Question 1

Nitrocellulose membranes used in Southern blotting are stronger than agarose gels.

Accepted Answer

A) True 
 B)False  True

Question 2

A thermocycler is used for which of the following techniques?

Accepted Answer

A)  Southern blotting 
B)  DNA microarrays 
C)  PCR 
D)  protoplast fusion 
E)  electroporation 
A)  Southern blotting 
B)  DNA microarrays 
C)  PCR 
D)  protoplast fusion 
E)  electroporation C

Question 3

Vectors usually contain _ such as antibiotic resistance genes or fluorescent tags.

Accepted Answer

genetic markers

Question 4

All of the following are required for PCR EXCEPT:

Accepted Answer

A)  DNA polymerase. 
B)  fluorescent probes. 
C)  double- stranded DNA molecules. 
D)  deoxyribonucleotides. 
E)  primers. 
A)  DNA polymerase. 
B)  fluorescent probes. 
C)  double- stranded DNA molecules. 
D)  deoxyribonucleotides. 
E)  primers.

Question 5

Which of the following methods of inserting DNA into cells might be used on plant seeds?

Accepted Answer

A)  electroporation 
B)  injection 
C)  protoplast fusion 
D)  Both A and C are correct. 
E)  A, B, and C are correct. 
A)  electroporation 
B)  injection 
C)  protoplast fusion 
D)  Both A and C are correct. 
E)  A, B, and C are correct.

Question 6

Sticky- end fragments generated by EcoRI will ligate to any other sticky- end sequence.

Accepted Answer

A) True 
 B)False

Question 7

Discuss the risks associated with the creation of genetically altered organisms. What sorts of safeguards are used to prevent potential problems caused by these organisms?

Accepted Answer

The major risk associated with genetical

Question 8

An antigen is

Accepted Answer

A)  a foreign substance that stimulates immunity. 
B)  a substance used to make gels for DNA electrophoresis. 
C)  a naturally occurring insecticide. 
D)  a genetic marker found in most plasmids. 
E)  a molecule necessary for PCR. 
A)  a foreign substance that stimulates immunity. 
B)  a substance used to make gels for DNA electrophoresis. 
C)  a naturally occurring insecticide. 
D)  a genetic marker found in most plasmids. 
E)  a molecule necessary for PCR.

Question 9

Large DNA molecules such as chromosomes are easier to work with than small molecules.

Accepted Answer

A) True 
 B)False

Question 10

, the biochemical precursor to vitamin A, can be added to rice by using recombinant DNA technology, thereby increasing the nutritional value of the rice.

Accepted Answer

The answer of , the biochemical precursor to vitamin A,...

Question 11

Which of the following microbes produces a protein that kills a variety of insect pests?

Accepted Answer

A)  Plasmodium falciparum 
B)  Bacillus thuringiensis 
C)  Pseudomonas 
D)  Thermus aquaticus 
E)  Haemophilus influenzae 
A)  Plasmodium falciparum 
B)  Bacillus thuringiensis 
C)  Pseudomonas 
D)  Thermus aquaticus 
E)  Haemophilus influenzae

Question 12

A restriction enzyme that cuts both strands of DNA at the same point will produce fragments with .

Accepted Answer

The answer of A restriction enzyme that cuts both strands...

Question 13

The name of a restriction enzyme can contain all the following items of information EXCEPT:

Accepted Answer

A)  Roman numerals to indicate its order of discovery. 
B)  the strain of the source bacterium. 
C)  the specific epithet of the source bacterium. 
D)  the genus of the source bacterium. 
E)  the Gram reaction of the source bacterium. 
A)  Roman numerals to indicate its order of discovery. 
B)  the strain of the source bacterium. 
C)  the specific epithet of the source bacterium. 
D)  the genus of the source bacterium. 
E)  the Gram reaction of the source bacterium.

Question 14

In gel electrophoresis, DNA molecules move toward the _ electrode, because they have an overall
Charge.

