Question 1

Subunit vaccines can be made by genetic modification of yeast cells. A side effect of these vaccines might be

Accepted Answer

A)  Due to extraneous material. 
B)  A yeast infection. 
C)  That the vaccine doesn't provide immunity. 
D)  The disease. 
E)  None of the above. 
A)  Due to extraneous material. 
B)  A yeast infection. 
C)  That the vaccine doesn't provide immunity. 
D)  The disease. 
E)  None of the above. E

Question 2

E. coli makes insulin because

Accepted Answer

A)  The insulin gene was inserted into it. 
B)  It picked up the insulin gene from another cell. 
C)  It's an ancient gene that now has no function. 
D)  It needs to regulate its cell- glucose level. 
E)  No reason; it doesn't make insulin. 
A)  The insulin gene was inserted into it. 
B)  It picked up the insulin gene from another cell. 
C)  It's an ancient gene that now has no function. 
D)  It needs to regulate its cell- glucose level. 
E)  No reason; it doesn't make insulin. A

Question 3

Figure 9.1
  
-How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

Accepted Answer

A)  2 
B)  1 
C)  4 
D)  5 
E)  3 
A)  2 
B)  1 
C)  4 
D)  5 
E)  3 A

Question 4

The value of cDNA in recombinant DNA is that

Accepted Answer

A)  It lacks introns. 
B)  It lacks exons. 
C)  It's really RNA. 
D)  None of the above. 
A)  It lacks introns. 
B)  It lacks exons. 
C)  It's really RNA. 
D)  None of the above.

Question 5

The following steps are used to make DNA fingerprints. What is the third step?

Accepted Answer

A)  Add stain. 
B)  Perform electrophoresis. 
C)  Collect DNA. 
D)  Digest with a restriction enzyme. 
E)  Lyse cells. 
A)  Add stain. 
B)  Perform electrophoresis. 
C)  Collect DNA. 
D)  Digest with a restriction enzyme. 
E)  Lyse cells.

Question 6

A population of cells carrying a desired plasmid is called a

Accepted Answer

A)  Library. 
B)  Southern blot. 
C)  Clone. 
D)  PCR. 
E)  Vector. 
A)  Library. 
B)  Southern blot. 
C)  Clone. 
D)  PCR. 
E)  Vector.

Question 7

An example of gene therapy is

Accepted Answer

A)  Insertion of the insulin gene in a diabetic person's pancreas cells. 
B)  Insertion of the insulin gene in a mammalian cell culture. 
C)  Insertion of the insulin gene in E. coli. 
D)  Injection of insulin into a diabetic person. 
E)  None of the above. 
A)  Insertion of the insulin gene in a diabetic person's pancreas cells. 
B)  Insertion of the insulin gene in a mammalian cell culture. 
C)  Insertion of the insulin gene in E. coli. 
D)  Injection of insulin into a diabetic person. 
E)  None of the above.

Question 8

Figure 9.3
  
-In Figure 9.3, the resulting P. fluorescens has

Accepted Answer

A)  An E. coli gene. 
B)  A tomato and a Bacillus gene. 
C)  A Bacillus gene. 
D)  A tomato gene. 
E)  No new gene. 
A)  An E. coli gene. 
B)  A tomato and a Bacillus gene. 
C)  A Bacillus gene. 
D)  A tomato gene. 
E)  No new gene.

Question 9

Pieces of DNA stored in yeast cells are called a

Accepted Answer

A)  Library. 
B)  Southern blot. 
C)  Clone. 
D)  Vector. 
E)  PCR. 
A)  Library. 
B)  Southern blot. 
C)  Clone. 
D)  Vector. 
E)  PCR.

Question 10

Which of the following techniques is used to alter one amino acid in protein?

Accepted Answer

A)  Cloning 
B)  Restriction enzymes 
C)  PCR 
D)  Selection 
E)  Site- direct mutagenesis 
A)  Cloning 
B)  Restriction enzymes 
C)  PCR 
D)  Selection 
E)  Site- direct mutagenesis

Question 11

Figure 9.3
  
-In Figure 9.3, the process required in step 5 is

Accepted Answer

A)  PCR. 
B)  Southern blotting. 
C)  Transformation. 
D)  None of the above. 
A)  PCR. 
B)  Southern blotting. 
C)  Transformation. 
D)  None of the above.

