You Are Attempting to Determine Whether a Plant-Derived Processed Food

Essay

You are attempting to determine whether a plant-derived processed food sample (for example, a corn chip)contains material from a transgenic organism.First, you crush the sample and attempt to extract DNA from it.Next, you perform PCR using two different sets of primers.One primer set will amplify a DNA sequence present in all plants.The second primer set will amplify a DNA sequence only found in transgenic plants.
a.Why must you use both sets of primers for this experiment?
b.You obtain the results shown in the panel below.What conclusions can you draw from these reaction results? Does this test sample contain genetically modified components? Why or why not? [Lanes 1-6 are the same as listed above.]