Exam 1: Introduction to Virology

If the genome of a positive-strand RNA virus, which has been purified away from all of the virion proteins, was injected into the cytoplasm of an appropriate host cell, what would happen first?

(Multiple Choice)

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Question 21

What does the word "virus" mean in Latin?

(Multiple Choice)

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Question 22

To start an experiment, you do an infection by putting 0.5 ml of a virus stock that has a titer of 6 x 10⁷ pfu/ml onto a plate of animal cells that contains 5 x 10⁶ cells.What is the multiplicity of infection for this experiment?

(Multiple Choice)

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Question 23

What is another term for an RNA-dependent-DNA-polymerase?

(Multiple Choice)

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Question 24

When doing a plaque assay with human or animal cells, a layer of nutrient media mixed with agar is put on top of the cells after they have been infected with the diluted virus.What is the major purpose of this agar layer?

(Multiple Choice)

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Question 25

You are given a solution of virus and asked to determine the titer.You carry out a plaque assay with 0.5mls of a 10⁵ dilution of the virus solution and obtain an average of 150 plaques.What is the titer of the solution?

(Multiple Choice)

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Question 26

Most DNA viruses that replicate in the nucleus of the host cell use which of the following to transcribe their genes into mRNA?

(Multiple Choice)

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Question 27

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Exam 1: Introduction to Virology

If the genome of a positive-strand RNA virus, which has been purified away from all of the virion proteins, was injected into the cytoplasm of an appropriate host cell, what would happen first?

What does the word "virus" mean in Latin?

To start an experiment, you do an infection by putting 0.5 ml of a virus stock that has a titer of 6 x 10⁷ pfu/ml onto a plate of animal cells that contains 5 x 10⁶ cells.What is the multiplicity of infection for this experiment?

What is another term for an RNA-dependent-DNA-polymerase?

When doing a plaque assay with human or animal cells, a layer of nutrient media mixed with agar is put on top of the cells after they have been infected with the diluted virus.What is the major purpose of this agar layer?

You are given a solution of virus and asked to determine the titer.You carry out a plaque assay with 0.5mls of a 10⁵ dilution of the virus solution and obtain an average of 150 plaques.What is the titer of the solution?

Most DNA viruses that replicate in the nucleus of the host cell use which of the following to transcribe their genes into mRNA?

Virus Structure and Assembly

Virus Classification: The World of Viruses

Virus Entry

Single-Stranded RNA Bacteriophages

Microviruses

Bacteriophage

Bacteriophage Lambda

Viruses of Archaea

Cucumber Mosaic Virus

Picornaviruses

Flaviviruses

Togaviruses

Coronaviruses

Paramyxo-Viruses and Rhabdoviruses

Filovirouses

Bunyaviruses

Influenza Viruses

Reoviruses

Parvoviruses

Polyomaviruses

Papillomaviruses

Adenoviruses

Herpesviruses

Baculoviruses

Poxviruses

Viruses of Algae and Mimivirus

Retroviruses

Human Immunodeficiency

Hepadnaviruses

Viroids and Hepatitis Delta Virus

Prions

Intrinsic Cellular Defenses Against Virus Infection

Innate and Adaptive Immune Responses to Virus Infection

Antiviral Vaccines

Antiviral Chemotherapy

Eukaryotic Virus Vectors

Filters