Exam 12: Biotechniques and Synthetic Biology

Annotation of sequences of transposon insertion sites in the chromosome of Escherichia coli O157:H7 cells that had lost their acid-resistance properties revealed four genes: two encoding isoforms of glutamate decarboxylase (GadA and GadB) and one encoding a glutamate/gamma-aminobutyric acid (GABA) antiporter. What does the fourth gene encode?

Advantages in real-time PCR over regular, end-time PCR include all of the following EXCEPT

Which of the following experimental procedures is NOT used in ChIP-seq?

The applications of the yeast two-hybrid method include all of the following EXCEPT

In a yeast two-hybrid analysis of several strains containing different prey/bait combinations, the screening of interacting proteins is done by growing cells on a medium containing X-Gal. White and blue colonies are observed after incubation. Which of the following statements is NOT true regarding blue/white selection in the yeast two-hybrid method?

The phenomenon that allows a quencher dye to reduce the fluorescence of the reporter dye in real-time PCR techniques is called

Both southern and northern blots involve the use of agarose gels. How do the types of agarose gels differ and why?

Genes coding for insecticidal peptides have been engineered into the genomes of some crops. Which of these peptides are naturally produced by the bacterium?

Acid-resistant Escherichia coli cells counteract H⁺ in their cytoplasm through the combined action of two proteins: a decarboxylase enzyme and

What are translational fusions used for? How are they constructed?

Describe the use of a primary and secondary antibody in a western blot.

A protein of interest can be specifically detected on western blots; this technique requires all of the following EXCEPT