Question 1

If you have inserted a gene in the Ti plasmid,the next step in genetic engineering is

Accepted Answer

A) transformation of E.coli with Ti plasmid. 
B) splicing T DNA into a plasmid. 
C) transformation of an animal cell. 
D) inserting the Ti plasmid into Agrobacterium. 
E) inserting the Ti plasmid into a plant cell. 
A) transformation of E.coli with Ti plasmid. 
B) splicing T DNA into a plasmid. 
C) transformation of an animal cell. 
D) inserting the Ti plasmid into Agrobacterium. 
E) inserting the Ti plasmid into a plant cell.

Question 2

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Accepted Answer

A) True 
 B)False

Question 3

Figure 9.5
  
-In Figure 9.5,the marker genes used for selecting recombinant DNA are

Accepted Answer

A) HindIII,BamHI,and EcoRI. 
B) ampᴿ and lacZ. 
C) ori. 
D) ampᴿ and ori. 
E) lacZ and ori. 
A) HindIII,BamHI,and EcoRI. 
B) ampᴿ and lacZ. 
C) ori. 
D) ampᴿ and ori. 
E) lacZ and ori.

Question 4

The field of proteomics concerns itself with

Accepted Answer

A) identifying an organism that makes improved enzymes. 
B) discovering the function of genes from a genetic sequence. 
C) determining all of the proteins expressed by a cell. 
D) synthesizing proteins from altered genetic sequences. 
E) identifying pathogens using RFLPs. 
A) identifying an organism that makes improved enzymes. 
B) discovering the function of genes from a genetic sequence. 
C) determining all of the proteins expressed by a cell. 
D) synthesizing proteins from altered genetic sequences. 
E) identifying pathogens using RFLPs.

Question 5

The following steps must be performed to make a bacterium produce human protein X.
1-Translation
2-Restriction enzyme
3-Prokaryotic transcription
4-DNA ligase
5-Transformation
6-Eukaryotic transcription
7-Reverse transcription
Which of the following places the steps in the correct order?

Accepted Answer

A) 5,2,3,4,7,6,1 
B) 1,2,3,5,4,7,6 
C) 6,7,2,3,4,5,1 
D) 6,7,2,4,5,3,1 
E) 6,2,1,3,4,5,7 
A) 5,2,3,4,7,6,1 
B) 1,2,3,5,4,7,6 
C) 6,7,2,3,4,5,1 
D) 6,7,2,4,5,3,1 
E) 6,2,1,3,4,5,7

Question 6

The reaction catalyzed by reverse transcriptase is

Accepted Answer

A) DNA → mRNA. 
B) mRNA → cDNA. 
C) mRNA → protein. 
D) DNA → DNA. 
E) tRNA → mRNA. 
A) DNA → mRNA. 
B) mRNA → cDNA. 
C) mRNA → protein. 
D) DNA → DNA. 
E) tRNA → mRNA.

Question 7

An advantage of using Saccharomyces cerevisiae over Escherichia coli to produce a human protein is that

Accepted Answer

A) it will be more likely to continuously secrete the protein. 
B) it will be less likely to express the protein. 
C) it has a genome only 4 times larger than E.coli. 
D) its genome is well understood unlike E.coli. 
E) it can be engineered to produce human proteins unlike E.coli. 
A) it will be more likely to continuously secrete the protein. 
B) it will be less likely to express the protein. 
C) it has a genome only 4 times larger than E.coli. 
D) its genome is well understood unlike E.coli. 
E) it can be engineered to produce human proteins unlike E.coli.

Question 8

111111
-Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC
A)

B)

C)

D)

E)

Accepted Answer

The answer of 111111
-Which enzyme would cut this strand of...

Question 9

The restriction enzyme EcoRI recognizes the sequence G↓AATTC.Which of the following is TRUE of DNA after it is treated with EcoRI?

Accepted Answer

A) All of the DNA fragments will have single-stranded regions ending in AA. 
B) All of the DNA fragments will have single-stranded regions ending in G. 
C) Some of the DNA will have single-stranded regions ending in AA and others will end in G. 
D) All of the DNA will have blunt ends. 
E) All of the DNA will be circular. 
A) All of the DNA fragments will have single-stranded regions ending in AA. 
B) All of the DNA fragments will have single-stranded regions ending in G. 
C) Some of the DNA will have single-stranded regions ending in AA and others will end in G. 
D) All of the DNA will have blunt ends. 
E) All of the DNA will be circular.

Question 10

Nearly all cells,including E.coli and yeast,naturally take up DNA from their surroundings without chemical treatment.

Accepted Answer

A) True 
 B)False

Question 11

An advantage of cDNA over genomic DNA is that it

Accepted Answer

A) lacks exons. 
B) lacks introns. 
C) contains selectable markers. 
D) can form very large DNA segments. 
E) is very easy to isolate. 
A) lacks exons. 
B) lacks introns. 
C) contains selectable markers. 
D) can form very large DNA segments. 
E) is very easy to isolate.

