Question 1

The total magnification of a compound microscope is calculated using the magnification of which of the following?

Accepted Answer

A)  the objective lens and the prism 
B)  the condenser and the ocular lenses 
C)  the condenser lens and the diaphragm 
D)  the objective and the ocular lenses 
E)  the objective and the condenser lense 
A)  the objective lens and the prism 
B)  the condenser and the ocular lenses 
C)  the condenser lens and the diaphragm 
D)  the objective and the ocular lenses 
E)  the objective and the condenser lense D

Question 2

Carbolfuchsin is used in which of the following staining procedures?

Accepted Answer

A)  endospore stain 
B)  capsule stain 
C)  Gram stain 
D)  flagellar stain 
E)  acid- fast stain 
A)  endospore stain 
B)  capsule stain 
C)  Gram stain 
D)  flagellar stain 
E)  acid- fast stain E

Question 3

In taxonomy, organisms in similar classes can be classified in larger groups called _.

Accepted Answer

phyla

Question 4

A structure that appears in a transmission electron micrograph but which is not actually present in the specimen is known as a(n)

Accepted Answer

A)  mordant. 
B)  biofilm. 
C)  artifact. 
D)  refraction. 
E)  antigen. 
A)  mordant. 
B)  biofilm. 
C)  artifact. 
D)  refraction. 
E)  antigen.

Question 5

Which of the following types of microscopes is capable of generating a three- dimensional image of a specimen?

Accepted Answer

A)  scanning electron 
B)  differential interference contrast 
C)  confocal 
D)  Both B and C are correct. 
E)  A, B, and C are correct. 
A)  scanning electron 
B)  differential interference contrast 
C)  confocal 
D)  Both B and C are correct. 
E)  A, B, and C are correct.

Question 6

Light rays that are in phase reinforce each other and produce a brighter image.

Accepted Answer

A) True 
 B)False

Question 7

Why does immersion oil improve resolution?

Accepted Answer

A)  It increases the numerical aperture. 
B)  It decreases the working distance. 
C)  It allows light to travel at a uniform speed on its way to the lens. 
D)  Both A and C are correct. 
E)  A, B, and C are correct. 
A)  It increases the numerical aperture. 
B)  It decreases the working distance. 
C)  It allows light to travel at a uniform speed on its way to the lens. 
D)  Both A and C are correct. 
E)  A, B, and C are correct.

Question 8

The numerical aperture of a lens refers to its

Accepted Answer

A)  ability to gather light. 
B)  working distance. 
C)  resolution distance. 
D)  curvature. 
E)  thickness. 
A)  ability to gather light. 
B)  working distance. 
C)  resolution distance. 
D)  curvature. 
E)  thickness.

Question 9

In the Linnaean system of taxonomy, an order is a subset of a larger taxonomic group called the

Accepted Answer

A)  class. 
B)  kingdom. 
C)  phylum. 
D)  family. 
E)  genus. 
A)  class. 
B)  kingdom. 
C)  phylum. 
D)  family. 
E)  genus.

Question 10

Bacterial cells are so similar to each other that physical characteristics cannot be used in identification of organisms.

Accepted Answer

A) True 
 B)False

Question 11

List and explain five types of techniques that can be used to identify unknown microorganisms.

Accepted Answer

There are five major types of identifica

Question 12

All of the following are characteristics of a genus name EXCEPT:

Accepted Answer

A)  it is one of two names used to identify an organism. 
B)  it is either underlined or in italics. 
C)  it is usually an adjective. 
D)  it is written before the specific epithet. 
E)  it is always capitalized. 
A)  it is one of two names used to identify an organism. 
B)  it is either underlined or in italics. 
C)  it is usually an adjective. 
D)  it is written before the specific epithet. 
E)  it is always capitalized.

Question 13

An agglutination test is an example of a(n) type of classification test.

Accepted Answer

The answer of An agglutination test is an example of...

Question 14

All of the following are components of electron microscopy stains EXCEPT:

Accepted Answer

A)  lead. 
B)  eosin. 
C)  tungsten. 
D)  osmium. 
E)  uranium. 
A)  lead. 
B)  eosin. 
C)  tungsten. 
D)  osmium. 
E)  uranium.

Question 15

Which of the following is NOT true of transmission electron microscopy?

Accepted Answer

A)  Electrons are the source of illumination. 
B)  Thin segments must be used. 
C)  The sample is placed in a vacuum. 
D)  Glass lenses are used. 
E)  Stains can be applied to improve contrast. 
A)  Electrons are the source of illumination. 
B)  Thin segments must be used. 
C)  The sample is placed in a vacuum. 
D)  Glass lenses are used. 
E)  Stains can be applied to improve contrast.

Question 16

The refraction of radiation as it passes through a lens creates which of the following?

Accepted Answer

A)  resolving power 
B)  magnification 
C)  immunofluorescence 
D)  resolution distance 
E)  contrast 
A)  resolving power 
B)  magnification 
C)  immunofluorescence 
D)  resolution distance 
E)  contrast

Question 17

Which of the following microscopes is capable of more than 100,000,000X magnification?

Accepted Answer

A)  bright- field microscopes 
B)  scanning electron microscopes 
C)  differential interference contrast microscopes 
D)  scanning tunneling microscopes 
E)  transmission electron microscopes 
A)  bright- field microscopes 
B)  scanning electron microscopes 
C)  differential interference contrast microscopes 
D)  scanning tunneling microscopes 
E)  transmission electron microscopes

Question 18

A(n) _ molecule is one that absorbs invisible radiation and emits visible light.

Accepted Answer

The answer of A(n) _ molecule is one that absorbs...

Question 19

Acid- fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following staining procedures uses heat to drive the stain into the cell?

Accepted Answer

A)  acid- fast stain 
B)  endospore stain 
C)  capsule stain 
D)  Both A and B are correct. 
E)  A, B, and C are correct. 
A)  acid- fast stain 
B)  endospore stain 
C)  capsule stain 
D)  Both A and B are correct. 
E)  A, B, and C are correct.

Exam 4: Microscopy, Staining, and Classification

The total magnification of a compound microscope is calculated using the magnification of which of the following?

Carbolfuchsin is used in which of the following staining procedures?

In taxonomy, organisms in similar classes can be classified in larger groups called _.

A structure that appears in a transmission electron micrograph but which is not actually present in the specimen is known as a(n)

Which of the following types of microscopes is capable of generating a three- dimensional image of a specimen?

Light rays that are in phase reinforce each other and produce a brighter image.

Why does immersion oil improve resolution?

The numerical aperture of a lens refers to its

In the Linnaean system of taxonomy, an order is a subset of a larger taxonomic group called the

Bacterial cells are so similar to each other that physical characteristics cannot be used in identification of organisms.

List and explain five types of techniques that can be used to identify unknown microorganisms.

All of the following are characteristics of a genus name EXCEPT:

An agglutination test is an example of a(n) type of classification test.

All of the following are components of electron microscopy stains EXCEPT:

Which of the following is NOT true of transmission electron microscopy?

The refraction of radiation as it passes through a lens creates which of the following?

Which of the following microscopes is capable of more than 100,000,000X magnification?

A(n) _ molecule is one that absorbs invisible radiation and emits visible light.

Acid- fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid.

Which of the following staining procedures uses heat to drive the stain into the cell?

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant Dna Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Aids and Other Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Environmental Microbiology

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