Question 1

Which of the following are magnifying lenses?

Accepted Answer

A)  objectives 
B)  oculars 
C)  condensers 
D)  prisms 
E)  both objectives and the oculars 
A)  objectives 
B)  oculars 
C)  condensers 
D)  prisms 
E)  both objectives and the oculars

Question 2

Gram staining of bacteria provides all the physical characterization necessary to identify bacterial species.

Accepted Answer

A) True 
 B)False

Question 3

The microscope preferred for viewing living specimens is the ________ microscope.

Accepted Answer

A)  bright-field 
B)  phase-contrast 
C)  scanning electron 
D)  scanning tunneling 
E)  transmission electron 
A)  bright-field 
B)  phase-contrast 
C)  scanning electron 
D)  scanning tunneling 
E)  transmission electron

Question 4

The placement of an organism into a domain is made on the basis of

Accepted Answer

A)  G + C content. 
B)  cell ultrastructure. 
C)  ribosomal RNA analysis. 
D)  serological tests. 
E)  Gram-stain reactions. 
A)  G + C content. 
B)  cell ultrastructure. 
C)  ribosomal RNA analysis. 
D)  serological tests. 
E)  Gram-stain reactions.

Question 5

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Accepted Answer

A)  transfer RNA 
B)  membrane lipids 
C)  ribosomal RNA 
D)  DNA 
E)  proteins 
A)  transfer RNA 
B)  membrane lipids 
C)  ribosomal RNA 
D)  DNA 
E)  proteins

Question 6

Acidic dyes

Accepted Answer

A)  work best in low pH environments. 
B)  are negatively charged. 
C)  are used for staining negatively charged molecular structures. 
D)  are lipid soluble. 
E)  are negatively charged and work best at low pH. 
A)  work best in low pH environments. 
B)  are negatively charged. 
C)  are used for staining negatively charged molecular structures. 
D)  are lipid soluble. 
E)  are negatively charged and work best at low pH.

Question 7

Which of the following is NOT associated with an electron microscope?

Accepted Answer

A)  an electron beam 
B)  magnetic fields 
C)  a prism 
D)  a fluorescent screen 
E)  a vacuum 
A)  an electron beam 
B)  magnetic fields 
C)  a prism 
D)  a fluorescent screen 
E)  a vacuum

Question 8

Why are modern light microscopes better than the ones Leeuwenhoek used?

Accepted Answer

A)  Modern microscopes have a fivefold better resolution. 
B)  Modern microscopes are compound instead of simple. 
C)  Modern microscopes have lenses with smaller numerical apertures. 
D)  Modern lenses are made of prisms. 
E)  Modern microscopes are compound and have fivefold better resolution. 
A)  Modern microscopes have a fivefold better resolution. 
B)  Modern microscopes are compound instead of simple. 
C)  Modern microscopes have lenses with smaller numerical apertures. 
D)  Modern lenses are made of prisms. 
E)  Modern microscopes are compound and have fivefold better resolution.

Question 9

Which of the following statements about transmission electron microscopy is CORRECT?

Accepted Answer

A)  Three-dimensional images are produced. 
B)  Lasers are used for visualization. 
C)  Up to 1,000,000X magnification may be achieved. 
D)  Living specimens may be used. 
E)  Stains can be applied to create a color image. 
A)  Three-dimensional images are produced. 
B)  Lasers are used for visualization. 
C)  Up to 1,000,000X magnification may be achieved. 
D)  Living specimens may be used. 
E)  Stains can be applied to create a color image.

Question 10

Compare and contrast the light microscope with the electron microscope.

Accepted Answer

Both the light microscope and the electr

Question 11

A resolution of 1 μm would be better than a resolution of 0.5 μm.

Accepted Answer

A) True 
 B)False

Question 12

In the Gram stain procedure,iodine serves as a

Accepted Answer

A)  counterstain. 
B)  decolorizing agent. 
C)  mordant. 
D)  primary stain. 
E)  fixative. 
A)  counterstain. 
B)  decolorizing agent. 
C)  mordant. 
D)  primary stain. 
E)  fixative.

Question 13

The resolution of a microscope lens is a function of the lens' (aperture/color/contrast).

Accepted Answer

The answer of The resolution of a microscope lens is...

Question 14

Light rays that pass through the edge of a curved lens will be refracted more than those that pass through the center.

Accepted Answer

A) True 
 B)False

Question 15

One-thousandth of a meter is a

Accepted Answer

A)  yard. 
B)  millimeter (mm). 
C)  micrometer (μm). 
D)  nanometer (nm). 
E)  centimeter (cm). 
A)  yard. 
B)  millimeter (mm). 
C)  micrometer (μm). 
D)  nanometer (nm). 
E)  centimeter (cm).

Question 16

Carl Woese and George Fox proposed the (phylum/domain/family),a taxon that contains multiple kingdoms.

Accepted Answer

The answer of Carl Woese and George Fox proposed the...

Question 17

A single basic dye is used in simple stains.

Accepted Answer

A) True 
 B)False

Question 18

The kingdoms included in the Linnaeus system of classification are

Accepted Answer

A)  Animalia and Prokaryotae. 
B)  Protista and Plantae. 
C)  Fungi and Protista. 
D)  Animalia and Plantae. 
E)  Prokaryotae and Protista. 
A)  Animalia and Prokaryotae. 
B)  Protista and Plantae. 
C)  Fungi and Protista. 
D)  Animalia and Plantae. 
E)  Prokaryotae and Protista.

Question 19

In Gram staining,ethanol-acetone is used as a

Accepted Answer

A)  decolorizing agent. 
B)  counterstain. 
C)  mordant. 
D)  drying agent. 
E)  primary stain. 
A)  decolorizing agent. 
B)  counterstain. 
C)  mordant. 
D)  drying agent. 
E)  primary stain.

Question 20

Acid-fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid.

Accepted Answer

A) True 
 B)False

Exam 4: Microscopy, Staining, and Classification

Which of the following are magnifying lenses?

Gram staining of bacteria provides all the physical characterization necessary to identify bacterial species.

The microscope preferred for viewing living specimens is the ________ microscope.

The placement of an organism into a domain is made on the basis of

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Acidic dyes

Which of the following is NOT associated with an electron microscope?

Why are modern light microscopes better than the ones Leeuwenhoek used?

Which of the following statements about transmission electron microscopy is CORRECT?

Compare and contrast the light microscope with the electron microscope.

A resolution of 1 μm would be better than a resolution of 0.5 μm.

In the Gram stain procedure,iodine serves as a

The resolution of a microscope lens is a function of the lens' (aperture/color/contrast).

Light rays that pass through the edge of a curved lens will be refracted more than those that pass through the center.

One-thousandth of a meter is a

Carl Woese and George Fox proposed the (phylum/domain/family),a taxon that contains multiple kingdoms.

A single basic dye is used in simple stains.

The kingdoms included in the Linnaeus system of classification are

In Gram staining,ethanol-acetone is used as a

Acid-fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant DNA Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic DNA Viruses

Pathogenic RNA Viruses

Applied and Environmental Microbiology

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