Accepted Answer

A)  positive; positive 
B)  negative; negative 
C)  negative; positive 
D)  negative and positive; nonpolar 
E)  positive; negative 
A)  positive; positive 
B)  negative; negative 
C)  negative; positive 
D)  negative and positive; nonpolar 
E)  positive; negative

Question 15

In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make which of the following for the first time in an industrial setting?

Accepted Answer

A)  bread 
B)  acetone 
C)  cheese 
D)  alcohol 
E)  soy sauce 
A)  bread 
B)  acetone 
C)  cheese 
D)  alcohol 
E)  soy sauce

Question 16

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

A) True 
 B)False

Question 17

If a researcher used E. coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Accepted Answer

A)  PCR would occur twice as fast as normal. 
B)  PCR would occur more slowly than normal. 
C)  PCR would not occur at all. 
D)  Many mistakes would occur. 
E)  PCR would stop after one cycle. 
A)  PCR would occur twice as fast as normal. 
B)  PCR would occur more slowly than normal. 
C)  PCR would not occur at all. 
D)  Many mistakes would occur. 
E)  PCR would stop after one cycle.

Question 18

Microarrays are

Accepted Answer

A)  used for disease identification. 
B)  used to look at functional genomics. 
C)  used for sequencing genomes. 
D)  Both A and B are correct. 
E)  A, B, and C are correct. 
A)  used for disease identification. 
B)  used to look at functional genomics. 
C)  used for sequencing genomes. 
D)  Both A and B are correct. 
E)  A, B, and C are correct.

Question 19

A northern blot differs from a Southern blot in

Accepted Answer

A)  the type of probe used. 
B)  the number of genetic sequences detected. 
C)  the size of the genetic sequences involved. 
D)  the presence or absence of a nitrocellulose membrane. 
E)  the type of nucleic acid being isolated. 
A)  the type of probe used. 
B)  the number of genetic sequences detected. 
C)  the size of the genetic sequences involved. 
D)  the presence or absence of a nitrocellulose membrane. 
E)  the type of nucleic acid being isolated.

Question 20

Which of the following restriction enzyme sites would produce blunt- ended fragments (the arrow represents the cutting site of the enzyme)?

Accepted Answer

A)  CCC$GGG 
B)  G$AATTC 
C)  G$GATCC 
D)  A$AGCTT 
E)  C$CGG 
A)  CCC$GGG 
B)  G$AATTC 
C)  G$GATCC 
D)  A$AGCTT 
E)  C$CGG

Exam 8: Recombinant Dna Technology

Nitrocellulose membranes used in Southern blotting are stronger than agarose gels.

A thermocycler is used for which of the following techniques?

Vectors usually contain _ such as antibiotic resistance genes or fluorescent tags.

All of the following are required for PCR EXCEPT:

Which of the following methods of inserting DNA into cells might be used on plant seeds?

Sticky- end fragments generated by EcoRI will ligate to any other sticky- end sequence.

Discuss the risks associated with the creation of genetically altered organisms. What sorts of safeguards are used to prevent potential problems caused by these organisms?

An antigen is

Large DNA molecules such as chromosomes are easier to work with than small molecules.

, the biochemical precursor to vitamin A, can be added to rice by using recombinant DNA technology, thereby increasing the nutritional value of the rice.

Which of the following microbes produces a protein that kills a variety of insect pests?

A restriction enzyme that cuts both strands of DNA at the same point will produce fragments with .

The name of a restriction enzyme can contain all the following items of information EXCEPT:

In gel electrophoresis, DNA molecules move toward the _ electrode, because they have an overall Charge.

In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make which of the following for the first time in an industrial setting?

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

If a researcher used E. coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Microarrays are

A northern blot differs from a Southern blot in

Which of the following restriction enzyme sites would produce blunt- ended fragments (the arrow represents the cutting site of the enzyme)?

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Aids and Other Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Environmental Microbiology

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