You Are Trying to Determine Whether a Certain RFLP Marker

Essay

You are trying to determine whether a certain RFLP marker is linked to a specific disease gene in dogs.A cross between a diseased male and a healthy female produces nine offspring.You isolate genomic DNA from parents and offspring, digest with EcoRI, radiolabel a portion of RFLP marker to use as a probe, and perform a Southern analysis.The pedigree and autoradiogram results are shown in the following figure.Assume that the disease is an autosomal dominant. a.Explain the relationship between the crossing results (i.e., diseased versus healthy)and the RFLP patterns.
b.How would you explain the results seen for the second male puppy?