Question 1

The counterstain in the acid- fast stain is

Accepted Answer

A)  A mordant. 
B)  A negative stain. 
C)  A basic dye. 
D)  An acid dye. 
E)  Necessary to determine acid- fast cells. 
A)  A mordant. 
B)  A negative stain. 
C)  A basic dye. 
D)  An acid dye. 
E)  Necessary to determine acid- fast cells. C

Question 2

The appearance of gram- negative bacteria after addition of the mordant in the Gram stain.

Accepted Answer

A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above 
A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above A

Question 3

This microscope is used to see internal structures of cells in a natural state.

Accepted Answer

A)  Compound light microscope 
B)  Phase- contrast microscope 
C)  Darkfield microscope 
D)  Fluorescence microscope 
E)  Electron microscope 
A)  Compound light microscope 
B)  Phase- contrast microscope 
C)  Darkfield microscope 
D)  Fluorescence microscope 
E)  Electron microscope B

Question 4

The purpose of a mordant in the Gram stain is

Accepted Answer

A)  To prevent the crystal violet from leaving the cells. 
B)  To make the bacterial cells larger. 
C)  To make the flagella visible. 
D)  To remove the simple stain. 
E)  None of the above. 
A)  To prevent the crystal violet from leaving the cells. 
B)  To make the bacterial cells larger. 
C)  To make the flagella visible. 
D)  To remove the simple stain. 
E)  None of the above.

Question 5

In this microscope, the observer does not look at an image through a lens.

Accepted Answer

A)  Compound light microscope 
B)  Phase- contrast microscope 
C)  Darkfield microscope 
D)  Fluorescence microscope 
E)  Electron microscope 
A)  Compound light microscope 
B)  Phase- contrast microscope 
C)  Darkfield microscope 
D)  Fluorescence microscope 
E)  Electron microscope

Question 6

Which of the following is not correct?

Accepted Answer

A)  1 µm = 10- 3 mm 
B)  1 nm = 10- 6 µm 
C)  1 µm = 10- 6 m 
D)  1 µm = 103 nm 
E)  1 nm = 10- 9 m 
A)  1 µm = 10- 3 mm 
B)  1 nm = 10- 6 µm 
C)  1 µm = 10- 6 m 
D)  1 µm = 103 nm 
E)  1 nm = 10- 9 m

Question 7

The signal molecule produced in quorum sensing is

Accepted Answer

A)  Simple stain. 
B)  Counterstain. 
C)  Inducer. 
D)  Light. 
E)  None of the above. 
A)  Simple stain. 
B)  Counterstain. 
C)  Inducer. 
D)  Light. 
E)  None of the above.

Question 8

The appearance of gram- positive bacteria after adding the counterstain in the Gram stain.

Accepted Answer

A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above 
A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above

Question 9

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

Accepted Answer

A)  Capsules. 
B)  Flagella. 
C)  Cell walls. 
D)  Endospores. 
E)  Can't tell. 
A)  Capsules. 
B)  Flagella. 
C)  Cell walls. 
D)  Endospores. 
E)  Can't tell.

Question 10

The counterstain in the Gram stain is

Accepted Answer

A)  A mordant. 
B)  An acid dye. 
C)  A negative stain. 
D)  A basic dye. 
E)  Necessary to determine the Gram reaction. 
A)  A mordant. 
B)  An acid dye. 
C)  A negative stain. 
D)  A basic dye. 
E)  Necessary to determine the Gram reaction.

Question 11

The purpose of the ocular lens is to

Accepted Answer

A)  Increase the light. 
B)  Magnify the image from the objective lens. 
C)  Improve resolution. 
D)  Decrease the refractive index. 
E)  None of the above. 
A)  Increase the light. 
B)  Magnify the image from the objective lens. 
C)  Improve resolution. 
D)  Decrease the refractive index. 
E)  None of the above.

Question 12

This microscope uses two beams of light to produce a three- dimensional, color image.

Accepted Answer

A)  DIC microscope 
B)  Darkfield microscope 
C)  Fluorescence microscope 
D)  Phase- contrast microscope 
E)  None of the above 
A)  DIC microscope 
B)  Darkfield microscope 
C)  Fluorescence microscope 
D)  Phase- contrast microscope 
E)  None of the above

Question 13

Figure 3.1
  
-In Figure 3.1 line B points to the microscope's

Accepted Answer

A)  ocular lens. 
B)  condenser. 
C)  illuminator. 
D)  objective lens. 
A)  ocular lens. 
B)  condenser. 
C)  illuminator. 
D)  objective lens.

Question 14

Figure 3.1
  
-In Figure 3.1 line A points to the microscope's

Accepted Answer

A)  condenser. 
B)  objective lens. 
C)  ocular lens. 
D)  illuminator. 
A)  condenser. 
B)  objective lens. 
C)  ocular lens. 
D)  illuminator.

Question 15

The best use of a negative stain is

Accepted Answer

A)  To determine cell size. 
B)  To determine cell shape. 
C)  To see endospores. 
D)  To determine Gram reaction. 
E)  A and B 
A)  To determine cell size. 
B)  To determine cell shape. 
C)  To see endospores. 
D)  To determine Gram reaction. 
E)  A and B

Question 16

The appearance of gram- positive bacteria after addition of the first dye in the Gram stain.

Accepted Answer

A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above 
A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above

Question 17

Cells are differentiated after which step in the Gram stain?

Accepted Answer

A)  Alcohol- acetone 
B)  Crystal violet 
C)  Iodine 
D)  Safranin 
A)  Alcohol- acetone 
B)  Crystal violet 
C)  Iodine 
D)  Safranin

Question 18

Simple staining is often necessary to improve contrast in this microscope.

Accepted Answer

A)  Compound light microscope 
B)  Phase- contrast microscope 
C)  Darkfield microscope 
D)  Fluorescence microscope 
E)  Electron microscope 
A)  Compound light microscope 
B)  Phase- contrast microscope 
C)  Darkfield microscope 
D)  Fluorescence microscope 
E)  Electron microscope

Question 19

The appearance of gram- negative bacteria after addition of the decolorizing agent in the Gram stain.

Accepted Answer

A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above 
A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above

Question 20

The appearance of gram- negative bacteria after completing the Gram stain.

Accepted Answer

A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above 
A)  Purple 
B)  Red 
C)  Colorless 
D)  Brown 
E)  None of the above

Exam 3: Observing Microorganisms Through a Microscope

The counterstain in the acid- fast stain is

The appearance of gram- negative bacteria after addition of the mordant in the Gram stain.

This microscope is used to see internal structures of cells in a natural state.

The purpose of a mordant in the Gram stain is

In this microscope, the observer does not look at an image through a lens.

Which of the following is not correct?

The signal molecule produced in quorum sensing is

The appearance of gram- positive bacteria after adding the counterstain in the Gram stain.

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

The counterstain in the Gram stain is

The purpose of the ocular lens is to

This microscope uses two beams of light to produce a three- dimensional, color image.

Figure 3.1 -In Figure 3.1 line B points to the microscope's

Figure 3.1 -In Figure 3.1 line A points to the microscope's

The best use of a negative stain is

The appearance of gram- positive bacteria after addition of the first dye in the Gram stain.

Cells are differentiated after which step in the Gram stain?

Simple staining is often necessary to improve contrast in this microscope.

The appearance of gram- negative bacteria after addition of the decolorizing agent in the Gram stain.

The appearance of gram- negative bacteria after completing the Gram stain.

The Microbial World and You

Chemical Principles

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Biotechnology and Recombinant DNA

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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