Question 1

Differential media only allows certain bacteria to grow.

Accepted Answer

A) True 
 B)False

Question 2

The lag phase of the bacterial growth curve is marked by

Accepted Answer

A) a decrease in cell mass. 
B) dormant,metabolically inactive cells. 
C) metabolically active cells. 
D) vigorously dividing cells. 
A) a decrease in cell mass. 
B) dormant,metabolically inactive cells. 
C) metabolically active cells. 
D) vigorously dividing cells.

Question 3

A medium that inhibits the growth of organisms other than the one being sought is termed a(n)

Accepted Answer

A) synthetic medium. 
B) specific culture medium. 
C) selective medium. 
D) enrichment medium. 
A) synthetic medium. 
B) specific culture medium. 
C) selective medium. 
D) enrichment medium.

Question 4

Products that limit pH changes are often incorporated into media and are referred to as

Accepted Answer

A) enzymes. 
B) bases. 
C) buffers. 
D) acids. 
A) enzymes. 
B) bases. 
C) buffers. 
D) acids.

Question 5

MacConkey agar is

Accepted Answer

A) a selective agar. 
B) a differential agar. 
C) a selective and differential agar. 
D) used to distinguish between bacteria by the type of hemolysis observed. 
A) a selective agar. 
B) a differential agar. 
C) a selective and differential agar. 
D) used to distinguish between bacteria by the type of hemolysis observed.

Question 6

It is assumed that every colony observed on a streak plate arose from a single bacterium.

Accepted Answer

A) True 
 B)False

Question 7

Bacteria on fish caught in the Arctic Ocean would

Accepted Answer

A) be psychrophiles. 
B) be mesophiles. 
C) continue to grow while the fish is in the refrigerator. 
D) not grow very well in the refrigerator. 
E) be psychrophiles AND continue to grow while the fish is in the refrigerator. 
A) be psychrophiles. 
B) be mesophiles. 
C) continue to grow while the fish is in the refrigerator. 
D) not grow very well in the refrigerator. 
E) be psychrophiles AND continue to grow while the fish is in the refrigerator.

Question 8

Shake tubes are used to determine the

Accepted Answer

A) pH requirements for bacterial growth. 
B) temperature requirements for bacterial growth. 
C) oxygen requirements for bacterial growth. 
D) salt requirements for bacterial growth. 
A) pH requirements for bacterial growth. 
B) temperature requirements for bacterial growth. 
C) oxygen requirements for bacterial growth. 
D) salt requirements for bacterial growth.

Question 9

You are a microbiologist working for a pharmaceutical company and discover a new secreted metabolite that can serve as a medication.Your company asks you to oversee the production of the metabolite.Which of the following is something that is NOT important to consider if you need to grow 5,000 liter cultures of bacteria for the purpose of harvesting the metabolite they secrete?

Accepted Answer

A) The death rate of the bacteria after stationary phase is complete. 
B) The ideal rate of input of new nutrients into the culture to maintain the cells in log phase. 
C) The ideal rate of pulling off some of the culture in order to maintain the cells in log phase. 
D) The best way to mix the large vat in order to keep it homogenous (constant throughout)in terms of nutrients,temperature,and oxygen levels. 
E) The best way to keep the pH of the entire mixture at the ideal level to promote log phase growth. 
A) The death rate of the bacteria after stationary phase is complete. 
B) The ideal rate of input of new nutrients into the culture to maintain the cells in log phase. 
C) The ideal rate of pulling off some of the culture in order to maintain the cells in log phase. 
D) The best way to mix the large vat in order to keep it homogenous (constant throughout)in terms of nutrients,temperature,and oxygen levels. 
E) The best way to keep the pH of the entire mixture at the ideal level to promote log phase growth.

Question 10

Trace elements

Accepted Answer

A) include zinc,copper,and manganese. 
B) are required in large amounts. 
C) may be needed for enzyme function. 
D) are involved in maintaining pH in the cell. 
E) include zinc,copper,and manganese AND may be needed for enzyme function. 
A) include zinc,copper,and manganese. 
B) are required in large amounts. 
C) may be needed for enzyme function. 
D) are involved in maintaining pH in the cell. 
E) include zinc,copper,and manganese AND may be needed for enzyme function.

