Question 1

Before performing a phlebotomy,the phlebotomist will clean the area on a patient's arm with a substance before inserting the needle.This substance is called a(n):

Accepted Answer

A)  Disinfectant 
B)  Sterilizer 
C)  Antiseptic 
D)  Bactericidal 
A)  Disinfectant 
B)  Sterilizer 
C)  Antiseptic 
D)  Bactericidal C

Question 2

All of the following are examples of personal protective equipment (PPE)EXCEPT:

Accepted Answer

A)  Gloves 
B)  Lab coats 
C)  Safety glasses 
D)  Prescription glasses 
A)  Gloves 
B)  Lab coats 
C)  Safety glasses 
D)  Prescription glasses D

Question 3

Routine handwashing in health care settings mandates washing at all the following times EXCEPT:

Accepted Answer

A)  In high-risk areas such as ICU and burn units 
B)  On entering protective isolation units 
C)  Before and after routine patient contact 
D)  When gloves become soiled during a procedure or dressing change on the same patient. 
A)  In high-risk areas such as ICU and burn units 
B)  On entering protective isolation units 
C)  Before and after routine patient contact 
D)  When gloves become soiled during a procedure or dressing change on the same patient. D

Question 4

The chemical or physical method that destroys all forms of life is called:

Accepted Answer

A)  Sterilization 
B)  Disinfection 
C)  Bacteriostatic 
D)  Bactericidal 
A)  Sterilization 
B)  Disinfection 
C)  Bacteriostatic 
D)  Bactericidal

Question 5

Because Biosafety Level 3 (BSL-3)organisms have the potential for aerosol transmission,and diseases with these agents may have serious lethal consequences,all of the following guidelines apply to BSL-3 labs EXCEPT:

Accepted Answer

A)  BSL-3 labs should be separated from other parts of the building by an anteroom. 
B)  BSL-3 labs should have restricted access with logbook documentation of personnel entering and exiting lab. 
C)  BSL-3 labs should have solid ceiling and floor seams, and any seams must be sealed. 
D)  BSL-3 labs should have positive air pressure. 
A)  BSL-3 labs should be separated from other parts of the building by an anteroom. 
B)  BSL-3 labs should have restricted access with logbook documentation of personnel entering and exiting lab. 
C)  BSL-3 labs should have solid ceiling and floor seams, and any seams must be sealed. 
D)  BSL-3 labs should have positive air pressure.

Question 6

Universal/Standard Precautions require that:

Accepted Answer

A)  Only some body fluids be considered infectious and capable of transmitting disease 
B)  Body fluids with visible blood be treated as noninfectious 
C)  Blood and body fluids from all patients be considered infectious and capable of transmitting disease 
D)  Urine and feces be considered noninfectious 
A)  Only some body fluids be considered infectious and capable of transmitting disease 
B)  Body fluids with visible blood be treated as noninfectious 
C)  Blood and body fluids from all patients be considered infectious and capable of transmitting disease 
D)  Urine and feces be considered noninfectious

Question 7

The tech needs to sterilize a piece of equipment that cannot be autoclaved or gas sterilized because the equipment contains lenses,metal,and rubber components.What solution should be used to sterilize this piece of equipment?

Accepted Answer

A)  10% bleach 
B)  37% formalin 
C)  Glutaraldehyde 
D)  90% alcohol 
A)  10% bleach 
B)  37% formalin 
C)  Glutaraldehyde 
D)  90% alcohol

Question 8

Biosafety Level 4 (BSL-4)organisms are dangerous,exotic,and pose an increased risk of aerosol-transmitted infections and life-threatening disease.Two examples of BSL-4 organisms are:

Accepted Answer

A)  Smallpox and Ebola 
B)  St. Louis encephalitis virus and Coccidioides immitis 
C)  Mycobacterium tuberculosis and Ebola 
D)  Francisella tularensis and Ebola 
A)  Smallpox and Ebola 
B)  St. Louis encephalitis virus and Coccidioides immitis 
C)  Mycobacterium tuberculosis and Ebola 
D)  Francisella tularensis and Ebola

Question 9

The disinfectant label should indicate several highlighted points important in selecting the appropriate agents for designated use,including all the following EXCEPT:

Accepted Answer

A)  Level of biocidal action 
B)  Alcohol content 
C)  The nature of microbial contamination 
D)  Structure and design of an item to be disinfected 
A)  Level of biocidal action 
B)  Alcohol content 
C)  The nature of microbial contamination 
D)  Structure and design of an item to be disinfected

Question 10

The comprehensive safety program for the microbiology laboratory needs to fulfill all the following provision EXCEPT:

Accepted Answer

A)  It is specific to the hospital and does not need to conform to state, local, and federal regulations. 
B)  It must address biologic hazards. 
C)  It must teach correct techniques for lifting and moving heavy objects and patients (where applicable). 
D)  It must describe the safe handling, storage, and disposal of chemicals. 
A)  It is specific to the hospital and does not need to conform to state, local, and federal regulations. 
B)  It must address biologic hazards. 
C)  It must teach correct techniques for lifting and moving heavy objects and patients (where applicable). 
D)  It must describe the safe handling, storage, and disposal of chemicals.

