Question 1

Which of the following is true of all biofilms?

Accepted Answer

A)  They only contain a single species. 
B)  The EPS are composed of proteins. 
C)  Cells in the biofilm are dormant until the bacteria leave the biofilm. 
D)  The EPS is secreted by the bacteria. 
E)  It is a continuous monolayer surface deposit. 
A)  They only contain a single species. 
B)  The EPS are composed of proteins. 
C)  Cells in the biofilm are dormant until the bacteria leave the biofilm. 
D)  The EPS is secreted by the bacteria. 
E)  It is a continuous monolayer surface deposit.

Question 2

Which of the following occurs when a sessile biofilm (or a part of it) begins to starve or experiences oxygen depletion?

Accepted Answer

A)  attachment 
B)  quorum sensing 
C)  EPS production 
D)  dispersal 
E)  biofilm maturation 
A)  attachment 
B)  quorum sensing 
C)  EPS production 
D)  dispersal 
E)  biofilm maturation

Question 3

MacConkey medium contains bile salts, crystal violet, lactose, and the pH indicator neutral red. Some organisms can ferment lactose, producing acids as a by-product. Is this medium likely to be selective and/or differential? Explain.

Accepted Answer

Since MacConkey contains bile salts and

Question 4

You are measuring growth in a bacterial culture. The number of viable cells remains constant, and when you check the medium, you find that the carbon source has been used up. In which phase of growth is the culture?

Accepted Answer

A)  lag 
B)  early log 
C)  late log 
D)  stationary 
E)  death 
A)  lag 
B)  early log 
C)  late log 
D)  stationary 
E)  death

Question 5

Which of the following is NOT true about the substrate-binding protein (SBP) of the ABC transport system?

Accepted Answer

A)  It floats in the periplasm of Gram-negative cells. 
B)  It is tethered to the cell surface in Gram-positive cells. 
C)  It binds to the periplasmic face of the channel and releases the solute. 
D)  It snags the appropriate solute. 
E)  It is required for both the uptake and efflux systems. 
A)  It floats in the periplasm of Gram-negative cells. 
B)  It is tethered to the cell surface in Gram-positive cells. 
C)  It binds to the periplasmic face of the channel and releases the solute. 
D)  It snags the appropriate solute. 
E)  It is required for both the uptake and efflux systems.

Question 6

In order to grow unculturable environmental microbes, Kim Lewis and colleagues derived the iChip. What is the purpose of this device?

Accepted Answer

A)  Antibiotics from the environment could pass into the chamber. 
B)  Gram-positive organisms could not pass the semipermeable membrane. 
C)  The semipermeable membranes let environmental organisms into the agar. 
D)  Organisms from the environment grew in the chamber. 
E)  Nutrients and growth factors made by organisms in the soil diffused into the chamber. 
A)  Antibiotics from the environment could pass into the chamber. 
B)  Gram-positive organisms could not pass the semipermeable membrane. 
C)  The semipermeable membranes let environmental organisms into the agar. 
D)  Organisms from the environment grew in the chamber. 
E)  Nutrients and growth factors made by organisms in the soil diffused into the chamber.

Question 7

What type of medium and conditions would you need to have in order to select for photoautotrophs?

Accepted Answer

Phototrophs can generate energy from abs

Question 8

Quorum sensing begins during which growth stage?

Accepted Answer

A)  lag 
B)  early log 
C)  late log 
D)  stationary 
E)  death 
A)  lag 
B)  early log 
C)  late log 
D)  stationary 
E)  death

Question 9

Using the figure below, explain the function of stages 2 and 3 in biofilm development and how they affect biofilm growth, function, and treatment.

Accepted Answer

The establishment of microcolonies trigg

Question 10

Which term refers to the condition in which a culture growth covers the entire agar surface?

Accepted Answer

A)  dilution streaking 
B)  spread plate 
C)  confluent 
D)  microcolony 
E)  colony 
A)  dilution streaking 
B)  spread plate 
C)  confluent 
D)  microcolony 
E)  colony

Which of the following is true of all biofilms?

Which of the following occurs when a sessile biofilm (or a part of it) begins to starve or experiences oxygen depletion?

MacConkey medium contains bile salts, crystal violet, lactose, and the pH indicator neutral red. Some organisms can ferment lactose, producing acids as a by-product. Is this medium likely to be selective and/or differential? Explain.

You are measuring growth in a bacterial culture. The number of viable cells remains constant, and when you check the medium, you find that the carbon source has been used up. In which phase of growth is the culture?

Which of the following is NOT true about the substrate-binding protein (SBP) of the ABC transport system?

In order to grow unculturable environmental microbes, Kim Lewis and colleagues derived the iChip. What is the purpose of this device?

What type of medium and conditions would you need to have in order to select for photoautotrophs?

Quorum sensing begins during which growth stage?

Using the figure below, explain the function of stages 2 and 3 in biofilm development and how they affect biofilm growth, function, and treatment.

Which term refers to the condition in which a culture growth covers the entire agar surface?

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Environmental Influences and Control of Microbial Growth

Viruses

Genomes and Chromosomes

Transcription, Translation, and Bioinformatics

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Biotechniques and Synthetic Biology

Energetics and Catabolism

Electron Flow in Organotrophy, Lithotrophy, and Phototrophy

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes in Global Elemental Cycles

Human Microbiota and Innate Immunity

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Therapy

Clinical Microbiology and Epidemiology

Filters

Exam 4: Bacterial Culture, Growth, and Development

Which of the following is true of all biofilms?

Which of the following occurs when a sessile biofilm (or a part of it) begins to starve or experiences oxygen depletion?

MacConkey medium contains bile salts, crystal violet, lactose, and the pH indicator neutral red. Some organisms can ferment lactose, producing acids as a by-product. Is this medium likely to be selective and/or differential? Explain.

You are measuring growth in a bacterial culture. The number of viable cells remains constant, and when you check the medium, you find that the carbon source has been used up. In which phase of growth is the culture?

Which of the following is NOT true about the substrate-binding protein (SBP) of the ABC transport system?

In order to grow unculturable environmental microbes, Kim Lewis and colleagues derived the iChip. What is the purpose of this device?

What type of medium and conditions would you need to have in order to select for photoautotrophs?

Quorum sensing begins during which growth stage?

Using the figure below, explain the function of stages 2 and 3 in biofilm development and how they affect biofilm growth, function, and treatment.

Which term refers to the condition in which a culture growth covers the entire agar surface?

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Environmental Influences and Control of Microbial Growth

Viruses

Genomes and Chromosomes

Transcription, Translation, and Bioinformatics

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Biotechniques and Synthetic Biology

Energetics and Catabolism

Electron Flow in Organotrophy, Lithotrophy, and Phototrophy

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes in Global Elemental Cycles

Human Microbiota and Innate Immunity

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Therapy

Clinical Microbiology and Epidemiology

Filters