Question 12

Self- replicating DNA used to transmit a gene from one organism to another is a

Accepted Answer

A)  Library. 
B)  Vector. 
C)  Clone. 
D)  Southern blot. 
E)  PCR. 
A)  Library. 
B)  Vector. 
C)  Clone. 
D)  Southern blot. 
E)  PCR.

Question 13

A specific gene can be inserted into a cell by all of the following except

Accepted Answer

A)  Microinjection. 
B)  Electroporation. 
C)  Agrobacterium. 
D)  A gene gun. 
E)  Protoplast fusion. 
A)  Microinjection. 
B)  Electroporation. 
C)  Agrobacterium. 
D)  A gene gun. 
E)  Protoplast fusion.

Question 14

Which of the following methods of making rDNA could be described as "hit or miss"?

Accepted Answer

A)  Cloning 
B)  Transformation 
C)  Viral transduction 
D)  Protoplast fusion 
E)  None of the above 
A)  Cloning 
B)  Transformation 
C)  Viral transduction 
D)  Protoplast fusion 
E)  None of the above

Question 15

A technique used to identify bacteria carrying a specific gene is

Accepted Answer

A)  Shotgun sequencing. 
B)  Transformation. 
C)  Southern blot. 
D)  Cloning. 
E)  None of the above. 
A)  Shotgun sequencing. 
B)  Transformation. 
C)  Southern blot. 
D)  Cloning. 
E)  None of the above.

Question 16

You have a small gene that you wish replicated by PCR. You add radioactively labeled nucleotides to the PCR machine. After 3 replication cycles, how many double- stranded DNA molecules do you have?

Accepted Answer

A)  2 
B)  Thousands 
C)  16 
D)  8 
E)  4 
A)  2 
B)  Thousands 
C)  16 
D)  8 
E)  4

Question 17

Restriction enzymes are

Accepted Answer

A)  Animal enzymes that splice RNA. 
B)  Viral enzymes that destroy host DNA. 
C)  Bacterial enzymes that destroy phage DNA. 
D)  Bacterial enzymes that splice DNA. 
A)  Animal enzymes that splice RNA. 
B)  Viral enzymes that destroy host DNA. 
C)  Bacterial enzymes that destroy phage DNA. 
D)  Bacterial enzymes that splice DNA.

Question 18

A restriction fragment is

Accepted Answer

A)  A segment of RNA. 
B)  A gene. 
C)  A segment of DNA. 
D)  None of the above. 
A)  A segment of RNA. 
B)  A gene. 
C)  A segment of DNA. 
D)  None of the above.

Question 19

Figure 9.3
  
-In Figure 9.3, the vector is

Accepted Answer

A)  A library. 
B)  A plasmid. 
C)  A virus. 
D)  None of the above. 
A)  A library. 
B)  A plasmid. 
C)  A virus. 
D)  None of the above.

Question 20

A eukaryote used in genetic engineering is

Accepted Answer

A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Thermus aquaticus. 
D)  Agrobacterium tumefaciens. 
E)  Bacillus thuringiensis. 
A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Thermus aquaticus. 
D)  Agrobacterium tumefaciens. 
E)  Bacillus thuringiensis.

Exam 9: Biotechnology and Recombinant DNA

Subunit vaccines can be made by genetic modification of yeast cells. A side effect of these vaccines might be

E. coli makes insulin because

Figure 9.1 -How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

The value of cDNA in recombinant DNA is that

The following steps are used to make DNA fingerprints. What is the third step?

A population of cells carrying a desired plasmid is called a

An example of gene therapy is

Figure 9.3 -In Figure 9.3, the resulting P. fluorescens has

Pieces of DNA stored in yeast cells are called a

Which of the following techniques is used to alter one amino acid in protein?

Figure 9.3 -In Figure 9.3, the process required in step 5 is

Self- replicating DNA used to transmit a gene from one organism to another is a

A specific gene can be inserted into a cell by all of the following except

Which of the following methods of making rDNA could be described as "hit or miss"?

A technique used to identify bacteria carrying a specific gene is

You have a small gene that you wish replicated by PCR. You add radioactively labeled nucleotides to the PCR machine. After 3 replication cycles, how many double- stranded DNA molecules do you have?

Restriction enzymes are

A restriction fragment is

Figure 9.3 -In Figure 9.3, the vector is

A eukaryote used in genetic engineering is

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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