Question 11

Most institutions use the RACE acronym to respond to a fire emergency.RACE stands for

Accepted Answer

A)  Race, Alert, Cite, Evacuate 
B)  Run, Avoid, Call, Emergency 
C)  Rescue, Alarm, Contain, Extinguish 
D)  Retrieve, Announce, Close, Exit 
A)  Race, Alert, Cite, Evacuate 
B)  Run, Avoid, Call, Emergency 
C)  Rescue, Alarm, Contain, Extinguish 
D)  Retrieve, Announce, Close, Exit

Question 12

Processing of patient specimens and handling of actively growing cultures of microorganisms puts an employee at risk of potential contact with the infectious agent through all the following routes EXCEPT:

Accepted Answer

A)  Mucous membranes 
B)  Blood splashed onto intact skin 
C)  Inhalation of aerosols 
D)  Accidental ingestion 
A)  Mucous membranes 
B)  Blood splashed onto intact skin 
C)  Inhalation of aerosols 
D)  Accidental ingestion

Question 13

A laboratory technician is working with tissue infected with Mycobacterium tuberculosis that has been preserved in formalin for a couple years.After dissecting the tissue,how should the worker sterilize the work area?

Accepted Answer

A)  Wipe down the work area with a 37% formalin solution. 
B)  Wipe down the work area with a 10% bleach solution. 
C)  Wipe down the work area with a 50% alcohol solution. 
D)  Use formaldehyde gas to clean the area. 
A)  Wipe down the work area with a 37% formalin solution. 
B)  Wipe down the work area with a 10% bleach solution. 
C)  Wipe down the work area with a 50% alcohol solution. 
D)  Use formaldehyde gas to clean the area.

Question 14

When eliminating organisms from inanimate objects,higher numbers of organisms require longer exposure times because:

Accepted Answer

A)  All disinfecting agents are not alike and some require shorter times. 
B)  The chemical composition of the disinfecting agent varies. 
C)  Disinfecting agents containing carbon tetrachloride require longer times to act. 
D)  It takes longer to eliminate 99% of microorganisms. 
A)  All disinfecting agents are not alike and some require shorter times. 
B)  The chemical composition of the disinfecting agent varies. 
C)  Disinfecting agents containing carbon tetrachloride require longer times to act. 
D)  It takes longer to eliminate 99% of microorganisms.

Question 15

Disinfectants containing heavy metal compounds have been replaced as disinfectants,except for _____,which is still used as a prophylactic treatment in newborns to prevent gonococcal conjunctivitis.

Accepted Answer

A)  Silver nitrate 
B)  Copper sulfate 
C)  Ammonium nitrate 
D)  Mercuric chloride 
A)  Silver nitrate 
B)  Copper sulfate 
C)  Ammonium nitrate 
D)  Mercuric chloride

Question 16

The laboratory exposure control plan should contain all the following EXCEPT:

Accepted Answer

A)  Engineer and work practice controls 
B)  Review of control plan every 5 years 
C)  Methods of compliance for Standard Precautions 
D)  Guidelines for handling and disposal of regulated waste 
A)  Engineer and work practice controls 
B)  Review of control plan every 5 years 
C)  Methods of compliance for Standard Precautions 
D)  Guidelines for handling and disposal of regulated waste

Question 17

Why is laboratory-acquired infection an obvious hazard for personnel working in a microbiology lab?

Accepted Answer

A)  Microbiology personnel do not always adhere to safety practices. 
B)  Safety practices are not applicable for the microbiology laboratory. 
C)  In-service education is not provided for microbiology staff. 
D)  Personnel deal with a variety of infections agents: viral, fungal, parasitic, and bacterial. 
A)  Microbiology personnel do not always adhere to safety practices. 
B)  Safety practices are not applicable for the microbiology laboratory. 
C)  In-service education is not provided for microbiology staff. 
D)  Personnel deal with a variety of infections agents: viral, fungal, parasitic, and bacterial.

