Question 1

Double strand DNA fragments are separated by size using

Accepted Answer

A)  restriction analysis. 
B)  FISH. 
C)  PCR. 
D)  northern blotting. 
E)  gel electrophoresis. 
A)  restriction analysis. 
B)  FISH. 
C)  PCR. 
D)  northern blotting. 
E)  gel electrophoresis.

Question 2

In gel electrophoresis,DNA molecules move toward the ________ electrode because they have an overall ________ charge.

Accepted Answer

A)  negative; negative 
B)  positive; positive 
C)  negative; positive 
D)  positive; negative 
E)  negative and positive; neutral 
A)  negative; negative 
B)  positive; positive 
C)  negative; positive 
D)  positive; negative 
E)  negative and positive; neutral

Question 3

Vectors usually contain genetic (markers/sequences/probes)such as antibiotic genes or fluorescent tags.

Accepted Answer

The answer of Vectors usually contain genetic (markers/sequences/probes)such as antibiotic...

Question 4

What is the function of the low temperature second step in PCR (polymerase chain reaction)?

Accepted Answer

A)  Activation of the DNA polymerase. 
B)  Allowing primers to bind complementary sequences. 
C)  Facilitate DNA synthesis. 
D)  Deactivate DNA polymerase to pause synthesis. 
E)  Facilitate the base pairing of deoxyribonucleotide triphosphates. 
A)  Activation of the DNA polymerase. 
B)  Allowing primers to bind complementary sequences. 
C)  Facilitate DNA synthesis. 
D)  Deactivate DNA polymerase to pause synthesis. 
E)  Facilitate the base pairing of deoxyribonucleotide triphosphates.

Question 5

Crop plants modified to be resistant to insect damage produce Bt toxin from a (bacterial/fungal/synthetic)gene.

Accepted Answer

The answer of Crop plants modified to be resistant to...

Question 6

The insertion of foreign DNA directly into a cell's nucleus using a glass micropipet is called (microporation/electroporation/microinjection).

Accepted Answer

The answer of The insertion of foreign DNA directly into...

Question 7

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Accepted Answer

A) True 
 B)False

Question 8

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

Accepted Answer

The answer of Short nucleic acid molecules used to locate...

Question 9

The goals of recombinant DNA technology include production of new organisms with useful combinations of traits.

Accepted Answer

A) True 
 B)False

Question 10

The technique known as real-time PCR is used to

Accepted Answer

A)  prepare cDNA molecules for cloning. 
B)  detect the presence of specific genetic sequences in a sample. 
C)  isolate DNA fragments for cloning. 
D)  measure the number of copies of a DNA sequence in a sample. 
E)  both detect and isolate DNA fragments in a sample. 
A)  prepare cDNA molecules for cloning. 
B)  detect the presence of specific genetic sequences in a sample. 
C)  isolate DNA fragments for cloning. 
D)  measure the number of copies of a DNA sequence in a sample. 
E)  both detect and isolate DNA fragments in a sample.

Question 11

Two men may be the father of a child.Figure 8.2 shows the results of a DNA fingerprint analysis to determine paternity.M is the mother,C is the child,A and B are the two men.What is the best interpretation of these results?

Accepted Answer

A)  The man identified as A is probably the father. 
B)  The man identified as B is probably the father. 
C)  Neither man is this child's father. 
D)  Either man could be this child's father. 
E)  Paternity cannot be determined from this data. 
A)  The man identified as A is probably the father. 
B)  The man identified as B is probably the father. 
C)  Neither man is this child's father. 
D)  Either man could be this child's father. 
E)  Paternity cannot be determined from this data.

Question 12

Which of the following is a product of recombinant DNA technology?

Accepted Answer

A)  rice that produces vitamin A 
B)  bacteria that produce antibiotics 
C)  yeast that produces alcohol 
D)  bacteria that produce acetone 
E)  microbes that digest crude oil 
A)  rice that produces vitamin A 
B)  bacteria that produce antibiotics 
C)  yeast that produces alcohol 
D)  bacteria that produce acetone 
E)  microbes that digest crude oil

Question 13

Outline a procedure for producing a useful new recombinant product.

Accepted Answer

Many variations are possible.The answer

Question 14

Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a

Accepted Answer

A)  primer. 
B)  probe. 
C)  plasmid. 
D)  promoter. 
E)  peptide. 
A)  primer. 
B)  probe. 
C)  plasmid. 
D)  promoter. 
E)  peptide.

