Question 1

A tick is usually measured in terms of

Accepted Answer

A)  meters (m). 
B)  millimeters (mm). 
C)  micrometers (µm). 
D)  nanometers (nm). 
E)  yards. 
A)  meters (m). 
B)  millimeters (mm). 
C)  micrometers (µm). 
D)  nanometers (nm). 
E)  yards.

Question 2

All of the following are common to both the Gram stain and the acid-fast stain EXCEPT

Accepted Answer

A)  primary stain. 
B)  counterstain. 
C)  a decolorizing agent. 
D)  a mordant. 
E)  a decolorizing agent and a counterstain. 
A)  primary stain. 
B)  counterstain. 
C)  a decolorizing agent. 
D)  a mordant. 
E)  a decolorizing agent and a counterstain.

Question 3

In a compound microscope, the lens that directs light through the specimen is the __________ lens.

Accepted Answer

The answer of In a compound microscope, the lens that...

Question 4

The most appropriate unit of measurement for intact archaea is the

Accepted Answer

A)  meter (m). 
B)  millimeter (mm). 
C)  micrometer (µm). 
D)  nanometer (nm). 
E)  centimeter (cm). 
A)  meter (m). 
B)  millimeter (mm). 
C)  micrometer (µm). 
D)  nanometer (nm). 
E)  centimeter (cm).

Question 5

Which of the following is NOT associated with an electron microscope?

Accepted Answer

A)  an electron beam 
B)  magnetic fields 
C)  a prism 
D)  a fluorescent screen 
E)  a vacuum 
A)  an electron beam 
B)  magnetic fields 
C)  a prism 
D)  a fluorescent screen 
E)  a vacuum

Question 6

In Gram staining, ethanol-acetone is used as a

Accepted Answer

A)  decolorizing agent. 
B)  counterstain. 
C)  mordant. 
D)  drying agent. 
E)  primary stain. 
A)  decolorizing agent. 
B)  counterstain. 
C)  mordant. 
D)  drying agent. 
E)  primary stain.

Question 7

Compare and contrast the three domains identified by Woese: Eukarya, Bacteria, and Archaea.

Accepted Answer

With respect to cell type, organisms in

Question 8

If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result?

Accepted Answer

A)  All cells would be purple. 
B)  Gram-positive cells would be purple, and Gram-negative cells would be colorless. 
C)  All cells would be pink. 
D)  Gram-positive cells would be pink, and Gram-negative cells would be purple. 
E)  Gram-positive cells would be colorless, and Gram-negative cells would be pink. 
A)  All cells would be purple. 
B)  Gram-positive cells would be purple, and Gram-negative cells would be colorless. 
C)  All cells would be pink. 
D)  Gram-positive cells would be pink, and Gram-negative cells would be purple. 
E)  Gram-positive cells would be colorless, and Gram-negative cells would be pink.

Question 9

Which of the following phenomena produces magnification?

Accepted Answer

A)  the wavelength of a radiation source 
B)  the refraction of radiation as it passes through a lens 
C)  the thickness of a microscopic specimen 
D)  the numerical aperture of a lens 
E)  the length of an objective lens 
A)  the wavelength of a radiation source 
B)  the refraction of radiation as it passes through a lens 
C)  the thickness of a microscopic specimen 
D)  the numerical aperture of a lens 
E)  the length of an objective lens

Question 10

The dimensions of a virus are usually described in

Accepted Answer

A)  meters (m). 
B)  millimeters (mm). 
C)  micrometers (µm). 
D)  nanometers (nm). 
E)  centimeters. 
A)  meters (m). 
B)  millimeters (mm). 
C)  micrometers (µm). 
D)  nanometers (nm). 
E)  centimeters.

Question 11

A patient suffering from tuberculosis could be diagnosed by use of the __________ stain.

Accepted Answer

A)  Gram 
B)  endospore 
C)  acid-fast 
D)  flagellar 
E)  capsule 
A)  Gram 
B)  endospore 
C)  acid-fast 
D)  flagellar 
E)  capsule

Question 12

Which of the following is NOT a characteristic of a genus name?

Accepted Answer

A)  it is usually an adjective. 
B)  it is written before the specific epithet. 
C)  it is always capitalized. 
D)  it is either underlined or in italics. 
E)  it is one of two names used to identify an organism. 
A)  it is usually an adjective. 
B)  it is written before the specific epithet. 
C)  it is always capitalized. 
D)  it is either underlined or in italics. 
E)  it is one of two names used to identify an organism.

Question 13

Why does immersion oil improve resolution?

Accepted Answer

A)  It allows light to travel at a uniform speed on its way to the lens. 
B)  It decreases the working distance. 
C)  It increases the numerical aperture. 
D)  It increases numerical aperture and maintains a uniform light speed. 
E)  It increases the angle of refraction of the light. 
A)  It allows light to travel at a uniform speed on its way to the lens. 
B)  It decreases the working distance. 
C)  It increases the numerical aperture. 
D)  It increases numerical aperture and maintains a uniform light speed. 
E)  It increases the angle of refraction of the light.

Question 14

Carbolfuchsin is the __________ in the acid-fast stain.

Accepted Answer

A)  primary stain 
B)  mordant 
C)  decolorizer 
D)  counterstain 
E)  fixing reagent 
A)  primary stain 
B)  mordant 
C)  decolorizer 
D)  counterstain 
E)  fixing reagent

Question 15

A structure that appears in a transmission electron micrograph but is not actually present in the specimen is known as a(n)

Accepted Answer

A)  antigen. 
B)  biofilm. 
C)  artifact. 
D)  refraction. 
E)  mordant. 
A)  antigen. 
B)  biofilm. 
C)  artifact. 
D)  refraction. 
E)  mordant.

Question 16

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Accepted Answer

A)  transfer RNA 
B)  membrane lipids 
C)  ribosomal RNA 
D)  DNA 
E)  proteins 
A)  transfer RNA 
B)  membrane lipids 
C)  ribosomal RNA 
D)  DNA 
E)  proteins

Question 17

Three-dimensional images of specimens can be obtained using scanning electron microscopes.

Accepted Answer

A) True 
 B)False

Question 18

The Gram stain can be used to distinguish bacteria based on the structure of their cell walls.

Accepted Answer

A) True 
 B)False

Question 19

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

Accepted Answer

Light rays can be manipulated to increas

Question 20

Gram staining of bacteria provides all the physical characterization necessary to identify bacterial species.

Accepted Answer

A) True 
 B)False

Exam 4: Microscopy, Staining, and Classification

A tick is usually measured in terms of

All of the following are common to both the Gram stain and the acid-fast stain EXCEPT

In a compound microscope, the lens that directs light through the specimen is the __________ lens.

The most appropriate unit of measurement for intact archaea is the

Which of the following is NOT associated with an electron microscope?

In Gram staining, ethanol-acetone is used as a

Compare and contrast the three domains identified by Woese: Eukarya, Bacteria, and Archaea.

If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result?

Which of the following phenomena produces magnification?

The dimensions of a virus are usually described in

A patient suffering from tuberculosis could be diagnosed by use of the __________ stain.

Which of the following is NOT a characteristic of a genus name?

Why does immersion oil improve resolution?

Carbolfuchsin is the __________ in the acid-fast stain.

A structure that appears in a transmission electron micrograph but is not actually present in the specimen is known as a(n)

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Three-dimensional images of specimens can be obtained using scanning electron microscopes.

The Gram stain can be used to distinguish bacteria based on the structure of their cell walls.

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

Gram staining of bacteria provides all the physical characterization necessary to identify bacterial species.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant Dna Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Aids and Other Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Environmental Microbiology

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