Question 18

Three levels of laboratories outlined in the Laboratory Response Network (LRN)include all the following EXCEPT:

Accepted Answer

A)  Sentinel 
B)  Reference 
C)  First responder 
D)  National responder 
A)  Sentinel 
B)  Reference 
C)  First responder 
D)  National responder

Question 19

If a laboratory uses hazardous chemicals,it must:

Accepted Answer

A)  Keep a chemical inventory and Material Safety Data Sheets (MSDSs) on hand. 
B)  Keep chemicals in a special corrosive cabinet. 
C)  Keep chemicals in a special locking cabinet. 
D)  Post chemical names and locations on the door of the laboratory. 
A)  Keep a chemical inventory and Material Safety Data Sheets (MSDSs) on hand. 
B)  Keep chemicals in a special corrosive cabinet. 
C)  Keep chemicals in a special locking cabinet. 
D)  Post chemical names and locations on the door of the laboratory.

Question 20

Disinfectants are usually used at this temperature.

Accepted Answer

A)  50° to 100° C 
B)  0° to 10° C 
C)  25° to 50° C 
D)  20° to 22° C 
A)  50° to 100° C 
B)  0° to 10° C 
C)  25° to 50° C 
D)  20° to 22° C

Exam 4: Control of Microorganisms

Before performing a phlebotomy,the phlebotomist will clean the area on a patient's arm with a substance before inserting the needle.This substance is called a(n):

All of the following are examples of personal protective equipment (PPE)EXCEPT:

Routine handwashing in health care settings mandates washing at all the following times EXCEPT:

The chemical or physical method that destroys all forms of life is called:

Because Biosafety Level 3 (BSL-3)organisms have the potential for aerosol transmission,and diseases with these agents may have serious lethal consequences,all of the following guidelines apply to BSL-3 labs EXCEPT:

Universal/Standard Precautions require that:

The tech needs to sterilize a piece of equipment that cannot be autoclaved or gas sterilized because the equipment contains lenses,metal,and rubber components.What solution should be used to sterilize this piece of equipment?

Biosafety Level 4 (BSL-4)organisms are dangerous,exotic,and pose an increased risk of aerosol-transmitted infections and life-threatening disease.Two examples of BSL-4 organisms are:

The disinfectant label should indicate several highlighted points important in selecting the appropriate agents for designated use,including all the following EXCEPT:

The comprehensive safety program for the microbiology laboratory needs to fulfill all the following provision EXCEPT:

Most institutions use the RACE acronym to respond to a fire emergency.RACE stands for

Processing of patient specimens and handling of actively growing cultures of microorganisms puts an employee at risk of potential contact with the infectious agent through all the following routes EXCEPT:

A laboratory technician is working with tissue infected with Mycobacterium tuberculosis that has been preserved in formalin for a couple years.After dissecting the tissue,how should the worker sterilize the work area?

When eliminating organisms from inanimate objects,higher numbers of organisms require longer exposure times because:

Disinfectants containing heavy metal compounds have been replaced as disinfectants,except for _____,which is still used as a prophylactic treatment in newborns to prevent gonococcal conjunctivitis.

The laboratory exposure control plan should contain all the following EXCEPT:

Why is laboratory-acquired infection an obvious hazard for personnel working in a microbiology lab?

Three levels of laboratories outlined in the Laboratory Response Network (LRN)include all the following EXCEPT:

If a laboratory uses hazardous chemicals,it must:

Disinfectants are usually used at this temperature.

Bacterial Cell Structure, physiology, Metabolism, and Genetics

Host-Parasite Interaction

Laboratory Role in Infection Control

Performance Improvement in the Microbiology Laboratory

Specimen Collection and Processing

Microscopic Examination of Infected Materials

Use of Colonial Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

Applications of Molecular Diagnostics

Antibiotic Mechanisms of Action and Resistance

Antimicrobial Susceptibility Testing

Staphylococci

Streptococcus, enterococcus, and Other Catalase-Negative Gram-Positive Cocci

Aerobic Gram-Positive Bacilli

Neisseria Species and Moraxella Catarrhalis

Haemophilus and Other Fastidious Gram-Negative Bacilli

Enterobacteriaceae

Vibrio,aeromonas,plesiomonas and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

Anaerobes of Clinical Importance

Spirochetes

Chlamydia and Rickettsia

Mycoplasma and Ureaplasma

Mycobacterium Tuberculosis and Other Nontuberculosis Mycobacteria

Medically Significant Fungi

Diagnostic Parasitology

Clinical Virology

Agents of Bioterror

Biofilms: Architects of Disease

Upper and Lower Respiratory Tract Infections

Skin and Soft Tissue Infections

Gastrointestinal Infections and Food Poisoning

Infections of the Central Nervous System

Bacteremia and Sepsis

Urinary Tract Infections

Genital Infections and Sexually Transmitted Diseases

Infections in Special Populations

Zoonotic Diseases

Ocular Infections

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