Question 1

In the Holliday model describing recombination

Accepted Answer

A)  homologous sequences line up. 
B)  strands of DNA are nicked. 
C)  DNA strands associate with the homologous strand ʺstrand invasionʺ). 
D)  strands rotate then are cleaved at the crossover point. 
E)  All of the above 
A)  homologous sequences line up. 
B)  strands of DNA are nicked. 
C)  DNA strands associate with the homologous strand ʺstrand invasionʺ). 
D)  strands rotate then are cleaved at the crossover point. 
E)  All of the above

Question 2

Proteins bind to both origin sites and termination sites on DNA during DNA replication.

Accepted Answer

A) True 
 B)False

Question 3

DNA repair cannot be done without breaking the phosphodiester backbone of DNA.

Accepted Answer

A) True 
 B)False

Question 4

In packaging the eukaryotic DNA into nucleosomes, it is thought that the requirement for and their synthesis is a cause of the slower rate of movement of the replication fork.

Accepted Answer

A)  Okazaki fragments 
B)  larger DNA molecules 
C)  increase number of accessory proteins 
D)  histone proteins 
E)  lagging strands 
A)  Okazaki fragments 
B)  larger DNA molecules 
C)  increase number of accessory proteins 
D)  histone proteins 
E)  lagging strands

Question 5

The incorporation of new nucleotides into a growing strand of DNA occurs during .

Accepted Answer

A)  the elongation stage 
B)  nucleation 
C)  initiation 
D)  assembly of the protein machine 
A)  the elongation stage 
B)  nucleation 
C)  initiation 
D)  assembly of the protein machine

Question 6

During recombination in E. coli, recognizes) regions of sequence similarity and promotes) strand invasion, while binds) to a Holliday junction promoting strand migration.

Accepted Answer

A)  RuvA and RuvB; RecBCD 
B)  RecBCD; RecA 
C)  RecA; RecBCD 
D)  RecA; RuvA and RuvB 
A)  RuvA and RuvB; RecBCD 
B)  RecBCD; RecA 
C)  RecA; RecBCD 
D)  RecA; RuvA and RuvB

Question 7

Okazaki fragments are .

Accepted Answer

A)  the smallest subunits of DNA polymerase III 
B)  short stretches of DNA formed on the lagging strand 
C)  short RNA primers needed for initiation of polymerization 
D)  fragments of DNA polymerase I that lack 5ʹ → 3ʹ exonuclease activity 
A)  the smallest subunits of DNA polymerase III 
B)  short stretches of DNA formed on the lagging strand 
C)  short RNA primers needed for initiation of polymerization 
D)  fragments of DNA polymerase I that lack 5ʹ → 3ʹ exonuclease activity

Question 8

The reaction catalyzed by DNA photolyase is an example of what type of repair?

Accepted Answer

A)  recombination 
B)  excision 
C)  mismatch 
D)  direct 
A)  recombination 
B)  excision 
C)  mismatch 
D)  direct

Question 9

At the normal rate of replication, about how many minutes does it take for the entire E. coli chromosome 4.6 × 103 kbp) to be duplicated?

Accepted Answer

A)  5 
B)  20 
C)  40 
D)  80 
A)  5 
B)  20 
C)  40 
D)  80

Question 10

The sequence of genes and proteins used in the initiation and termination of DNA replication is

Accepted Answer

A)  Tus → ter → origin → DnaA → dnaA 
B)  dnaA → DnaA → origin → Tus → ter 
C)  Origin → DnaA → dnaA → ter → Tus 
D)  DnaA → ter → Tus → origin → ter → dnaA 
A)  Tus → ter → origin → DnaA → dnaA 
B)  dnaA → DnaA → origin → Tus → ter 
C)  Origin → DnaA → dnaA → ter → Tus 
D)  DnaA → ter → Tus → origin → ter → dnaA

Question 11

The Klenow fragment can be used for DNA sequencing if proteins such as SSB protein are added.

Accepted Answer

A) True 
 B)False

Question 12

Enzymes that remain bound to their nascent chains through many polymerization steps are said to be .

Accepted Answer

A)  constitutive 
B)  distributive 
C)  conjugated 
D)  processive 
A)  constitutive 
B)  distributive 
C)  conjugated 
D)  processive

Question 13

Why does E. coli need both DNA polymerse III and DNA polymerase I?

