Question 1

Since the rate of form movement in eukaryotes is much slower than that of bacteria, chromosomes require more than an hour to replicate.

Accepted Answer

A) True 
 B)False

Question 2

Why is only one copy of DNA made per cell division cycle in eukaryotes despite the presence of multiple origins of replication?

Accepted Answer

A)  There is only one origin of replication per chromosome. 
B)  The rate of elongation proceeds at a rate that allows only one copy to be made by the time the cell divides. 
C)  The activity of an S-phase protein kinase controls the formation of the pre-replication complex. 
D)  Primase is activated by phosphorylation only at the onset of S-phase by S-phase protein kinase. 
A)  There is only one origin of replication per chromosome. 
B)  The rate of elongation proceeds at a rate that allows only one copy to be made by the time the cell divides. 
C)  The activity of an S-phase protein kinase controls the formation of the pre-replication complex. 
D)  Primase is activated by phosphorylation only at the onset of S-phase by S-phase protein kinase.

Question 3

DNA polymerase III is the largest DNA polymerase in E. coli.

Accepted Answer

A) True 
 B)False

Question 4

Polymerization of the lagging strand is catalyzed by .

Accepted Answer

A)  a second core complex on the same polymerase holoenzyme that polymerizes the leading strand 
B)  a completely separate replisome from that which polymerizes the leading strand 
C)  DNA polymerase I holoenzyme 
D)  the same polymerase holoenzyme that polymerized the leading strand, but with opposite directionality 
A)  a second core complex on the same polymerase holoenzyme that polymerizes the leading strand 
B)  a completely separate replisome from that which polymerizes the leading strand 
C)  DNA polymerase I holoenzyme 
D)  the same polymerase holoenzyme that polymerized the leading strand, but with opposite directionality

Question 5

Prokaryotic DNA replication occurs in two steps. First, ATP provides a phosphate to the growing DNA chain. This is followed by addition of a nucleoside.

Accepted Answer

A) True 
 B)False

Question 6

A short primer is added to the DNA polymerase reaction mix for Sanger DNA sequencing that has a 5ʹ free hydroxyl to which new nucleotides can be added.

Accepted Answer

A) True 
 B)False

Question 7

Functions of DNA polymerases and accessory proteins in eukaryotic DNA replication include

Accepted Answer

A)  PCNA protein, a sliding clamp unit for DNA. 
B)  β- DNA polymerase, for repair of damaged DNA. 
C)  RPC protein, and equivalent of SSB proteins in bacteria. 
D)  RPC protein, used for mitochondrial DNA synthesis. 
E)  All of the above 
A)  PCNA protein, a sliding clamp unit for DNA. 
B)  β- DNA polymerase, for repair of damaged DNA. 
C)  RPC protein, and equivalent of SSB proteins in bacteria. 
D)  RPC protein, used for mitochondrial DNA synthesis. 
E)  All of the above

Question 8

The chromosome of the fruit fly D. melanogaster is about twice as large as the E. coli
chromosome.

Accepted Answer

A) True 
 B)False

Question 9

All recombination events involve homologous recombination between closely related DNA sequences.

Accepted Answer

A) True 
 B)False

Question 10

Although histones and DNA are made in the same parts of the eukaryotic cell, the packaging of them in the nucleosome slows down replication so that it is slower than that of prokaryotes.

Accepted Answer

A) True 
 B)False

Question 11

Which proteins are responsible for the unwinding of the double-stranded DNA during replication?

Accepted Answer

A)  ligases 
B)  helicases 
C)  topoisomerases 
D)  primases 
E)  lyases 
A)  ligases 
B)  helicases 
C)  topoisomerases 
D)  primases 
E)  lyases

Question 12

Nick translation is a process for

Accepted Answer

A)  filling in and sealing the gap in DNA after removal of the RNA primer. 
B)  synthesis of the protein for primase from mRNA. 
C)  removal of the RNA primer by DNA polymerase I. 
D)  removal of Okazaki fragments. 
A)  filling in and sealing the gap in DNA after removal of the RNA primer. 
B)  synthesis of the protein for primase from mRNA. 
C)  removal of the RNA primer by DNA polymerase I. 
D)  removal of Okazaki fragments.

