Question 1

Bacterial growth in colonies on agar plates _____.

Accepted Answer

A) have all phases of growth occurring simultaneously somewhere in the colony 
B) most growth occurs at the center of the colony 
C) involves budding of small new colonies from the surface of an existing colony 
D) have lag phase cells in the center of the colony,surrounded by a ring of cells in exponential phase 
A) have all phases of growth occurring simultaneously somewhere in the colony 
B) most growth occurs at the center of the colony 
C) involves budding of small new colonies from the surface of an existing colony 
D) have lag phase cells in the center of the colony,surrounded by a ring of cells in exponential phase A

Question 2

Organisms with special nutritional needs are said to be _____.

Accepted Answer

A) barophiles 
B) fastidious 
C) aerobes 
D) none of these 
A) barophiles 
B) fastidious 
C) aerobes 
D) none of these B

Question 3

Which of the following does not contribute to endospore resistance to unfavorable conditions

Accepted Answer

A) Dipicolinic acid and calcium ions in the core 
B) Cortex 
C) Spore coat 
D) Germination proper 
A) Dipicolinic acid and calcium ions in the core 
B) Cortex 
C) Spore coat 
D) Germination proper D

Question 4

All of the following are ways microorganisms adapt to limited nutrients except:

Accepted Answer

A) synthesize increased amount of enzymes for uptake and metabolism of limited nutrients 
B) form metabolically active highly resistant endospores 
C) synthesize enzymes needed to use a different nutrient source 
D) adjust the rate at which they metabolize nutrients 
A) synthesize increased amount of enzymes for uptake and metabolism of limited nutrients 
B) form metabolically active highly resistant endospores 
C) synthesize enzymes needed to use a different nutrient source 
D) adjust the rate at which they metabolize nutrients

Question 5

Where would you expect to find a barophile

Accepted Answer

A) At the bottom of the ocean 
B) In unpasteurized milk 
C) In the large intestine 
D) In sewage 
A) At the bottom of the ocean 
B) In unpasteurized milk 
C) In the large intestine 
D) In sewage

Question 6

Most human pathogens are _____.

Accepted Answer

A) psychrophiles 
B) mesophiles 
C) thermophiles 
D) acidophiles 
A) psychrophiles 
B) mesophiles 
C) thermophiles 
D) acidophiles

Question 7

A decrease in the amount of nitrogen to a culture of Clostridium botulinum would induce _____.

Accepted Answer

A) activation 
B) germination 
C) sporulation 
D) outgrowth 
A) activation 
B) germination 
C) sporulation 
D) outgrowth

Question 8

A growth medium consisting of only known amounts of water,magnesium,calcium chloride,potassium and glucose would be considered:

Accepted Answer

A) a defined synthetic medium 
B) a complex medium 
C) a chemically nondefined medium 
D) two of these 
A) a defined synthetic medium 
B) a complex medium 
C) a chemically nondefined medium 
D) two of these

Question 9

Microbial growth:

Accepted Answer

A) refers to the increase in the size of a microbial cell. 
B) refers to the increase in the frequency of cell division. 
C) in a single generation time leads to double the cell size. 
D) in a single generation time leads to double the number of microbes. 
A) refers to the increase in the size of a microbial cell. 
B) refers to the increase in the frequency of cell division. 
C) in a single generation time leads to double the cell size. 
D) in a single generation time leads to double the number of microbes.

Question 10

The type of cell reproduction in which a small,new cell develops from the surface of an existing cell and then separates from the parent cell is known as ________ and is the normal mode of replication in _____ .

Accepted Answer

A) binary fission,bacteria 
B) binary fission,yeast 
C) budding,bacteria 
D) budding,yeast 
A) binary fission,bacteria 
B) binary fission,yeast 
C) budding,bacteria 
D) budding,yeast

Question 11

During this phase,organisms produce large amounts of ATP.

Accepted Answer

A) A 
B) B 
C) C 
D) D 
A) A 
B) B 
C) C 
D) D

Question 12

A culture that contains only a single species of organism is known as a _____.