Accepted Answer

A)  DNA polymerase III lacks the 5ʹ → 3ʹ exonuclease activity needed to remove RNA primers. 
B)  Each polymerase is specific for only one strand of DNA. DNA polymerase III acts only on the leading strand, and DNA polymerase I acts only on the lagging strand. 
C)  DNA polymerase I is needed to join the Okazaki fragments. 
D)  The DNA replication is bidirectional; one polymerase is used for each direction. 
E)  Only DNA polymerase I has proofreading ability. 
A)  DNA polymerase III lacks the 5ʹ → 3ʹ exonuclease activity needed to remove RNA primers. 
B)  Each polymerase is specific for only one strand of DNA. DNA polymerase III acts only on the leading strand, and DNA polymerase I acts only on the lagging strand. 
C)  DNA polymerase I is needed to join the Okazaki fragments. 
D)  The DNA replication is bidirectional; one polymerase is used for each direction. 
E)  Only DNA polymerase I has proofreading ability.

Question 14

During the elongation stage

Accepted Answer

A)  replication proteins assemble at the DNA replication site. 
B)  DNA is replicated semiconservatively. 
C)  the protein machine disassembles. 
D)  replication ends. 
A)  replication proteins assemble at the DNA replication site. 
B)  DNA is replicated semiconservatively. 
C)  the protein machine disassembles. 
D)  replication ends.

Question 15

The best enzyme to use in the Sanger method for DNA sequence determination is

Accepted Answer

A)  DNA polymerase I . 
B)  Klenow fragment of DNA polymerase I. 
C)  DNA polymerase from a high temperature bacterium. 
D)  SSB protein. 
A)  DNA polymerase I . 
B)  Klenow fragment of DNA polymerase I. 
C)  DNA polymerase from a high temperature bacterium. 
D)  SSB protein.

Question 16

The sequence of proteins used in bacterial recombination is

Accepted Answer

A)  RuvC → RuvB → RecA → RecBCD 
B)  RecBCD → RecA → RuvAB → RuvC 
C)  RecA → RecBCD → RuvAB → RuvC 
D)  RuvAB → RuvC → RecBCD → RecA 
A)  RuvC → RuvB → RecA → RecBCD 
B)  RecBCD → RecA → RuvAB → RuvC 
C)  RecA → RecBCD → RuvAB → RuvC 
D)  RuvAB → RuvC → RecBCD → RecA

In the Holliday model describing recombination

Proteins bind to both origin sites and termination sites on DNA during DNA replication.

DNA repair cannot be done without breaking the phosphodiester backbone of DNA.

In packaging the eukaryotic DNA into nucleosomes, it is thought that the requirement for and their synthesis is a cause of the slower rate of movement of the replication fork.

The incorporation of new nucleotides into a growing strand of DNA occurs during .

During recombination in E. coli, recognizes) regions of sequence similarity and promotes) strand invasion, while binds) to a Holliday junction promoting strand migration.

Okazaki fragments are .

The reaction catalyzed by DNA photolyase is an example of what type of repair?

At the normal rate of replication, about how many minutes does it take for the entire E. coli chromosome 4.6 × 10³kbp) to be duplicated?

The sequence of genes and proteins used in the initiation and termination of DNA replication is

The Klenow fragment can be used for DNA sequencing if proteins such as SSB protein are added.

Enzymes that remain bound to their nascent chains through many polymerization steps are said to be .

Why does E. coli need both DNA polymerse III and DNA polymerase I?

During the elongation stage

The best enzyme to use in the Sanger method for DNA sequence determination is

The sequence of proteins used in bacterial recombination is

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Transcription and Rna Processing

Protein Synthesis

Recombinant Dna Technology

Filters

Exam 20: Dna Replication, Repair, and Recombination

In the Holliday model describing recombination

Proteins bind to both origin sites and termination sites on DNA during DNA replication.

DNA repair cannot be done without breaking the phosphodiester backbone of DNA.

In packaging the eukaryotic DNA into nucleosomes, it is thought that the requirement for and their synthesis is a cause of the slower rate of movement of the replication fork.

The incorporation of new nucleotides into a growing strand of DNA occurs during .

During recombination in E. coli, recognizes) regions of sequence similarity and promotes) strand invasion, while binds) to a Holliday junction promoting strand migration.

Okazaki fragments are .

The reaction catalyzed by DNA photolyase is an example of what type of repair?

At the normal rate of replication, about how many minutes does it take for the entire E. coli chromosome 4.6 × 103 kbp) to be duplicated?

The sequence of genes and proteins used in the initiation and termination of DNA replication is

The Klenow fragment can be used for DNA sequencing if proteins such as SSB protein are added.

Enzymes that remain bound to their nascent chains through many polymerization steps are said to be .

Why does E. coli need both DNA polymerse III and DNA polymerase I?

During the elongation stage

The best enzyme to use in the Sanger method for DNA sequence determination is

The sequence of proteins used in bacterial recombination is

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Transcription and Rna Processing

Protein Synthesis

Recombinant Dna Technology

Filters

At the normal rate of replication, about how many minutes does it take for the entire E. coli chromosome 4.6 × 10³kbp) to be duplicated?