Question 1

Some of the strongest opposition to the application of recombinant DNA technology concerns

Accepted Answer

A)  DNA fingerprinting. 
B)  modification of food crops. 
C)  correcting gene defects in animals. 
D)  sequencing of the human genome. 
E)  pest control measures. 
A)  DNA fingerprinting. 
B)  modification of food crops. 
C)  correcting gene defects in animals. 
D)  sequencing of the human genome. 
E)  pest control measures. B

Question 2

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure,what would be the result?

Accepted Answer

A)  DNA replication would occur more slowly than normal. 
B)  DNA replication would not occur at all. 
C)  Many mistakes would occur. 
D)  DNA replication would occur twice as fast as normal. 
E)  DNA replication would stop after one cycle. 
A)  DNA replication would occur more slowly than normal. 
B)  DNA replication would not occur at all. 
C)  Many mistakes would occur. 
D)  DNA replication would occur twice as fast as normal. 
E)  DNA replication would stop after one cycle. E

Question 3

Restriction enzymes are useful only on synthetic DNAs.

Accepted Answer

A) True 
 B)False  False

Question 4

Beta-carotene,the biochemical precursor to vitamin A,can be added to rice by using (biotechnology/cloning/recombinant)DNA technology,thereby increasing its nutritional value.

Accepted Answer

The answer of Beta-carotene,the biochemical precursor to vitamin A,can be...

Question 5

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Accepted Answer

A)  Deinococcus radiodurans 
B)  Bacillus thuringiensis 
C)  Pseudomonas 
D)  Salmonella 
E)  Escherichia coli 
A)  Deinococcus radiodurans 
B)  Bacillus thuringiensis 
C)  Pseudomonas 
D)  Salmonella 
E)  Escherichia coli

Question 6

A technique using fluorescent-tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/PCR/BLOTTING).

Accepted Answer

The answer of A technique using fluorescent-tagged probes to detect...

Question 7

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Accepted Answer

A) True 
 B)False

Question 8

Gene therapy for human genetic diseases has not been successful yet.

Accepted Answer

A) True 
 B)False

Question 9

Which of the following is common to both Sanger sequencing and next-generation sequencing?

Accepted Answer

A)  radioactive labels 
B)  gel electrophoresis 
C)  tethered DNA fragments 
D)  chain terminating nucleotides 
E)  lasers 
A)  radioactive labels 
B)  gel electrophoresis 
C)  tethered DNA fragments 
D)  chain terminating nucleotides 
E)  lasers

Question 10

Which of the following items is NOT a part of the name of a restriction enzyme?

Accepted Answer

A)  the Gram reaction of the source bacterium 
B)  the specific epithet of the source bacterium 
C)  Roman numerals to indicate its order of discovery 
D)  the strain of the source bacterium 
E)  the genus of the source bacterium 
A)  the Gram reaction of the source bacterium 
B)  the specific epithet of the source bacterium 
C)  Roman numerals to indicate its order of discovery 
D)  the strain of the source bacterium 
E)  the genus of the source bacterium

Question 11

________ are used in the detection of DNA by FISH.

Accepted Answer

A)  Restriction fragments 
B)  Reverse transcriptase and DNA polymerase 
C)  Synthetic DNAs and fluorescent tags 
D)  Compressed air and gold beads 
E)  DNA polymerase and DNA ligase 
A)  Restriction fragments 
B)  Reverse transcriptase and DNA polymerase 
C)  Synthetic DNAs and fluorescent tags 
D)  Compressed air and gold beads 
E)  DNA polymerase and DNA ligase

Question 12

The templates for next-generation sequencing are

Accepted Answer

A)  double-strand restriction fragments. 
B)  double-strand DNA on silicon chips. 
C)  double-strand PCR products. 
D)  single-strand DNA on silicon chips. 
E)  single-strand DNA on slides. 
A)  double-strand restriction fragments. 
B)  double-strand DNA on silicon chips. 
C)  double-strand PCR products. 
D)  single-strand DNA on silicon chips. 
E)  single-strand DNA on slides.

Question 13

Which of the following devices is used for PCR?

Accepted Answer

A)  an electrophoresis chamber 
B)  a gene gun 
C)  a DNA sequencer 
D)  a thermocycler 
E)  a nucleic acid synthesis machine 
A)  an electrophoresis chamber 
B)  a gene gun 
C)  a DNA sequencer 
D)  a thermocycler 
E)  a nucleic acid synthesis machine

Question 14

Which of the following microbes produces a protein that kills a variety of insect pests?

Accepted Answer

A)  Pseudomonas 
B)  Haemophilus influenzae 
C)  Bacillus thuringiensis 
D)  Thermus aquaticus 
E)  Plasmodium falciparum 
A)  Pseudomonas 
B)  Haemophilus influenzae 
C)  Bacillus thuringiensis 
D)  Thermus aquaticus 
E)  Plasmodium falciparum

Question 15

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

Accepted Answer

A)  microarray 
B)  cloning 
C)  PCR 
D)  genome mapping 
E)  microarray or PCR 
A)  microarray 
B)  cloning 
C)  PCR 
D)  genome mapping 
E)  microarray or PCR

Question 16

The natural role of restriction enzymes in bacteria is to

Accepted Answer

A)  make conjugation more efficient. 
B)  allow transposons to move to another place in the chromosome. 
C)  protect the cell from invading phages. 
D)  allow cells to accept foreign DNA. 
E)  provide the cell with new phenotypes, such as antibiotic resistance. 
A)  make conjugation more efficient. 
B)  allow transposons to move to another place in the chromosome. 
C)  protect the cell from invading phages. 
D)  allow cells to accept foreign DNA. 
E)  provide the cell with new phenotypes, such as antibiotic resistance.

Question 17

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Question 18

Which of the following is essential in PCR?

Accepted Answer

A)  DNA polymerase 
B)  antisense RNAs 
C)  reverse transcriptase 
D)  DNA primers 
E)  both DNA primers and DNA polymerase 
A)  DNA polymerase 
B)  antisense RNAs 
C)  reverse transcriptase 
D)  DNA primers 
E)  both DNA primers and DNA polymerase

Question 19

Pest resistant cotton plants that produce Bt were produced by selective breeding.

Accepted Answer

A) True 
 B)False

Question 20

Explain what a transgenic organism is,and give two examples.

Accepted Answer

A transgenic organism is one that contai

Exam 8: Recombinant DNA Technology

Some of the strongest opposition to the application of recombinant DNA technology concerns

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure,what would be the result?

Restriction enzymes are useful only on synthetic DNAs.

Beta-carotene,the biochemical precursor to vitamin A,can be added to rice by using (biotechnology/cloning/recombinant)DNA technology,thereby increasing its nutritional value.

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

A technique using fluorescent-tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/PCR/BLOTTING).

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Gene therapy for human genetic diseases has not been successful yet.

Which of the following is common to both Sanger sequencing and next-generation sequencing?

Which of the following items is NOT a part of the name of a restriction enzyme?

________ are used in the detection of DNA by FISH.

The templates for next-generation sequencing are

Which of the following devices is used for PCR?

Which of the following microbes produces a protein that kills a variety of insect pests?

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

The natural role of restriction enzymes in bacteria is to

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Which of the following is essential in PCR?

Pest resistant cotton plants that produce Bt were produced by selective breeding.

Explain what a transgenic organism is,and give two examples.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic DNA Viruses

Pathogenic RNA Viruses

Applied and Environmental Microbiology

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