Question 13

Which DNA polymerase is most responsible for chain elongation in E. coli?

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase II 
C)  DNA polymerase III 
D)  DNA polymerase IV 
A)  DNA polymerase I 
B)  DNA polymerase II 
C)  DNA polymerase III 
D)  DNA polymerase IV

Question 14

There are more replication forks during replication of eukaryotic DNA than prokaryotic DNA.

Accepted Answer

A) True 
 B)False

Question 15

Homologous recombination is the exchange of DNA between closely related DNA sequences.

Accepted Answer

A) True 
 B)False

Question 16

DNA that has been liberally labeled with radioactive 14C is used as the template for replication. Replication is carried out in a medium containing only unlabeled nucleotides. After two rounds of replication, what percent of DNA molecules are still labeled?

Accepted Answer

A)  25% 
B)  50% 
C)  75% 
D)  100% 
A)  25% 
B)  50% 
C)  75% 
D)  100%

Question 17

All DNA ligase enzymes use ATP as a cosubstrate.

Accepted Answer

A) True 
 B)False

Question 18

DNA polymerase I possesses 5ʹ → 3ʹ exonuclease activity.

Accepted Answer

A) True 
 B)False

Question 19

The e subunit of DNA polymerase III is responsible for its activity.

Accepted Answer

A)  5ʹ → 3ʹ polymerase 
B)  sliding clamp 
C)  3ʹ → 5ʹ exonuclease proof reading) 
D)  ribosome assembly 
A)  5ʹ → 3ʹ polymerase 
B)  sliding clamp 
C)  3ʹ → 5ʹ exonuclease proof reading) 
D)  ribosome assembly

Question 20

On average about twenty errors are made during each replication of the human genome.

Accepted Answer

A) True 
 B)False

Exam 20: Dna Replication, Repair, and Recombination

Since the rate of form movement in eukaryotes is much slower than that of bacteria, chromosomes require more than an hour to replicate.

Why is only one copy of DNA made per cell division cycle in eukaryotes despite the presence of multiple origins of replication?

DNA polymerase III is the largest DNA polymerase in E. coli.

Polymerization of the lagging strand is catalyzed by .

Prokaryotic DNA replication occurs in two steps. First, ATP provides a phosphate to the growing DNA chain. This is followed by addition of a nucleoside.

A short primer is added to the DNA polymerase reaction mix for Sanger DNA sequencing that has a 5ʹ free hydroxyl to which new nucleotides can be added.

Functions of DNA polymerases and accessory proteins in eukaryotic DNA replication include

The chromosome of the fruit fly D. melanogaster is about twice as large as the E. coli chromosome.

All recombination events involve homologous recombination between closely related DNA sequences.

Although histones and DNA are made in the same parts of the eukaryotic cell, the packaging of them in the nucleosome slows down replication so that it is slower than that of prokaryotes.

Which proteins are responsible for the unwinding of the double-stranded DNA during replication?

Nick translation is a process for

Which DNA polymerase is most responsible for chain elongation in E. coli?

There are more replication forks during replication of eukaryotic DNA than prokaryotic DNA.

Homologous recombination is the exchange of DNA between closely related DNA sequences.

DNA that has been liberally labeled with radioactive 14C is used as the template for replication. Replication is carried out in a medium containing only unlabeled nucleotides. After two rounds of replication, what percent of DNA molecules are still labeled?

All DNA ligase enzymes use ATP as a cosubstrate.

DNA polymerase I possesses 5ʹ → 3ʹ exonuclease activity.

The e subunit of DNA polymerase III is responsible for its activity.

On average about twenty errors are made during each replication of the human genome.

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Transcription and Rna Processing

Protein Synthesis

Recombinant Dna Technology

Filters

DNA that has been liberally labeled with radioactive ¹⁴C is used as the template for replication. Replication is carried out in a medium containing only unlabeled nucleotides. After two rounds of replication, what percent of DNA molecules are still labeled?