Question 12

The Human Genome Project,which was completed in 2003,was focused on

Accepted Answer

A) identifying all of the genes in the human genome. 
B) determining the nucleotide sequence of the entire human genome. 
C) determining all of the proteins encoded by the human genome. 
D) finding a cure for all human genetic disorders. 
E) cloning all of the genes of the human genome. 
A) identifying all of the genes in the human genome. 
B) determining the nucleotide sequence of the entire human genome. 
C) determining all of the proteins encoded by the human genome. 
D) finding a cure for all human genetic disorders. 
E) cloning all of the genes of the human genome.

Question 13

In recombinant DNA technology,a vector is a self-replicating segment of DNA,such as a plasmid or viral genome.

Accepted Answer

A) True 
 B)False

Question 14

Figure 9.5
  
-Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?

Accepted Answer

A) microinjection 
B) transformation 
C) gene guns 
D) Ti plasmids and Agrobacterium 
A) microinjection 
B) transformation 
C) gene guns 
D) Ti plasmids and Agrobacterium

Question 15

Your research group is investigating the possible use of genetically-engineered cells to produce a vaccine for malaria.List the pros and cons of using E.coli,Saccharomyces,and animal cells to produce the vaccine.

Accepted Answer

Pros and cons of using E. coli, Saccharo

Question 16

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Accepted Answer

A) gene guns 
B) protoplast fusion 
C) Ti plasmids and Agrobacterium 
D) microinjection 
E) electroporation 
A) gene guns 
B) protoplast fusion 
C) Ti plasmids and Agrobacterium 
D) microinjection 
E) electroporation

Question 17

Which of the following places the steps in the PCR procedure in the correct order?
1)Incubate at 94°C to denature DNA strands;
2)Incubate at 72°C for DNA synthesis;
3)Incubate at 60°C for primer hybridization.

Accepted Answer

A) 1,2,3 
B) 3,2,1 
C) 1,3,2 
D) 2;1;3 
E) 3;1;2 
A) 1,2,3 
B) 3,2,1 
C) 1,3,2 
D) 2;1;3 
E) 3;1;2

Question 18

You have a small gene that you wish replicated by PCR.After 3 replication cycles,how many double-stranded DNA molecules do you have?

Accepted Answer

A) 2 
B) 4 
C) 8 
D) 16 
E) thousands 
A) 2 
B) 4 
C) 8 
D) 16 
E) thousands

Question 19

Biotechnology involves the

Accepted Answer

A) use of microorganisms to make desired products. 
B) use of animal cells to make vaccines. 
C) development of disease-resistant crop plants. 
D) use of microorganisms to make desired products and the use of animal cells to make vaccines. 
E) use of microorganisms to make desired products,the use of animal cells to make vaccines,and the development of disease-resistant crop plants. 
A) use of microorganisms to make desired products. 
B) use of animal cells to make vaccines. 
C) development of disease-resistant crop plants. 
D) use of microorganisms to make desired products and the use of animal cells to make vaccines. 
E) use of microorganisms to make desired products,the use of animal cells to make vaccines,and the development of disease-resistant crop plants.

Question 20

The local public health agency has received reports of an outbreak of Salmonella gastroenteritis among attendees at a city-sponsored chili cook-off.What techniques from recombinant DNA technology would the agency likely use to investigate this outbreak? Describe the expected results from these techniques.

Accepted Answer

The local public health agency would lik

Exam 9: Biotechnology and Dna Technology

If you have inserted a gene in the Ti plasmid,the next step in genetic engineering is

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Figure 9.5 -In Figure 9.5,the marker genes used for selecting recombinant DNA are

The field of proteomics concerns itself with

The following steps must be performed to make a bacterium produce human protein X. 1-Translation 2-Restriction enzyme 3-Prokaryotic transcription 4-DNA ligase 5-Transformation 6-Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

The reaction catalyzed by reverse transcriptase is

An advantage of using Saccharomyces cerevisiae over Escherichia coli to produce a human protein is that

111111 -Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC A) B) C) D) E)

The restriction enzyme EcoRI recognizes the sequence G↓AATTC.Which of the following is TRUE of DNA after it is treated with EcoRI?

Nearly all cells,including E.coli and yeast,naturally take up DNA from their surroundings without chemical treatment.

An advantage of cDNA over genomic DNA is that it

The Human Genome Project,which was completed in 2003,was focused on

In recombinant DNA technology,a vector is a self-replicating segment of DNA,such as a plasmid or viral genome.

Figure 9.5 -Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?

Your research group is investigating the possible use of genetically-engineered cells to produce a vaccine for malaria.List the pros and cons of using E.coli,Saccharomyces,and animal cells to produce the vaccine.

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Which of the following places the steps in the PCR procedure in the correct order? 1)Incubate at 94°C to denature DNA strands; 2)Incubate at 72°C for DNA synthesis; 3)Incubate at 60°C for primer hybridization.

You have a small gene that you wish replicated by PCR.After 3 replication cycles,how many double-stranded DNA molecules do you have?

Biotechnology involves the

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, algae, protozoa, and Helminths

Viruses,viroids,and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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