Accepted Answer

A) pure culture 
B) Koch culture 
C) mixed culture 
D) contaminated culture 
A) pure culture 
B) Koch culture 
C) mixed culture 
D) contaminated culture

Question 13

Microaerophiles:

Accepted Answer

A) grow best when the environment has a small amount of carbon dioxide. 
B) grow best when the environment has a small amount of free oxygen. 
C) need a high concentration of carbon dioxide. 
D) need a high concentration of oxygen. 
A) grow best when the environment has a small amount of carbon dioxide. 
B) grow best when the environment has a small amount of free oxygen. 
C) need a high concentration of carbon dioxide. 
D) need a high concentration of oxygen.

Question 14

Serial dilutions are used to:

Accepted Answer

A) slow down the growth of microbes 
B) speed up the growth of microbes 
C) obtain bacterial cultures at several different concentrations 
D) form bacterial colonies 
A) slow down the growth of microbes 
B) speed up the growth of microbes 
C) obtain bacterial cultures at several different concentrations 
D) form bacterial colonies

Question 15

A growth medium consisting of only water,glucose and beef extract would be considered _____.

Accepted Answer

A) a defined synthetic medium 
B) a complex medium 
C) a chemically defined medium 
D) two of these 
A) a defined synthetic medium 
B) a complex medium 
C) a chemically defined medium 
D) two of these

Question 16

Turbidity in a bacterial culture can be measured using a/an:

Accepted Answer

A) incubator 
B) colony county 
C) spectrophotometer 
D) chemostat 
A) incubator 
B) colony county 
C) spectrophotometer 
D) chemostat

Question 17

Microbes that live in this environment actively transport _____ out of their cells.

Accepted Answer

A) potassium 
B) oxygen 
C) sodium 
D) nitrogen 
A) potassium 
B) oxygen 
C) sodium 
D) nitrogen

Question 18

The pour plate method of isolating pure cultures _____.

Accepted Answer

A) relies on the fact that liquid agar will solidify as it cools 
B) is useful for growing microaerophiles 
C) results in some organisms growing embedded in the agar 
D) all of these 
A) relies on the fact that liquid agar will solidify as it cools 
B) is useful for growing microaerophiles 
C) results in some organisms growing embedded in the agar 
D) all of these

Question 19

Capnophiles grow best under conditions of _____.

Accepted Answer

A) high carbon dioxide 
B) low carbon dioxide 
C) high osmotic pressure 
D) low osmotic pressure 
A) high carbon dioxide 
B) low carbon dioxide 
C) high osmotic pressure 
D) low osmotic pressure

Question 20

An obligate psychrophile would produce a turbid culture at ________ degrees Celsius.

Accepted Answer

A) 15 
B) 30 
C) 45 
D) 60 
A) 15 
B) 30 
C) 45 
D) 60

Exam 7: Growth and Culturing of Bacteria

Bacterial growth in colonies on agar plates _____.

Organisms with special nutritional needs are said to be _____.

Which of the following does not contribute to endospore resistance to unfavorable conditions

All of the following are ways microorganisms adapt to limited nutrients except:

Where would you expect to find a barophile

Most human pathogens are _____.

A decrease in the amount of nitrogen to a culture of Clostridium botulinum would induce _____.

A growth medium consisting of only known amounts of water,magnesium,calcium chloride,potassium and glucose would be considered:

Microbial growth:

The type of cell reproduction in which a small,new cell develops from the surface of an existing cell and then separates from the parent cell is known as ________ and is the normal mode of replication in _____ .

During this phase,organisms produce large amounts of ATP.

A culture that contains only a single species of organism is known as a _____.

Microaerophiles:

Serial dilutions are used to:

A growth medium consisting of only water,glucose and beef extract would be considered _____.

Turbidity in a bacterial culture can be measured using a/an:

Microbes that live in this environment actively transport _____ out of their cells.

The pour plate method of isolating pure cultures _____.

Capnophiles grow best under conditions of _____.

An obligate psychrophile would produce a turbid culture at ________ degrees Celsius.

Scope and History of Microbiology

The Microbiome

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: The Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Basic Principles of Adaptive Immunity and Immunization

Immune Disorders

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, Lymphatic, and Systemic Diseases

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

The type of cell reproduction in which a small,new cell develops from the surface of an existing cell and then separates from the parent cell is known as ____ and is the normal mode of replication in _ .