Question 1

When making slides of thin materials such as urine and cerebrospinal fluid (CSF)

Accepted Answer

A)  spread the material once, let the specimen dry, then spread another layer on top to ensure enough specimen to stain. 
B)  use the two-slide pull method to ensure evenly distributed material over the slides. 
C)  dip a swab into the specimen, then roll the swab over the glass slide to ensure adequate coverage for staining. 
D)  mark the area of the sample drop on the reverse side with a wax pencil and then place a drop of the specimen into the marked area. 
A)  spread the material once, let the specimen dry, then spread another layer on top to ensure enough specimen to stain. 
B)  use the two-slide pull method to ensure evenly distributed material over the slides. 
C)  dip a swab into the specimen, then roll the swab over the glass slide to ensure adequate coverage for staining. 
D)  mark the area of the sample drop on the reverse side with a wax pencil and then place a drop of the specimen into the marked area.

Question 2

A cytocentrifuge is an excellent method for preparing nonviscous fluids, because

Accepted Answer

A)  it deposits cellular elements and microorganisms from the specimen onto the surface of a glass slide as a monolayer. 
B)  it concentrates the protein evenly throughout the slide surface. 
C)  the sediment is mixed with 70% albumin before spreading it on the surface of a slide. 
D)  the cytocentrifuge not only spreads the specimen over the surface of a slide but also stains the specimen. 
A)  it deposits cellular elements and microorganisms from the specimen onto the surface of a glass slide as a monolayer. 
B)  it concentrates the protein evenly throughout the slide surface. 
C)  the sediment is mixed with 70% albumin before spreading it on the surface of a slide. 
D)  the cytocentrifuge not only spreads the specimen over the surface of a slide but also stains the specimen.

Question 3

The most bothersome contaminating materials for the laboratory professional are those that

Accepted Answer

A)  make it hard to see the pathogenic bacteria through all the mucus. 
B)  contain microorganisms that will grow in culture and confuse the culture evaluation. 
C)  make processing the specimen through digestion and straining extremely time consuming. 
D)  appear to resemble fungus on the direct smear. 
A)  make it hard to see the pathogenic bacteria through all the mucus. 
B)  contain microorganisms that will grow in culture and confuse the culture evaluation. 
C)  make processing the specimen through digestion and straining extremely time consuming. 
D)  appear to resemble fungus on the direct smear.

Question 4

If a symptomatic patient presents to a physician with an infection, the physician will treat the patient with antibiotics. What role does the laboratory play in antibiotic selection if the physician has already treated the patient before receiving the culture results?

Accepted Answer

A)  The physician has only given the patient antibiotic samples, and they will only last for a couple days-until the culture results are ready and a proper prescription can be written. 
B)  Physicians will only write antibiotic prescriptions for patients who are infected with a large number of bacteria, so the laboratory needs to tell the physician which antibiotic to use to treat the patient infected with a small number of bacteria. 
C)  The physician can treat the patient for bacterial infections, but if the patient has a fungal infection, the physician relies on the laboratory to tell her or him if the patient has a fungal infection. 
D)  The physician expects the laboratory to affirm or reject the antibiotic choice made after the presumptive diagnosis. 
A)  The physician has only given the patient antibiotic samples, and they will only last for a couple days-until the culture results are ready and a proper prescription can be written. 
B)  Physicians will only write antibiotic prescriptions for patients who are infected with a large number of bacteria, so the laboratory needs to tell the physician which antibiotic to use to treat the patient infected with a small number of bacteria. 
C)  The physician can treat the patient for bacterial infections, but if the patient has a fungal infection, the physician relies on the laboratory to tell her or him if the patient has a fungal infection. 
D)  The physician expects the laboratory to affirm or reject the antibiotic choice made after the presumptive diagnosis.

Exam 7: Microscopic Examination of Materials From Infected Sites

When making slides of thin materials such as urine and cerebrospinal fluid (CSF)

A cytocentrifuge is an excellent method for preparing nonviscous fluids, because

The most bothersome contaminating materials for the laboratory professional are those that

If a symptomatic patient presents to a physician with an infection, the physician will treat the patient with antibiotics. What role does the laboratory play in antibiotic selection if the physician has already treated the patient before receiving the culture results?

Bacterial Cell Structure, Physiology, Metabolism, and Genetics

Hostparasite Interaction

The Laboratory Role in Infection Control

Control of Microorganisms

Performance Improvement in the Microbiology Laboratory

Specimen Collection and Processing

Use of Colony Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

Applications of Molecular Diagnostics

Antimicrobial Agent Mechanisms of Action and Resistance Mechanisms

Antimicrobial Susceptibility Testing

Staphylococci

Streptococcus, Enterococcus, and Other Catalase-Negative, Gram-Positive Cocci

Aerobic Gram-Positive Bacilli

Neisseria Species and Moraxella Catarrhalis

Haemophilus, Hacek, Legionella, and Other Fastidious Gram-Negative Bacilli

Enterobacteriaceae

Vibrio, Aeromonas, and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

Anaerobes of Clinical Importance

The Spirochetes

Chlamydia, Rickettsia, and Similar Organisms

Mycoplasma and Ureaplasma

Mycobacterium Tuberculosis and Nontuberculous Mycobacteria

Medically Significant Fungi

Diagnostic Parasitology

Clinical Virology

Agents of Bioterror and Forensic Microbiology

Biofilms: Architects of Disease

Upper and Lower Respiratory Tract Infections

Skin and Soft Tissue Infections

Gastrointestinal Infections and Food Poisoning

Infections of the Central Nervous System

Bacteremia and Sepsis

Urinary Tract Infections

Genital Infections and Sexually Transmitted Infections

Infections in Special Populations

Zoonotic Diseases

Ocular Infections

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