Question 1

What are the sugars present in triple sugar iron (TSI)?

Accepted Answer

A)  Sucrose, glucose, lactose 
B)  Mannitol, lactose, arabinose 
C)  Sucrose, glucose, rhamnose 
D)  Lactose, sucrose, raffinose 
A)  Sucrose, glucose, lactose 
B)  Mannitol, lactose, arabinose 
C)  Sucrose, glucose, rhamnose 
D)  Lactose, sucrose, raffinose A

Question 2

What medium is used in the urease test?

Accepted Answer

A)  Christensen's media 
B)  Loeffler's media 
C)  Möeller's oxidative broth 
D)  Kovac's reagent 
A)  Christensen's media 
B)  Loeffler's media 
C)  Möeller's oxidative broth 
D)  Kovac's reagent A

Question 3

How are organisms identified with commercial identification kits?

Accepted Answer

A)  Numeric codes 
B)  Color codes 
C)  Alphabetic codes 
D)  Alphanumeric codes 
A)  Numeric codes 
B)  Color codes 
C)  Alphabetic codes 
D)  Alphanumeric codes A

Question 4

If a bacterium utilizes lactose or sucrose,what will the triple sugar iron (TSI)reaction look like?

Accepted Answer

A)  Alkaline/acid 
B)  Alkaline/alkaline 
C)  Acid/acid 
D)  Acid/alkaline 
A)  Alkaline/acid 
B)  Alkaline/alkaline 
C)  Acid/acid 
D)  Acid/alkaline

Question 5

To inoculate triple sugar iron (TSI)agar or Kligler's iron agar (KIA),the laboratory scientist should pick a well-isolated colony with an inoculating needle and:

Accepted Answer

A)  Touch the bottom of the needle to the bottom of the slant portion of the agar and move the needle back and forth across the surface of the slant all the way to the top of the tube. 
B)  Touch the bottom of the needle to the top of the slant portion of the agar and move the needle back and forth across the surface of the slant all the way to the bottom of the tube. 
C)  Touch the bottom of the needle to the bottom of the slant portion of the agar and move the needle up the slant in a straight line all the way to the top of the tube. 
D)  Stab the butt almost all the way to the bottom of the tube, then move the needle back and forth over the surface of the slant all the way to the top of the tube. 
A)  Touch the bottom of the needle to the bottom of the slant portion of the agar and move the needle back and forth across the surface of the slant all the way to the top of the tube. 
B)  Touch the bottom of the needle to the top of the slant portion of the agar and move the needle back and forth across the surface of the slant all the way to the bottom of the tube. 
C)  Touch the bottom of the needle to the bottom of the slant portion of the agar and move the needle up the slant in a straight line all the way to the top of the tube. 
D)  Stab the butt almost all the way to the bottom of the tube, then move the needle back and forth over the surface of the slant all the way to the top of the tube.

Question 6

What amino acid is the substrate in indole test broth?

Accepted Answer

A)  Ornithine 
B)  Arginine 
C)  Tryptophan 
D)  Leucine 
A)  Ornithine 
B)  Arginine 
C)  Tryptophan 
D)  Leucine

Question 7

What test determines whether an organism can utilize sodium citrate as a sole carbon source?

Accepted Answer

A)  Ornithine decarboxylase 
B)  Citrate 
C)  Indole 
D)  Ehrlich's reagent 
A)  Ornithine decarboxylase 
B)  Citrate 
C)  Indole 
D)  Ehrlich's reagent

Question 8

Other sugars that bacteria can use to produce energy for glucose include all of the following EXCEPT:

Accepted Answer

A)  Raffinose 
B)  Arabinose 
C)  Rhamnose 
D)  Sucralose 
A)  Raffinose 
B)  Arabinose 
C)  Rhamnose 
D)  Sucralose

Question 9

The lysine iron agar (LIA)slant is also useful in differentiating:

Accepted Answer

A)  Acinetobacter and Alcaligenes 
B)  Klebsiella and Enterobacteriaceae 
C)  Proteus, Morganella, and Providencia 
D)  Escherichia, Salmonella, and Campylobacter 
A)  Acinetobacter and Alcaligenes 
B)  Klebsiella and Enterobacteriaceae 
C)  Proteus, Morganella, and Providencia 
D)  Escherichia, Salmonella, and Campylobacter

Question 10

What does a lysine iron agar (LIA)slant contain?

