Question 1

The endospore stain reveals internal structures within cells of the genera Bacillus and Clostridium.

Accepted Answer

A) True 
 B)False  True

Question 2

All of the following are common to both the Gram stain and the acid-fast stain EXCEPT

Accepted Answer

A)  primary stain. 
B)  counterstain. 
C)  a decolorizing agent. 
D)  a chemical mordant. 
E)  a decolorizing agent and a counterstain. 
A)  primary stain. 
B)  counterstain. 
C)  a decolorizing agent. 
D)  a chemical mordant. 
E)  a decolorizing agent and a counterstain. D

Question 3

A (decolorizer/mordant/fixer)is a substance that binds to a dye and makes it less soluble.

Accepted Answer

mordant

Question 4

Carbolfuchsin is the ________ in the acid-fast stain.

Accepted Answer

A)  primary stain 
B)  mordant 
C)  decolorizer 
D)  counterstain 
E)  fixing reagent 
A)  primary stain 
B)  mordant 
C)  decolorizer 
D)  counterstain 
E)  fixing reagent

Question 5

PCR is a method for identifying microbes based on their (antigens/genes/morphology).

Accepted Answer

The answer of PCR is a method for identifying microbes...

Question 6

You are shown a micrograph from a light microscope in which the specimens appear bright compared to the background.The micrograph is probably from a(n)________ microscope.

Accepted Answer

A)  dark-field 
B)  phase-contrast 
C)  Nomarski 
D)  bright-field 
E)  atomic force 
A)  dark-field 
B)  phase-contrast 
C)  Nomarski 
D)  bright-field 
E)  atomic force

Question 7

The rules of naming organisms are called

Accepted Answer

A)  taxonomy. 
B)  nomenclature. 
C)  classification. 
D)  binomials. 
E)  identification. 
A)  taxonomy. 
B)  nomenclature. 
C)  classification. 
D)  binomials. 
E)  identification.

Question 8

In a transmission electron microscope,the "lenses" are

Accepted Answer

A)  made of glass. 
B)  thin films of metal. 
C)  lasers. 
D)  magnets. 
E)  vacuums. 
A)  made of glass. 
B)  thin films of metal. 
C)  lasers. 
D)  magnets. 
E)  vacuums.

Question 9

Viruses are generally measured in

Accepted Answer

A)  nanometers. 
B)  millimeters. 
C)  micrometers. 
D)  centimeters. 
E)  decimeters. 
A)  nanometers. 
B)  millimeters. 
C)  micrometers. 
D)  centimeters. 
E)  decimeters.

Question 10

A measurement of a microbe is reported as 1 × 10-6 m,also known as

Accepted Answer

A)  centimeters (cm). 
B)  millimeters (mm). 
C)  micrometers (μm). 
D)  nanometers (nm). 
E)  yards. 
A)  centimeters (cm). 
B)  millimeters (mm). 
C)  micrometers (μm). 
D)  nanometers (nm). 
E)  yards.

Question 11

All types of radiation are used for microscopy.

Accepted Answer

A) True 
 B)False

Question 12

A primary purpose for the use of stains in microscopy is to increase the (magnification/brightness/contrast)of a specimen.

Accepted Answer

The answer of A primary purpose for the use of...

Question 13

A virologist wants to observe the surface features of virus particles she is studying.Which of the following microscopes would NOT be useful for her observations?

Accepted Answer

A)  differential interference contrast 
B)  scanning tunneling 
C)  scanning electron 
D)  transmission electron 
E)  atomic force 
A)  differential interference contrast 
B)  scanning tunneling 
C)  scanning electron 
D)  transmission electron 
E)  atomic force

Question 14

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

Accepted Answer

Light rays can be manipulated to increas

Question 15

Safranin dye is used as the counterstain in ________ stain(s).

Accepted Answer

A)  the Gram 
B)  the endospore 
C)  the acid-fast 
D)  the flagellar 
E)  both the Gram and the endospore 
A)  the Gram 
B)  the endospore 
C)  the acid-fast 
D)  the flagellar 
E)  both the Gram and the endospore

Question 16

The part of the microscope indicated by the arrow in Figure 4.1 is the (ocular/objective/condenser)lens.

Accepted Answer

The answer of   
The part of the microscope...

Question 17

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron)microscopy.

Accepted Answer

The answer of Coating a specimen with a heavy metal...

Question 18

In the Schaffer-Fulton endospore stain,heat is a (fixation/mordant/staining)step.

Accepted Answer

The answer of In the Schaffer-Fulton endospore stain,heat is a...

Question 19

Phage typing is useful for identifying bacteria because of the specificity of (antibodies/bacteriophages/PCR)for unique bacterial structures.

Accepted Answer

The answer of Phage typing is useful for identifying bacteria...

Question 20

All of the following are types of light microscopes EXCEPT

Accepted Answer

A)  fluorescent. 
B)  confocal. 
C)  phase-contrast. 
D)  scanning tunneling. 
E)  bright-field. 
A)  fluorescent. 
B)  confocal. 
C)  phase-contrast. 
D)  scanning tunneling. 
E)  bright-field.

Exam 4: Microscopy, Staining, and Classification

The endospore stain reveals internal structures within cells of the genera Bacillus and Clostridium.

All of the following are common to both the Gram stain and the acid-fast stain EXCEPT

A (decolorizer/mordant/fixer)is a substance that binds to a dye and makes it less soluble.

Carbolfuchsin is the ________ in the acid-fast stain.

PCR is a method for identifying microbes based on their (antigens/genes/morphology).

You are shown a micrograph from a light microscope in which the specimens appear bright compared to the background.The micrograph is probably from a(n)________ microscope.

The rules of naming organisms are called

In a transmission electron microscope,the "lenses" are

Viruses are generally measured in

A measurement of a microbe is reported as 1 × 10-6 m,also known as

All types of radiation are used for microscopy.

A primary purpose for the use of stains in microscopy is to increase the (magnification/brightness/contrast)of a specimen.

A virologist wants to observe the surface features of virus particles she is studying.Which of the following microscopes would NOT be useful for her observations?

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

Safranin dye is used as the counterstain in ________ stain(s).

The part of the microscope indicated by the arrow in Figure 4.1 is the (ocular/objective/condenser)lens.

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron)microscopy.

In the Schaffer-Fulton endospore stain,heat is a (fixation/mordant/staining)step.

Phage typing is useful for identifying bacteria because of the specificity of (antibodies/bacteriophages/PCR)for unique bacterial structures.

All of the following are types of light microscopes EXCEPT

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant DNA Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic DNA Viruses

Pathogenic RNA Viruses

Applied and Environmental Microbiology

Filters