Question 15

A northern blot differs from a Southern blot in the

Accepted Answer

A)  type of probe used. 
B)  presence or absence of a nitrocellulose membrane. 
C)  size of the genetic sequences involved. 
D)  number of genetic sequences detected. 
E)  type of nucleic acid being isolated. 
A)  type of probe used. 
B)  presence or absence of a nitrocellulose membrane. 
C)  size of the genetic sequences involved. 
D)  number of genetic sequences detected. 
E)  type of nucleic acid being isolated.

Question 16

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Accepted Answer

A)  Southern blotting 
B)  electroporation 
C)  gene gun 
D)  microinjection 
E)  protoplast fusion 
A)  Southern blotting 
B)  electroporation 
C)  gene gun 
D)  microinjection 
E)  protoplast fusion

Question 17

The technique of ________ is a means of determining the function of a gene by the phenotype resulting from its absence.

Accepted Answer

A)  gene therapy 
B)  gene knockout 
C)  probe analysis 
D)  expression vector cloning 
E)  FISH 
A)  gene therapy 
B)  gene knockout 
C)  probe analysis 
D)  expression vector cloning 
E)  FISH

Question 18

Mutagens are useful in biotechnology research for

Accepted Answer

A)  producing organisms with altered phenotypes. 
B)  producing new organisms which have beneficial traits from two or more organisms. 
C)  selecting genetic mutants resistant to radioactivity. 
D)  removing undesirable traits from microbes. 
E)  producing DNA fragments for cloning. 
A)  producing organisms with altered phenotypes. 
B)  producing new organisms which have beneficial traits from two or more organisms. 
C)  selecting genetic mutants resistant to radioactivity. 
D)  removing undesirable traits from microbes. 
E)  producing DNA fragments for cloning.

Question 19

The sequencing and analysis of an organism's genetic information is called

Accepted Answer

A)  protein synthesis. 
B)  gene therapy. 
C)  genomics. 
D)  northern blotting. 
E)  PCR. 
A)  protein synthesis. 
B)  gene therapy. 
C)  genomics. 
D)  northern blotting. 
E)  PCR.

Question 20

Subunit vaccines are safer than traditional vaccines because they

Accepted Answer

A)  are acellular. 
B)  are administered in food. 
C)  do not pose a risk for causing the disease. 
D)  are acellular and do not pose a risk for causing the disease. 
E)  are acellular and can be administered in food. 
A)  are acellular. 
B)  are administered in food. 
C)  do not pose a risk for causing the disease. 
D)  are acellular and do not pose a risk for causing the disease. 
E)  are acellular and can be administered in food.

Exam 8: Recombinant DNA Technology

Double strand DNA fragments are separated by size using

In gel electrophoresis,DNA molecules move toward the ________ electrode because they have an overall ________ charge.

Vectors usually contain genetic (markers/sequences/probes)such as antibiotic genes or fluorescent tags.

What is the function of the low temperature second step in PCR (polymerase chain reaction)?

Crop plants modified to be resistant to insect damage produce Bt toxin from a (bacterial/fungal/synthetic)gene.

The insertion of foreign DNA directly into a cell's nucleus using a glass micropipet is called (microporation/electroporation/microinjection).

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

The goals of recombinant DNA technology include production of new organisms with useful combinations of traits.

The technique known as real-time PCR is used to

Two men may be the father of a child.Figure 8.2 shows the results of a DNA fingerprint analysis to determine paternity.M is the mother,C is the child,A and B are the two men.What is the best interpretation of these results?

Which of the following is a product of recombinant DNA technology?

Outline a procedure for producing a useful new recombinant product.

Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a

A northern blot differs from a Southern blot in the

Which of the following procedures would be used to introduce DNA into a single mouse cell?

The technique of ________ is a means of determining the function of a gene by the phenotype resulting from its absence.

Mutagens are useful in biotechnology research for

The sequencing and analysis of an organism's genetic information is called

Subunit vaccines are safer than traditional vaccines because they

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic DNA Viruses

Pathogenic RNA Viruses

Applied and Environmental Microbiology

Filters

In gel electrophoresis,DNA molecules move toward the electrode because they have an overall charge.