Accepted Answer

A)  Ornithine, glucose, ferric ammonium chloride, and urea 
B)  Lysine, glucose, ferric ammonium citrate, and sodium thiosulfate 
C)  Acetoin, pyruvic acid, nitrate, and sulfanilic acid 
D)  Arginine, lactose, ferric chloride, sodium hydroxide, and urea 
A)  Ornithine, glucose, ferric ammonium chloride, and urea 
B)  Lysine, glucose, ferric ammonium citrate, and sodium thiosulfate 
C)  Acetoin, pyruvic acid, nitrate, and sulfanilic acid 
D)  Arginine, lactose, ferric chloride, sodium hydroxide, and urea

Question 11

During this process,glucose enters the glycolysis pathway,resulting in the formation of pyruvic acid,which is further oxidized to other acids.What is this process called?

Accepted Answer

A)  Oxidation 
B)  Reduction 
C)  Fermentation 
D)  Synthesis 
A)  Oxidation 
B)  Reduction 
C)  Fermentation 
D)  Synthesis

Question 12

Oxidative/fermentative (O/F)basal medium is a medium that will test the oxidative and fermentative capabilities of a microbe.The pH indicator is _____.

Accepted Answer

A)  Sudan IV 
B)  Phenolphthalein 
C)  Bromcresol green 
D)  Bromthymol blue 
A)  Sudan IV 
B)  Phenolphthalein 
C)  Bromcresol green 
D)  Bromthymol blue

Question 13

The principle of the citrate test includes all the following EXCEPT:

Accepted Answer

A)  Bacteria able to use citrate will use the ammonium salts releasing ammonia. 
B)  The alkaline pH that results from use of the ammonium salts changes the pH indicator. 
C)  The pH indicator changes from red to bright yellow. 
D)  The pH indicator changes from green to blue. 
A)  Bacteria able to use citrate will use the ammonium salts releasing ammonia. 
B)  The alkaline pH that results from use of the ammonium salts changes the pH indicator. 
C)  The pH indicator changes from red to bright yellow. 
D)  The pH indicator changes from green to blue.

Question 14

What chemicals are added to triple sugar iron (TSI)to detect the production of hydrogen sulfide gas?

Accepted Answer

A)  Phenol red and ferrous sulfate 
B)  Bromthymol blue and sodium thiosulfate 
C)  Ferrous sulfate and sodium thiosulfate 
D)  Copper sulfate and ferrous sulfate 
A)  Phenol red and ferrous sulfate 
B)  Bromthymol blue and sodium thiosulfate 
C)  Ferrous sulfate and sodium thiosulfate 
D)  Copper sulfate and ferrous sulfate

Question 15

The initial screening of gram-negative rods is done by testing for the utilization of this carbohydrate.

Accepted Answer

A)  Glucose 
B)  Sucrose 
C)  Lactose 
D)  Mannitol 
A)  Glucose 
B)  Sucrose 
C)  Lactose 
D)  Mannitol

Question 16

In the Voges-Proskauer test,you are testing for the metabolic by-products of which pathway?

Accepted Answer

A)  Glucose ® formic acid ® formaldehyde 
B)  Glucose ® pyruvic acid ® mixed acid fermentation 
C)  Lactose ® glucose ® 2,3 butanediol 
D)  Glucose ® pyruvic acid ® acetoin ® diacetyl KOH 
A)  Glucose ® formic acid ® formaldehyde 
B)  Glucose ® pyruvic acid ® mixed acid fermentation 
C)  Lactose ® glucose ® 2,3 butanediol 
D)  Glucose ® pyruvic acid ® acetoin ® diacetyl KOH

Question 17

These bacteria are unable to ferment either lactose or glucose,but they can degrade the peptones present in the triple sugar iron (TSI)agar or Kligler's iron agar (KIA)aerobically or anaerobically,resulting in the production of alkaline by-products in the slant or deep,respectively,changing the indicator to a deep red color.What kind of bacteria cannot ferment lactose or glucose?

