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The DNA Strand 3'0-GACTATTCCGACCC-5' Is Being Sequenced Using the Dideoxy

Question 33

Multiple Choice

The DNA strand 3'0-GACTATTCCGACCC-5' is being sequenced using the dideoxy method.One reaction mixture contains all four deoxynucleoside triphosphates (dATP, dTTP, dCTP, dGTP) and a small amount (10%) of dideoxythymidine (ddTTP) .After synthesis is completed, what sized bands will appear in the gel? (Assume that the primer is present but not counted in the size of any of the bands.)


A) 2-bp, 5-bp, and 11-bp bands
B) 2-bp, 11-bp, 12-bp, 13-bp, and 14-bp bands
C) 4-bp band only
D) 4-bp, 6-bp, and 7-bp bands
E) 1-bp, 2-bp, 3-bp, and 4-bp bands

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