Question 1

Which of the following is NOT a useful feature of a plasmid for cloning?

Accepted Answer

A) expressed sequence tag 
B) a cloning site 
C) a replicator (origin of replication) 
D) a selectable marker 
A) expressed sequence tag 
B) a cloning site 
C) a replicator (origin of replication) 
D) a selectable marker

Question 2

What would be the 9-residue primer used to amplify this sequence?
5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

Accepted Answer

A) 5'-TAGCTGCAA-3' 
B) 5'-AAGCCTTAT-3' 
C) 5'-UAGCUGCAA-3' 
D) 5'-AAGCCUUAU-3' 
A) 5'-TAGCTGCAA-3' 
B) 5'-AAGCCTTAT-3' 
C) 5'-UAGCUGCAA-3' 
D) 5'-AAGCCUUAU-3'

Question 3

In the Southern hybridization procedure,after electrophoresis the gel is treated with NaOH and then neutralized before blotting.What is the primary function of the alkaline treatment?

Accepted Answer

A) to cleave the DNA into smaller fragments to permit greater efficiency of transfer 
B) to inactivate any restriction endonucleases that may be in the gel 
C) to denature the duplex DNA to single-stranded DNA (ssDN
A)  
D) to neutralize any acidic phosphate groups that might prevent hybridization 
A) to cleave the DNA into smaller fragments to permit greater efficiency of transfer 
B) to inactivate any restriction endonucleases that may be in the gel 
C) to denature the duplex DNA to single-stranded DNA (ssDN
A)  
D) to neutralize any acidic phosphate groups that might prevent hybridization

Question 4

What are linkers?

Accepted Answer

A) short sequences of DNA between the promoter and the gene 
B) short synthetic DNA duplexes consisting of a restriction site that is ligated onto DNA 
C) enzymes that link cloning sites in a sequence of nucleotides 
D) enzymes that incorporate foreign DNA into plasmids 
A) short sequences of DNA between the promoter and the gene 
B) short synthetic DNA duplexes consisting of a restriction site that is ligated onto DNA 
C) enzymes that link cloning sites in a sequence of nucleotides 
D) enzymes that incorporate foreign DNA into plasmids

Question 5

Reporter genes such as those for green fluorescent protein (GFP)are found on many commercial plasmids.What are these genes used for?

Accepted Answer

A) to determine if the plasmid is in the host 
B) to determine if a gene is present in a library 
C) to determine if a foreign protein is being expressed on a vector 
D) to determine the relative strength of a promoter sequence 
A) to determine if the plasmid is in the host 
B) to determine if a gene is present in a library 
C) to determine if a foreign protein is being expressed on a vector 
D) to determine the relative strength of a promoter sequence

Question 6

Which of the following is NOT correct for cDNA libraries?

Accepted Answer

A) Reverse transcriptase synthesizes a DNA strand complement of the mRNA templates. 
B) mRNA templates are isolated using oligo (d
A) -cellulose chromatography. 
C) Linkers are added and the cDNA is cloned into suitable vectors. 
D) The cDNA are copies from mRNA templates. 
A) Reverse transcriptase synthesizes a DNA strand complement of the mRNA templates. 
B) mRNA templates are isolated using oligo (d
A) -cellulose chromatography. 
C) Linkers are added and the cDNA is cloned into suitable vectors. 
D) The cDNA are copies from mRNA templates.

Question 7

Which of the following is NOT a part of the process associated with colony hybridization experiments?

Accepted Answer

A) A replica of the bacterial colonies is obtained on an absorbent disc. 
B) Autoradiography of the disc reveals probe-complementary DNA. 
C) Host bacteria with plasmid are plated and allowed to grow without media for days. 
D) The disc is treated with alkali. 
A) A replica of the bacterial colonies is obtained on an absorbent disc. 
B) Autoradiography of the disc reveals probe-complementary DNA. 
C) Host bacteria with plasmid are plated and allowed to grow without media for days. 
D) The disc is treated with alkali.

Question 8

Which of the following is a property of shuttle vectors?

Accepted Answer

A) They contain promoters for the expression of the gene. 
B) They have origins of replication for 2 different cell types,usually bacteria and yeast. 
C) They are capable of incorporating very large DNA fragments. 
D) They contain more than 1 antibiotic-resistant gene. 
A) They contain promoters for the expression of the gene. 
B) They have origins of replication for 2 different cell types,usually bacteria and yeast. 
C) They are capable of incorporating very large DNA fragments. 
D) They contain more than 1 antibiotic-resistant gene.

Question 9

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Accepted Answer

A) E.coli 
B) bacteriophage T4 
C) Thermus aquaticus 
D) Drosophila melanogaster 
A) E.coli 
B) bacteriophage T4 
C) Thermus aquaticus 
D) Drosophila melanogaster

Question 10

Which of the following is NOT a part of the procedure associated with the Southern blotting (hybridization)technique?

Accepted Answer

A) hybridization with radioactive probe 
B) agarose gel electrophoresis and visualize bands 
C) transfer (blot)to nitrocellulose filter 
D) digestion of DNA with DNA ligase 
A) hybridization with radioactive probe 
B) agarose gel electrophoresis and visualize bands 
C) transfer (blot)to nitrocellulose filter 
D) digestion of DNA with DNA ligase

Exam 27: Recombinant Dna: Cloning and Creation of Chimeric Genes

Which of the following is NOT a useful feature of a plasmid for cloning?

What would be the 9-residue primer used to amplify this sequence? 5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

In the Southern hybridization procedure,after electrophoresis the gel is treated with NaOH and then neutralized before blotting.What is the primary function of the alkaline treatment?

What are linkers?

Reporter genes such as those for green fluorescent protein (GFP)are found on many commercial plasmids.What are these genes used for?

Which of the following is NOT correct for cDNA libraries?

Which of the following is NOT a part of the process associated with colony hybridization experiments?

Which of the following is a property of shuttle vectors?

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Which of the following is NOT a part of the procedure associated with the Southern blotting (hybridization)technique?

The Facts of Life: Chemistry Is the Logic of Biological Phenomena

Water: the Medium of Life

Thermodynamics of Biological Systems

Amino Acids

Proteins: Their Primary Structure and Biological Functions

Proteins: Secondary, tertiary, and Quaternary Structure

Carbohydrates and the Glycoconjugates of Cell Surfaces

Lipids

Lipid Biosynthesis

Membranes and Membrane Transport

The Reception and Transmission of Extracellular Information

Enzymes Kinetics and Specificity

Mechanisms of Enzyme Action

Enzyme Regulation

Molecular Motors

Nutrition and the Organization of Metabolism

Glycolysis

The Tricarboxylic Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Gluconeogenesis, glycogen Metabolism, and the Pentose Phosphate Pathway

Fatty Acid Catabolism

Nitrogen Acquisition and Amino Acid Metabolism

Nucleotides and Nucleic Acids

Structure of Nucleic Acids

Synthesis and Degradation of Nucleotides

DNA Metabolism: Replication, recombination, and Repair

Transcription and the Regulation of Gene Expression

Protein Synthesis

Post-Transcriptional Regulation of Gene Expression

Completing the Protein Life Cycle: Folding, processing, and Degradation

Metabolic Integration and Organ Specialization

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