Question 1

If a test is not considered appropriate for the specimen,the following should happen:

Accepted Answer

A)  Discipline the ward clerk for ordering the wrong test. 
B)  Discipline the nurse for ordering the wrong test. 
C)  Hold a training session to teach hospital staff about correctly ordering tests. 
D)  The laboratory needs to communicate with the physician to determine exactly what needs to be done. 
A)  Discipline the ward clerk for ordering the wrong test. 
B)  Discipline the nurse for ordering the wrong test. 
C)  Hold a training session to teach hospital staff about correctly ordering tests. 
D)  The laboratory needs to communicate with the physician to determine exactly what needs to be done.

Question 2

The specimen of choice for detecting gastrointestinal pathogens is:

Accepted Answer

A)  A urine specimen 
B)  Vomit 
C)  A swab of the anal area with no feces on it 
D)  A stool specimen 
A)  A urine specimen 
B)  Vomit 
C)  A swab of the anal area with no feces on it 
D)  A stool specimen

Question 3

All specimens must be transported:

Accepted Answer

A)  In leak-proof secondary containers 
B)  In syringes with needles attached 
C)  In a tube of broth 
D)  In a latex glove 
A)  In leak-proof secondary containers 
B)  In syringes with needles attached 
C)  In a tube of broth 
D)  In a latex glove

Question 4

Why is a clean-catch midstream urine used for a urine culture as opposed to a clean-catch urine?

Accepted Answer

A)  The first portion of the urine flow washes contaminants from the urethra and the next portion of urine is more representative of the bladder. 
B)  The name was changed, but the procedure remains the same and the entire amount of urine from the bladder is cultured. 
C)  No urine is free from contamination, so just wipe the external genitalia and void into the cup. 
D)  Catheterized specimens are also called clean-catch midstream urine specimen. 
A)  The first portion of the urine flow washes contaminants from the urethra and the next portion of urine is more representative of the bladder. 
B)  The name was changed, but the procedure remains the same and the entire amount of urine from the bladder is cultured. 
C)  No urine is free from contamination, so just wipe the external genitalia and void into the cup. 
D)  Catheterized specimens are also called clean-catch midstream urine specimen.

Question 5

To perform a quality laboratory analysis,the laboratory needs specific information regarding the patient and the specimen.What can be a critical weak link in the specimen management process?

Accepted Answer

A)  Poor specimen collection techniques 
B)  Incomplete information on the requisition 
C)  Poor-quality hospital information system 
D)  Poor-quality laboratory information system 
A)  Poor specimen collection techniques 
B)  Incomplete information on the requisition 
C)  Poor-quality hospital information system 
D)  Poor-quality laboratory information system

Question 6

Two types of specimens can use preservatives to maintain them until they can be delivered to the laboratory.They are:

Accepted Answer

A)  Urine, stool 
B)  Urine, vaginal secretions 
C)  Stool, throat cultures 
D)  Pus from a wound, vaginal secretions 
A)  Urine, stool 
B)  Urine, vaginal secretions 
C)  Stool, throat cultures 
D)  Pus from a wound, vaginal secretions

Question 7

What method is used to concentrate specimens to increase recovery of pathogens in the microbiology laboratory?

Accepted Answer

A)  Homogenization 
B)  Centrifugation 
C)  Mixing 
D)  Anticoagulation 
A)  Homogenization 
B)  Centrifugation 
C)  Mixing 
D)  Anticoagulation

Question 8

How is culture media selected for specimen plating?

Accepted Answer

A)  Determine the supplemental agar needed as well as the broth media. 
B)  Always include nonselective media and differential media to help save a day of reading the culture on the bench. 
C)  Use only broth for the initial inoculation and subculture to plates after 24 hours of incubation. 
D)  The selection of media to inoculate is based on the type of specimen submitted for culture and the organisms likely to be involved in the infectious process. 
A)  Determine the supplemental agar needed as well as the broth media. 
B)  Always include nonselective media and differential media to help save a day of reading the culture on the bench. 
C)  Use only broth for the initial inoculation and subculture to plates after 24 hours of incubation. 
D)  The selection of media to inoculate is based on the type of specimen submitted for culture and the organisms likely to be involved in the infectious process.

Question 9

Nonroutine specimens can include all the following specimen types EXCEPT:

Accepted Answer

A)  Vein grafts 
B)  Syringe needles 
C)  Water samples 
D)  Multiple-lumen catheters 
A)  Vein grafts 
B)  Syringe needles 
C)  Water samples 
D)  Multiple-lumen catheters

Question 10

All the following principles of specimen collection are fundamental to ensuring appropriate specimen management EXCEPT:

Accepted Answer

A)  Collect the appropriate quantity of specimen. 
B)  Label the specimen accurately with the specific anatomic site and the patient information. 
C)  Select the correct anatomic site to collect the specimen. 
D)  It is acceptable to delay transport of the specimen to the laboratory if in transport media. 
A)  Collect the appropriate quantity of specimen. 
B)  Label the specimen accurately with the specific anatomic site and the patient information. 
C)  Select the correct anatomic site to collect the specimen. 
D)  It is acceptable to delay transport of the specimen to the laboratory if in transport media.

Question 11

The goal of the specimen collector when collecting specimens for culture should be to:

Accepted Answer

A)  Make sure the specimen gets to the lab. 
B)  Avoid hurting the patient when collecting the specimen. 
C)  Maintain the viability of the living organisms at the site with minimal contamination. 
D)  Get the specimen quickly to get the doctor off the collector's back. 
A)  Make sure the specimen gets to the lab. 
B)  Avoid hurting the patient when collecting the specimen. 
C)  Maintain the viability of the living organisms at the site with minimal contamination. 
D)  Get the specimen quickly to get the doctor off the collector's back.

Question 12

Routine primary plating media include all the following items EXCEPT:

Accepted Answer

A)  Special biochemical media 
B)  Nonselective agar plate 
C)  Selective and differential medium for enteric gram-negative bacilli for most routine bacterial cultures 
D)  Broth medium used as a supplement 
A)  Special biochemical media 
B)  Nonselective agar plate 
C)  Selective and differential medium for enteric gram-negative bacilli for most routine bacterial cultures 
D)  Broth medium used as a supplement

Question 13

Nonselective media supports:

Accepted Answer

A)  The grouping of microbes based on different characteristics demonstrated in the media 
B)  The growth of one type or group of microbes, but not another 
C)  The growth of most nonfastidious microbes 
D)  The growth of fastidious microbes through the addition of certain growth enhancers 
A)  The grouping of microbes based on different characteristics demonstrated in the media 
B)  The growth of one type or group of microbes, but not another 
C)  The growth of most nonfastidious microbes 
D)  The growth of fastidious microbes through the addition of certain growth enhancers

Question 14

The direct microscopic examination is a useful tool for all the following reasons EXCEPT:

Accepted Answer

A)  It can tell the physician what type of normal flora is present on the smear and if it is anaerobic or aerobic. 
B)  It can be used to determine the quality of the specimen. 
C)  It can give the microbiology technologist and the physician an indication of the infectious process involved. 
D)  It can dictate the need for nonroutine or additional testing. 
A)  It can tell the physician what type of normal flora is present on the smear and if it is anaerobic or aerobic. 
B)  It can be used to determine the quality of the specimen. 
C)  It can give the microbiology technologist and the physician an indication of the infectious process involved. 
D)  It can dictate the need for nonroutine or additional testing.

Question 15

All of the following are examples of suboptimal specimens that must be rejected EXCEPT:

Accepted Answer

A)  The information on the requisition does not match the information on the specimen label. 
B)  The specimen is for a Neisseria gonorrhea (G
C)  culture and submitted in a JEMBEC system. 
C)  The specimen container is leaking when received in the laboratory. 
D)  The specimen is received in a fixative solution such as formalin. 
A)  The information on the requisition does not match the information on the specimen label. 
B)  The specimen is for a Neisseria gonorrhea (G
C)  culture and submitted in a JEMBEC system. 
C)  The specimen container is leaking when received in the laboratory. 
D)  The specimen is received in a fixative solution such as formalin.

