Exam 9: Biotechnology and Dna Technology

The following steps must be performed to make a bacterium produce human protein X. 1- Translation 2- Restriction enzyme 3- Prokaryotic transcription 4-DNA ligase 5- Transformation 6- Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction.

A population of cells carrying a desired plasmid is called a

Nearly all cells, including E. coli and yeast, naturally take up DNA from their surroundings without chemical treatment.

Biotechnology involves the

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

An advantage of cDNA over genomic DNA is that it

Figure 9.5 -In Figure 9.5, the gene that allows the plasmid to be self-replicating is

The random shotgun method is used in

The study of genetic material taken directly from the environment is

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Restriction enzymes are

The use of ʺsuicideʺ genes in genetically modified organisms is designed to

A restriction fragment is

Which of the following places the steps in the PCR procedure in the correct order? 1) Incubate at 94°C to denature DNA strands; 2) Incubate at 72°C for DNA synthesis; 3) Incubate at 60°C for primer hybridization.

The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells. A new, rapid diagnostic test to detect human papilloma virus (HPV) DNA before cancer develops is done without microscopic exam. The steps involved in this FastHPV test are listed below. What is the second step?

PCR can be used to identify an unknown bacterium because

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

The local public health agency has received reports of an outbreak of Salmonella gastroenteritis among attendees at a city-sponsored chili cook-off. What techniques from recombinant DNA technology would the agency likely use to investigate this outbreak? Describe the expected results from these techniques.