Question 11

Agar replaced gelatin as the gelling (solidifying)agent for media because

Accepted Answer

A) agar is much cheaper. 
B) much fewer bacteria can break down agar than gelatin. 
C) agar is solid at body temperature. 
D) gelatin became unavailable during World War II. 
E) much fewer bacteria can break down agar than gelatin AND agar is solid at body temperature. 
A) agar is much cheaper. 
B) much fewer bacteria can break down agar than gelatin. 
C) agar is solid at body temperature. 
D) gelatin became unavailable during World War II. 
E) much fewer bacteria can break down agar than gelatin AND agar is solid at body temperature.

Question 12

Secondary metabolites may be antibiotics.

Accepted Answer

A) True 
 B)False

Question 13

Prokaryotes are the only organisms able to use atmospheric nitrogen as a nitrogen source.

Accepted Answer

A) True 
 B)False

Question 14

All the bacterial cells that result from the replication of a single original bacterial organism are said to be a

Accepted Answer

A) population. 
B) pure culture. 
C) lag culture. 
D) mutant culture. 
A) population. 
B) pure culture. 
C) lag culture. 
D) mutant culture.

Question 15

The simplest technique for isolating bacteria in growth media is referred to as the

Accepted Answer

A) pour-plate method. 
B) streak-plate method. 
C) serial dilution method. 
D) MPN method. 
A) pour-plate method. 
B) streak-plate method. 
C) serial dilution method. 
D) MPN method.

Question 16

The scientist that contributed most to the development of pure culture techniques was

Accepted Answer

A) Alexander Fleming. 
B) Louis Pasteur. 
C) Edward Jenner. 
D) Robert Koch. 
A) Alexander Fleming. 
B) Louis Pasteur. 
C) Edward Jenner. 
D) Robert Koch.

Question 17

Freezing is an effective means of destroying bacteria.

Accepted Answer

A) True 
 B)False

Question 18

Medically important bacteria are often

Accepted Answer

A) grown on agar containing blood. 
B) grown at 37$\circ$C. 
C) grown on agar containing chocolate. 
D) grown at a pH of 5. 
E) grown on agar containing blood AND grown at 37$\circ$C. 
A) grown on agar containing blood. 
B) grown at 37$\circ$C. 
C) grown on agar containing chocolate. 
D) grown at a pH of 5. 
E) grown on agar containing blood AND grown at 37$\circ$C.

Question 19

Candle jars are usually used to

Accepted Answer

A) store candles. 
B) provide an atmosphere with CO2. 
C) stimulate the growth of obligate anaerobes. 
D) prevent the growth of obligate aerobes. 
E) provide an atmosphere with CO2 AND stimulate the growth of obligate anaerobes. 
A) store candles. 
B) provide an atmosphere with CO2. 
C) stimulate the growth of obligate anaerobes. 
D) prevent the growth of obligate aerobes. 
E) provide an atmosphere with CO2 AND stimulate the growth of obligate anaerobes.

Question 20

The solidifying agent used most successfully in bacterial nutrient media is

Accepted Answer

A) gelatin. 
B) peptone. 
C) agar. 
D) starch. 
A) gelatin. 
B) peptone. 
C) agar. 
D) starch.

Exam 4: Dynamics of Prokaryotic Growth

Differential media only allows certain bacteria to grow.

The lag phase of the bacterial growth curve is marked by

A medium that inhibits the growth of organisms other than the one being sought is termed a(n)

Products that limit pH changes are often incorporated into media and are referred to as

MacConkey agar is

It is assumed that every colony observed on a streak plate arose from a single bacterium.

Bacteria on fish caught in the Arctic Ocean would

Shake tubes are used to determine the

Trace elements

Agar replaced gelatin as the gelling (solidifying)agent for media because

Secondary metabolites may be antibiotics.

Prokaryotes are the only organisms able to use atmospheric nitrogen as a nitrogen source.

All the bacterial cells that result from the replication of a single original bacterial organism are said to be a

The simplest technique for isolating bacteria in growth media is referred to as the

The scientist that contributed most to the development of pure culture techniques was

Freezing is an effective means of destroying bacteria.

Medically important bacteria are often

Candle jars are usually used to

The solidifying agent used most successfully in bacterial nutrient media is

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From DNA to Protein

Bacterial Genetics

Biotechnology and Recombinant DNA

Identification and Classification of Prokaryotic Organisms

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids, and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Tract Infections

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