Question 1

This microscope produces an image of a light cell against a dark background;internal structures are NOT visible.

Accepted Answer

A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope 
A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope

Question 2

An acidic dye

Accepted Answer

A) has a positively charged chromophore. 
B) has a negatively charged chromophore. 
C) is used to stain the background in a negative stain. 
D) is used to stain nuclei. 
E) has a negatively charged chromophore and is used to stain the background in a negative stain. 
A) has a positively charged chromophore. 
B) has a negatively charged chromophore. 
C) is used to stain the background in a negative stain. 
D) is used to stain nuclei. 
E) has a negatively charged chromophore and is used to stain the background in a negative stain.

Question 3

In using this microscope,the observer does NOT look directly at an image through a lens.

Accepted Answer

A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope 
A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope

Question 4

You find colorless areas in cells in a Gram-stained smear.What should you do next?

Accepted Answer

A) an acid-fast stain 
B) a flagella stain 
C) a capsule stain 
D) an endospore stain 
E) a simple stain 
A) an acid-fast stain 
B) a flagella stain 
C) a capsule stain 
D) an endospore stain 
E) a simple stain

Question 5

Scanned probe microscopy is used to examine fine detail of molecular complexes,such as blood clots,or molecules,such as DNA.

Accepted Answer

A) True 
 B)False

Question 6

In microscopy,the term resolution

Accepted Answer

A) refers to the ability to distinguish fine structure and detail in a specimen. 
B) is the same as the total magnification of a specimen. 
C) is improved when longer wavelengths of light are employed. 
D) is only observed in stained specimens. 
E) refers to magnification when using the electron microscope. 
A) refers to the ability to distinguish fine structure and detail in a specimen. 
B) is the same as the total magnification of a specimen. 
C) is improved when longer wavelengths of light are employed. 
D) is only observed in stained specimens. 
E) refers to magnification when using the electron microscope.

Question 7

A microorganism measures 5 μm in length.Its length in mm would be

Accepted Answer

A) 500 mm. 
B) 50 mm. 
C) 0.5 mm. 
D) 0.05 mm. 
E) 0.005 mm. 
A) 500 mm. 
B) 50 mm. 
C) 0.5 mm. 
D) 0.05 mm. 
E) 0.005 mm.

Question 8

Simple staining is often necessary to improve contrast in which microscope?

Accepted Answer

A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope 
A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope

Question 9

Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?

Accepted Answer

A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope 
A) compound light microscope 
B) phase-contrast microscope 
C) darkfield microscope 
D) fluorescence microscope 
E) electron microscope

Question 10

The purpose of the ocular lens is to

Accepted Answer

A) improve resolution. 
B) magnify the image from the objective lens. 
C) decrease the refractive index. 
D) increase the light. 
E) decrease the light. 
A) improve resolution. 
B) magnify the image from the objective lens. 
C) decrease the refractive index. 
D) increase the light. 
E) decrease the light.

Question 11

Which of the following places the steps in the correct sequence? 1-Staining
2-Making a smear
3-Fixing

Accepted Answer

A) 1-2-3 
B) 3-2-1 
C) 2-3-1 
D) 1-3-2 
E) The order is unimportant. 
A) 1-2-3 
B) 3-2-1 
C) 2-3-1 
D) 1-3-2 
E) The order is unimportant.

Question 12

Which of the following pairs is mismatched?

Accepted Answer

A) confocal microscope - produces a three-dimensional image 
B) darkfield microscope - uses visible light 
C) fluorescence microscope - uses a fluorescent light 
D) scanning electron microscope - produces a three-dimensional image 
E) scanning tunneling microscope - allows visualization of atoms 
A) confocal microscope - produces a three-dimensional image 
B) darkfield microscope - uses visible light 
C) fluorescence microscope - uses a fluorescent light 
D) scanning electron microscope - produces a three-dimensional image 
E) scanning tunneling microscope - allows visualization of atoms

Question 13

Immersion oil is necessary when using the 100x objective on a compound light microscope

Accepted Answer

A) to lubricate the objective. 
B) to prevent the slide from scratching the lens. 
C) otherwise the image will be fuzzy. 
D) otherwise too much light is lost to refraction. 
E) otherwise too much light is lost to refraction and the image will be fuzzy. 
A) to lubricate the objective. 
B) to prevent the slide from scratching the lens. 
C) otherwise the image will be fuzzy. 
D) otherwise too much light is lost to refraction. 
E) otherwise too much light is lost to refraction and the image will be fuzzy.

Question 14

Which microscope uses visible light?

Accepted Answer

A) confocal microscope 
B) differential interference contrast microscope 
C) fluorescence microscope 
D) scanning acoustic microscope 
E) scanning electron microscope 
A) confocal microscope 
B) differential interference contrast microscope 
C) fluorescence microscope 
D) scanning acoustic microscope 
E) scanning electron microscope

Question 15

What structure does light pass through after leaving the condenser in a compound light microscope?

Accepted Answer

A) ocular lens 
B) objective lens 
C) specimen 
D) illuminator 
A) ocular lens 
B) objective lens 
C) specimen 
D) illuminator

Question 16

What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?

Accepted Answer

A) 4.5x 
B) 10x 
C) 45x 
D) 100x 
E) 450x 
A) 4.5x 
B) 10x 
C) 45x 
D) 100x 
E) 450x

Question 17

The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

Accepted Answer

A) True 
 B)False

Question 18

Figure 3.1  
In Figure 3.1,line "c" points to the microscope's

Accepted Answer

A) illuminator. 
B) condenser. 
C) ocular lens. 
D) objective lens. 
A) illuminator. 
B) condenser. 
C) ocular lens. 
D) objective lens.

Question 19

Which of the following pairs is mismatched?

Accepted Answer

A) alcohol-acetone - decolorizer 
B) crystal violet - basic dye 
C) safranin - acid dye 
D) iodine - mordant 
E) carbolfuchsin - basic dye 
A) alcohol-acetone - decolorizer 
B) crystal violet - basic dye 
C) safranin - acid dye 
D) iodine - mordant 
E) carbolfuchsin - basic dye

Question 20

In a completed Gram stain,gram-negative bacteria are colorless.

Accepted Answer

A) True 
 B)False

Exam 3: Observing Microorganisms Through a Microscope

This microscope produces an image of a light cell against a dark background;internal structures are NOT visible.

An acidic dye

In using this microscope,the observer does NOT look directly at an image through a lens.

You find colorless areas in cells in a Gram-stained smear.What should you do next?

Scanned probe microscopy is used to examine fine detail of molecular complexes,such as blood clots,or molecules,such as DNA.

In microscopy,the term resolution

A microorganism measures 5 μm in length.Its length in mm would be

Simple staining is often necessary to improve contrast in which microscope?

Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?

The purpose of the ocular lens is to

Which of the following places the steps in the correct sequence? 1-Staining 2-Making a smear 3-Fixing

Which of the following pairs is mismatched?

Immersion oil is necessary when using the 100x objective on a compound light microscope

Which microscope uses visible light?

What structure does light pass through after leaving the condenser in a compound light microscope?

What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?

The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

Figure 3.1 In Figure 3.1,line "c" points to the microscope's

Which of the following pairs is mismatched?

In a completed Gram stain,gram-negative bacteria are colorless.

The Microbial World and You

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Functional Anatomy of Prokaryotic and Eukaryotic

Microbial Metabolism

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Classification of Microorganisms

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Principles of Disease and Epidemiology

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Innate Immunity: Nonspecific Defenses of the Host

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Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

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