Exam 2: The Structure of DNA

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As genome complexity increases, the time required for reannealing

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Use a graph to illustrate the effect of lowering the salt concentration of a solution on the melting temperature (Tm) of a DNA sample. What is the explanation for this relationship?

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Which form of DNA is predominant in cells - A, B, or Z DNA? Do other forms ever occur under special circumstances? Explain your answers.

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The DNA duplexes below are denatured and then allowed to reanneal. Which of the two molecules would have the highest Tm? Which of the two is least likely to re-form the original structure? Why? (a) 5′-ATATCATATGATATGTA-3′ 3′-TATAGTATACTATACAT-5′ (b) 5′-CGGTACTCGTGCAGGT-3′ 3′-GCCATGAGCACGTCCA-5′

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Which statement is not true about Z-DNA?

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