Accepted Answer

A)  Nonenteric bacilli 
B)  Anaerobic bacilli 
C)  Gram-positive cocci 
D)  Gram-positive rods 
A)  Nonenteric bacilli 
B)  Anaerobic bacilli 
C)  Gram-positive cocci 
D)  Gram-positive rods

Question 18

Some bacteria produce these enzymes that break down gelatin into amino acids.These enzymes are called:

Accepted Answer

A)  DNAase 
B)  Gelatinase 
C)  Proteolytic 
D)  Malonase 
A)  DNAase 
B)  Gelatinase 
C)  Proteolytic 
D)  Malonase

Question 19

The chemical reaction for the Ortho-Nitrophenyl-b-D-galactopyranoside (ONPG)test is:

Accepted Answer

A)  b-galactosidase hydrolyzes lactose into glucose and galactose 
B)  Glucose ® pyruvic acid ® acetoin 
C)  b-Galactosidase hydrolyzes o-nitrophenyl-b-D-galactopyranoside into galactose and o-nitrophenol (a yellow compound) 
D)  O-nitrophenyl-b-D-galactopyranoside is oxidized into acetoin and glucose 
A)  b-galactosidase hydrolyzes lactose into glucose and galactose 
B)  Glucose ® pyruvic acid ® acetoin 
C)  b-Galactosidase hydrolyzes o-nitrophenyl-b-D-galactopyranoside into galactose and o-nitrophenol (a yellow compound) 
D)  O-nitrophenyl-b-D-galactopyranoside is oxidized into acetoin and glucose

Question 20

The two reagents used in the Voges-Proskauer Test are:

Accepted Answer

A)  Methyl red and acetoin 
B)  40% KOH and methyl red 
C)  o-Nitrophenol and a-naphthol 
D)  40% KOH and a-naphthol 
A)  Methyl red and acetoin 
B)  40% KOH and methyl red 
C)  o-Nitrophenol and a-naphthol 
D)  40% KOH and a-naphthol

Exam 9: Biochemical Identification of Gram-Negative Bacteria

What are the sugars present in triple sugar iron (TSI)?

What medium is used in the urease test?

How are organisms identified with commercial identification kits?

If a bacterium utilizes lactose or sucrose,what will the triple sugar iron (TSI)reaction look like?

To inoculate triple sugar iron (TSI)agar or Kligler's iron agar (KIA),the laboratory scientist should pick a well-isolated colony with an inoculating needle and:

What amino acid is the substrate in indole test broth?

What test determines whether an organism can utilize sodium citrate as a sole carbon source?

Other sugars that bacteria can use to produce energy for glucose include all of the following EXCEPT:

The lysine iron agar (LIA)slant is also useful in differentiating:

What does a lysine iron agar (LIA)slant contain?

During this process,glucose enters the glycolysis pathway,resulting in the formation of pyruvic acid,which is further oxidized to other acids.What is this process called?

Oxidative/fermentative (O/F)basal medium is a medium that will test the oxidative and fermentative capabilities of a microbe.The pH indicator is _____.

The principle of the citrate test includes all the following EXCEPT:

What chemicals are added to triple sugar iron (TSI)to detect the production of hydrogen sulfide gas?

The initial screening of gram-negative rods is done by testing for the utilization of this carbohydrate.

In the Voges-Proskauer test,you are testing for the metabolic by-products of which pathway?

Some bacteria produce these enzymes that break down gelatin into amino acids.These enzymes are called:

The chemical reaction for the Ortho-Nitrophenyl-b-D-galactopyranoside (ONPG)test is:

The two reagents used in the Voges-Proskauer Test are:

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