Exam 18: Control of Gene Expression in Eukaryotes

What is a key property of DNase that makes it useful for assessing whether chromatin is in a closed (tightly condensed)or open (loosely packed)configuration?

If DNA were inflexible,which of the following would not function?

Histone acetyl transferases exert their effect on gene activity by _____.

If natural selection over the time period of Eukaryote evolution has favored a DNA architecture in Eukaryotes that keeps genes turned off except when needed,this structure would be _____.

Which of the following is most critical for the association between histones and DNA?

If you extracted mRNA from the following,which would show the most differences in the sequences of mRNA found?

A gene is a _____.

Recently,the theobromine synthesis production of coffee plants was knocked down by the insertion of a gene that produced complementary hairpin mRNA.The process that led to these decaffeinated coffee plants is called _____.

Elsewhere in the paper,Chen et al.state that "BDNF is encoded by a complex gene with four well-characterized promoters that give rise to at least eight different mRNAs." What mechanism could account for the production of these different BDNF mRNAs from the same gene?

The reason for differences in the sets of proteins expressed in a nerve and a pancreatic cell of the same individual is that nerve and pancreatic cells contain different _____.

If cells of an individual contain the same set of genes,how do these cells become different from each other during development?

Which method is utilized by eukaryotes to control their gene expression that is not used in bacteria?

One way to detect alternative splicing of transcripts from a given gene is to _____.

Regulatory transcription factors _____.

Twenty-five years ago,when Oshima and colleagues discovered that a mutation in the GAL4 gene led to the inability to synthesize all five enzymes required for galactose catabolism (breakdown),they couldn't be blamed for wanting to apply a bacterial model to explain this finding.What they expected,but did not find,was _____.

If a pharmaceutical company wishes to make a protein-based drug from yeast with a particular carbohydrate tag to ensure correct tissue delivery,which stage of gene expression would be altered to change the tag on the protein?

In the roundworm C.elegans,the lin-4 gene produces an RNA that forms a hairpin structure.One of the strands in the double-stranded region of lin-4 hairpin RNA is complementary to the mRNA of a protein-coding gene,lin-14.Predict the effect of expressing lin-4 RNA during development.