Question 16

The purpose of transport media is to:

Accepted Answer

A)  Make sure the microorganisms can multiply and live as though they were still in the host 
B)  Keep the swab moist so that the microorganisms do not dry out 
C)  Not promote the multiplication of microorganisms but ensure their preservation 
D)  Take the place of plating a routine culture with the specimen 
A)  Make sure the microorganisms can multiply and live as though they were still in the host 
B)  Keep the swab moist so that the microorganisms do not dry out 
C)  Not promote the multiplication of microorganisms but ensure their preservation 
D)  Take the place of plating a routine culture with the specimen

Question 17

Three types of specimens can be batch processed,including all the following EXCEPT:

Accepted Answer

A)  Specimens for Neisseria gonorrhea (G
C)  cultures 
B)  Specimens for acid-fast bacillus (AF
B)  cultures 
C)  Stool specimens for ova and parasite tests that are collected in preservatives 
D)  Specimens for viral culture collected in viral transport media 
A)  Specimens for Neisseria gonorrhea (G
C)  cultures 
B)  Specimens for acid-fast bacillus (AF
B)  cultures 
C)  Stool specimens for ova and parasite tests that are collected in preservatives 
D)  Specimens for viral culture collected in viral transport media

Exam 6: Specimen Collection and Processing

If a test is not considered appropriate for the specimen,the following should happen:

The specimen of choice for detecting gastrointestinal pathogens is:

All specimens must be transported:

Why is a clean-catch midstream urine used for a urine culture as opposed to a clean-catch urine?

To perform a quality laboratory analysis,the laboratory needs specific information regarding the patient and the specimen.What can be a critical weak link in the specimen management process?

Two types of specimens can use preservatives to maintain them until they can be delivered to the laboratory.They are:

What method is used to concentrate specimens to increase recovery of pathogens in the microbiology laboratory?

How is culture media selected for specimen plating?

Nonroutine specimens can include all the following specimen types EXCEPT:

All the following principles of specimen collection are fundamental to ensuring appropriate specimen management EXCEPT:

The goal of the specimen collector when collecting specimens for culture should be to:

Routine primary plating media include all the following items EXCEPT:

Nonselective media supports:

The direct microscopic examination is a useful tool for all the following reasons EXCEPT:

All of the following are examples of suboptimal specimens that must be rejected EXCEPT:

The purpose of transport media is to:

Three types of specimens can be batch processed,including all the following EXCEPT:

Bacterial Cell Structure, physiology, Metabolism, and Genetics

Host-Parasite Interaction

Laboratory Role in Infection Control

Control of Microorganisms

Performance Improvement in the Microbiology Laboratory

Microscopic Examination of Infected Materials

Use of Colonial Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

Applications of Molecular Diagnostics

Antibiotic Mechanisms of Action and Resistance

Antimicrobial Susceptibility Testing

Staphylococci

Streptococcus, enterococcus, and Other Catalase-Negative Gram-Positive Cocci

Aerobic Gram-Positive Bacilli

Neisseria Species and Moraxella Catarrhalis

Haemophilus and Other Fastidious Gram-Negative Bacilli

Enterobacteriaceae

Vibrio,aeromonas,plesiomonas and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

Anaerobes of Clinical Importance

Spirochetes

Chlamydia and Rickettsia

Mycoplasma and Ureaplasma

Mycobacterium Tuberculosis and Other Nontuberculosis Mycobacteria

Medically Significant Fungi

Diagnostic Parasitology

Clinical Virology

Agents of Bioterror

Biofilms: Architects of Disease

Upper and Lower Respiratory Tract Infections

Skin and Soft Tissue Infections

Gastrointestinal Infections and Food Poisoning

Infections of the Central Nervous System

Bacteremia and Sepsis

Urinary Tract Infections

Genital Infections and Sexually Transmitted Diseases

Infections in Special Populations

Zoonotic Diseases

Ocular Infections

Filters