacterial surfaces are negatively charged. 
B) Bacterial cells take up safranin. 
C) Most bacterial surfaces resist taking up the stain. 
D) Most bacterial surfaces do not have a charge.

Question 15

Electron microscopes have a much better resolving power when compared to light microscopes because electrons _____.

Accepted Answer

A) are invisible to the eye 
B) have longer wavelengths than visible light rays 
C) have shorter wavelengths than visible light rays 
D) are negatively charged 
A) are invisible to the eye 
B) have longer wavelengths than visible light rays 
C) have shorter wavelengths than visible light rays 
D) are negatively charged

Question 16

In a Gram stain,the mordant is _____.

Accepted Answer

A) crystal violet 
B) iodine 
C) water 
D) alcohol 
A) crystal violet 
B) iodine 
C) water 
D) alcohol

Question 17

The formula for the resolving power (resolution distance)of a lens is ƛ/2NA (wavelength /2 x numerical aperture). What does this say about resolving power?

Accepted Answer

A) The smaller the wavelength, the greater the resolving power of the lens. 
B) Resolving power is not related to the lens' numerical aperture. 
C) We cannot precisely calculate a lens' resolving power. 
D) A larger resolving power is indicative of a better lens. 
A) The smaller the wavelength, the greater the resolving power of the lens. 
B) Resolving power is not related to the lens' numerical aperture. 
C) We cannot precisely calculate a lens' resolving power. 
D) A larger resolving power is indicative of a better lens.

Question 18

The image of this fungus was taken using a:

Accepted Answer

A) confocal microscope 
B) atomic force microscope 
C) compound light microscope 
D) fluorescence microscope 
A) confocal microscope 
B) atomic force microscope 
C) compound light microscope 
D) fluorescence microscope

Question 19

In a properly executed Gram stain,Gram positive organisms appear ________ while Gram negative organisms appear ________.

Accepted Answer

A) pink, clear 
B) pink, purple 
C) purple, pink 
D) purple, blue 
A) pink, clear 
B) pink, purple 
C) purple, pink 
D) purple, blue

Question 20

Which of the following can be used to examine live specimens?

Accepted Answer

A) TEM 
B) SEM 
C) scanning tunneling electron microscope 
D) atomic force microscope 
A) TEM 
B) SEM 
C) scanning tunneling electron microscope 
D) atomic force microscope

Exam 3: Microscopy and Staining

Most light microscopes contain a/an ________ that converges the light beam so that it passes through the specimen.

A microscope that converts changes in the speed of light as it passes through an object into differences in brightness is a ________ microscope.

Electron microscopes use ________ to focus the electron beam.

A compound light microscope can generally see objects as no smaller than a _____.

The term "basic dyes" refers to the fact that these dyes are _____.

A compound microscope has _____.

Ultraviolet light is a key component of:

To calculate the total magnification of a light microscope you must know the magnification of the _____ lenses.

Light of ________ wavelength typically will result in ________ resolution.

Which of the following statements about Leeuwenhoek's microscopes is false?

Diffraction occurs when light _____.

Three dimensional views of cells and other small objects could best be obtained using a:

Colored photos of electron micrographs:

Why do basic dyes attach to most bacterial surfaces?

Electron microscopes have a much better resolving power when compared to light microscopes because electrons _____.

In a Gram stain,the mordant is _____.

The formula for the resolving power (resolution distance)of a lens is ƛ/2NA (wavelength /2 x numerical aperture). What does this say about resolving power?

The image of this fungus was taken using a:

In a properly executed Gram stain,Gram positive organisms appear ________ while Gram negative organisms appear ________.

Which of the following can be used to examine live specimens?

Scope and History of Microbiology

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunology Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

Light of wavelength typically will result in resolution.

In a properly executed Gram stain,Gram positive organisms appear while Gram negative organisms appear .