Question 1

Which microscope uses two beams of light to produce a three-dimensional color image?

Accepted Answer

A)  phase-contrast microscope 
B)  DIC microscope 
C)  electron microscope 
D)  fluorescence microscope 
E)  darkfield microscope 
A)  phase-contrast microscope 
B)  DIC microscope 
C)  electron microscope 
D)  fluorescence microscope 
E)  darkfield microscope

Question 2

What structure does light pass through after leaving the condenser in a compound light microscope?

Accepted Answer

A)  illuminator 
B)  objective lens 
C)  specimen 
D)  ocular lens 
A)  illuminator 
B)  objective lens 
C)  specimen 
D)  ocular lens

Question 3

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

Accepted Answer

A)  an acid-fast stain 
B)  a flagella stain 
C)  an endospore stain 
D)  a capsule stain 
E)  a simple stain 
A)  an acid-fast stain 
B)  a flagella stain 
C)  an endospore stain 
D)  a capsule stain 
E)  a simple stain

Question 4

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point?

Accepted Answer

A)  purple 
B)  red 
C)  colorless 
D)  brown 
A)  purple 
B)  red 
C)  colorless 
D)  brown

Question 5

Which of the following is NOT equal to 1 mm?

Accepted Answer

A)  100 μm 
B)  10-3 m 
C)  106 nm 
D)  0.1 cm 
E)  0.001 m 
A)  100 μm 
B)  10-3 m 
C)  106 nm 
D)  0.1 cm 
E)  0.001 m

Question 6

In 1884, Hans Christian Gram described a method of staining bacterial cells while not staining surrounding animal tissues. However, he thought that the staining method he developed was faulty because not all bacteria stained. In a letter to the editor of the journal in which Gram published his findings, write your response to Gramʹs concern.

Accepted Answer

The answer of In 1884, Hans Christian Gram described a...

Question 7

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet
3-Safranin 4-Iodine

Accepted Answer

A)  1-3-2-4 
B)  1-2-3-4 
C)  2-1-4-3 
D)  2-4-1-3 
E)  4-3-2-1 
A)  1-3-2-4 
B)  1-2-3-4 
C)  2-1-4-3 
D)  2-4-1-3 
E)  4-3-2-1

Question 8

A microorganism measures 5 μm in length. Its length in mm would be

Accepted Answer

A)  500 mm. 
B)  0.5 mm. 
C)  0.005 mm. 
D)  0.05 mm. 
E)  50 mm. 
A)  500 mm. 
B)  0.5 mm. 
C)  0.005 mm. 
D)  0.05 mm. 
E)  50 mm.

Question 9

In a completed Gram stain, gram-negative bacteria are colorless.

Accepted Answer

A) True 
 B)False

Question 10

The counterstain used in the Gram stain is a basic dye.

Accepted Answer

A) True 
 B)False

Question 11

In a completed Gram stain, gram-positive bacteria are purple.

Accepted Answer

A) True 
 B)False

Question 12

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Accepted Answer

A) True 
 B)False

Question 13

Which microscope achieves the highest magnification and greatest resolution?

Accepted Answer

A)  darkfield microscope 
B)  electron microscope 
C)  compound light microscope 
D)  phase-contrast microscope 
E)  fluorescence microscope 
A)  darkfield microscope 
B)  electron microscope 
C)  compound light microscope 
D)  phase-contrast microscope 
E)  fluorescence microscope

Question 14

Which microscope is used to see intracellular detail in a living cell?

Accepted Answer

A)  fluorescence microscope 
B)  transmission electron microscope 
C)  brightfield microscope 
D)  atomic force microscope 
E)  two-photon microscope 
A)  fluorescence microscope 
B)  transmission electron microscope 
C)  brightfield microscope 
D)  atomic force microscope 
E)  two-photon microscope

Question 15

Simple staining is often necessary to improve contrast in which microscope?

Accepted Answer

A)  fluorescence microscope 
B)  phase-contrast microscope 
C)  compound light microscope 
D)  darkfield microscope 
E)  electron microscope 
A)  fluorescence microscope 
B)  phase-contrast microscope 
C)  compound light microscope 
D)  darkfield microscope 
E)  electron microscope

Question 16

Bacterial smears are fixed before staining to

Accepted Answer

A)  accept stain. 
B)  affix the cells to the slide. 
C)  make the cells visible. 
D)  make their walls permeable. 
A)  accept stain. 
B)  affix the cells to the slide. 
C)  make the cells visible. 
D)  make their walls permeable.

Question 17

Which microscope can be used to visualize DNA or botulinum toxin?

Accepted Answer

A)  compound light microscope 
B)  phase-contrast microscope 
C)  scanning tunneling microscope 
D)  scanning electron microscope 
E)  confocal microscope 
A)  compound light microscope 
B)  phase-contrast microscope 
C)  scanning tunneling microscope 
D)  scanning electron microscope 
E)  confocal microscope

Question 18

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Accepted Answer

A)  brightfield microscope 
B)  phase-contrast microscope 
C)  scanning electron microscope 
D)  darkfield microscope 
E)  transmission electron microscope 
A)  brightfield microscope 
B)  phase-contrast microscope 
C)  scanning electron microscope 
D)  darkfield microscope 
E)  transmission electron microscope

Question 19

Which of the following is never useful for observing living cells?

Accepted Answer

A)  scanning electron microscope 
B)  phase-contrast microscope 
C)  scanning acoustic microscope 
D)  brightfield microscope 
E)  darkfield microscope 
A)  scanning electron microscope 
B)  phase-contrast microscope 
C)  scanning acoustic microscope 
D)  brightfield microscope 
E)  darkfield microscope

Question 20

Which microscope uses visible light?

Accepted Answer

A)  confocal microscope 
B)  differential interference contrast microscope 
C)  scanning acoustic microscope 
D)  scanning electron microscope 
E)  fluorescence microscope 
A)  confocal microscope 
B)  differential interference contrast microscope 
C)  scanning acoustic microscope 
D)  scanning electron microscope 
E)  fluorescence microscope

Exam 3: Observing Microorganisms Through a Microscope

Which microscope uses two beams of light to produce a three-dimensional color image?

What structure does light pass through after leaving the condenser in a compound light microscope?

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point?

Which of the following is NOT equal to 1 mm?

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-Iodine

A microorganism measures 5 μm in length. Its length in mm would be

In a completed Gram stain, gram-negative bacteria are colorless.

The counterstain used in the Gram stain is a basic dye.

In a completed Gram stain, gram-positive bacteria are purple.

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Which microscope achieves the highest magnification and greatest resolution?

Which microscope is used to see intracellular detail in a living cell?

Simple staining is often necessary to improve contrast in which microscope?

Bacterial smears are fixed before staining to

Which microscope can be used to visualize DNA or botulinum toxin?

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Which of the following is never useful for observing living cells?

Which microscope uses visible light?

The Microbial World and You

Chemical Principles

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Biotechnology and Dna Technology

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Disease of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Filters