Question 1

Starch would be broken down to maltose by which of the following exoenzymes

Accepted Answer

A) gelatinase 
B) amylase 
C) caseinase 
D) lipase 
A) gelatinase 
B) amylase 
C) caseinase 
D) lipase

Question 2

Which of the following is not a stage in endospores returning to the vegetative state

Accepted Answer

A) Traumatic agent such as low pH or heat damages the coat. 
B) Outgrowth occurs in a medium with adequate nutrients. 
C) Divisions of the endospores early in their development. 
D) Water and nutrients penetrates damaged coat. 
A) Traumatic agent such as low pH or heat damages the coat. 
B) Outgrowth occurs in a medium with adequate nutrients. 
C) Divisions of the endospores early in their development. 
D) Water and nutrients penetrates damaged coat.

Question 3

A bacterial culture can be kept in the log phase of growth indefinitely with the help of a/an:

Accepted Answer

A) incubator 
B) chemostat 
C) spectrophotometer 
D) colony counter 
A) incubator 
B) chemostat 
C) spectrophotometer 
D) colony counter

Question 4

The streak plate method produces pure cultures by separating individual bacterial cells from one another by _____.

Accepted Answer

A) dragging them across a solid surface 
B) dispersing them in liquid media 
C) killing off those cells that are not able to survive in hot agar 
D) embedding some cells in the agar so that they are exposed to lower concentrations of oxygen 
A) dragging them across a solid surface 
B) dispersing them in liquid media 
C) killing off those cells that are not able to survive in hot agar 
D) embedding some cells in the agar so that they are exposed to lower concentrations of oxygen

Question 5

When cells are placed in a hypertonic environment they will undergo ________ .

Accepted Answer

A) lysis 
B) no change in size 
C) swelling of the cell which is contained by the cell wall 
D) plasmolysis 
A) lysis 
B) no change in size 
C) swelling of the cell which is contained by the cell wall 
D) plasmolysis

Question 6

Acidophiles would be expected to grow best at a pH of _____.

Accepted Answer

A) 3 
B) 6 
C) 9 
D) 12 
A) 3 
B) 6 
C) 9 
D) 12

Question 7

Endospores are:

Accepted Answer

A) metabolically active 
B) reproductive structures 
C) found mostly in Gram negative organisms 
D) protective structures 
A) metabolically active 
B) reproductive structures 
C) found mostly in Gram negative organisms 
D) protective structures

Question 8

Nonculturable organisms:

Accepted Answer

A) are rare. 
B) can be identified based on their DNA. 
C) can only be seen on selective agar plates. 
D) have defined generation times. 
A) are rare. 
B) can be identified based on their DNA. 
C) can only be seen on selective agar plates. 
D) have defined generation times.

Question 9

A media on which Gram positive organism turn green and Gram negative organisms turn blue would be _____.

Accepted Answer

A) selective 
B) differential 
C) complex 
D) enriched 
A) selective 
B) differential 
C) complex 
D) enriched

Question 10

Which of the following bacterial counting techniques relies on a statistical estimate to determine the of bacteria in a culture

Accepted Answer

A) Serial dilution 
B) Standard plate count 
C) Spread plate 
D) Most probable number 
A) Serial dilution 
B) Standard plate count 
C) Spread plate 
D) Most probable number

Question 11

Which of the following statements about bacterial growth is false

Accepted Answer

A) Agar is used as a solidifying agent in some types of media. 
B) Bacteria growing in a liquid culture will generate colonies. 
C) A turbid culture is indicative of bacterial growth. 
D) Each bacterium plated will represent a colony-forming unit. 
A) Agar is used as a solidifying agent in some types of media. 
B) Bacteria growing in a liquid culture will generate colonies. 
C) A turbid culture is indicative of bacterial growth. 
D) Each bacterium plated will represent a colony-forming unit.

Question 12

Sporulation occurs in:

Accepted Answer

A) Clostridium 
B) Staphylocoocus 
C) Klebsiella 
D) Citrobacter 
A) Clostridium 
B) Staphylocoocus 
C) Klebsiella 
D) Citrobacter

Question 13

Exoenzymes:

Accepted Answer

A) produced by gram positive rods act in the medium around the organism 
B) are released by the golgi apparatus into the cytoplasm where they act 
C) produced by gram negative rods act in the periplasmic space 
D) two of these 
A) produced by gram positive rods act in the medium around the organism 
B) are released by the golgi apparatus into the cytoplasm where they act 
C) produced by gram negative rods act in the periplasmic space 
D) two of these

Question 14

Chocolate agar:

Accepted Answer

A) contains blood 
B) is used exclusively for stock cultures 
C) is nutrient poor 
D) is a selective agar plate 
A) contains blood 
B) is used exclusively for stock cultures 
C) is nutrient poor 
D) is a selective agar plate

Question 15

Halophiles require an environment with a high concentration of ________ for optimal growth.

Accepted Answer

A) sugar 
B) alcohol 
C) salt 
D) phosphorous 
A) sugar 
B) alcohol 
C) salt 
D) phosphorous

Exam 7: Growth and Culturing of Bacteria

Starch would be broken down to maltose by which of the following exoenzymes

Which of the following is not a stage in endospores returning to the vegetative state

A bacterial culture can be kept in the log phase of growth indefinitely with the help of a/an:

The streak plate method produces pure cultures by separating individual bacterial cells from one another by _____.

When cells are placed in a hypertonic environment they will undergo ________ .

Acidophiles would be expected to grow best at a pH of _____.

Endospores are:

Nonculturable organisms:

A media on which Gram positive organism turn green and Gram negative organisms turn blue would be _____.

Which of the following bacterial counting techniques relies on a statistical estimate to determine the of bacteria in a culture

Which of the following statements about bacterial growth is false

Sporulation occurs in:

Exoenzymes:

Chocolate agar:

Halophiles require an environment with a high concentration of ________ for optimal growth.

Scope and History of Microbiology

The Microbiome

Fundamentals of Chemistry

Microscopy and Staining

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: The Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Basic Principles of Adaptive Immunity and Immunization

Immune Disorders

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, Lymphatic, and Systemic Diseases

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters