Question 1

One of the first commercial successes of recombinant DNA technology was the production of human insulin using genetically engineered E.coli.

Accepted Answer

A) True 
 B)False

Question 2

PCR can be used to identify an unknown bacterium because

Accepted Answer

A) the DNA primer is specific to particular target DNA sequences. 
B) DNA polymerase will replicate any bacterial DNA. 
C) DNA can be electrophoresed. 
D) all cells have DNA. 
E) all cells have RNA. 
A) the DNA primer is specific to particular target DNA sequences. 
B) DNA polymerase will replicate any bacterial DNA. 
C) DNA can be electrophoresed. 
D) all cells have DNA. 
E) all cells have RNA.

Question 3

Choose the best pair to complete the following sentence.While ________ is/are responsible for the diversity of life,________ is/are responsible for shaping a population with organisms possessing characteristics that enhance survival.

Accepted Answer

A) vectors;rDNA. 
B) microbes;DNA. 
C) properties;vectors. 
D) selection;mutations. 
E) mutations;selection. 
A) vectors;rDNA. 
B) microbes;DNA. 
C) properties;vectors. 
D) selection;mutations. 
E) mutations;selection.

Question 4

The use of "suicide" genes in genetically modified organisms is designed to

Accepted Answer

A) prevent the growth of the modified organisms in the environment. 
B) kill the modified organisms before they are released in the environment. 
C) delete genes necessary for modified organism's growth. 
D) provide for resistance of the modified organisms to pesticides. 
E) provide a means to eliminate non-modified organisms. 
A) prevent the growth of the modified organisms in the environment. 
B) kill the modified organisms before they are released in the environment. 
C) delete genes necessary for modified organism's growth. 
D) provide for resistance of the modified organisms to pesticides. 
E) provide a means to eliminate non-modified organisms.

Question 5

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Accepted Answer

A) self-replication 
B) large size 
C) has a selectable marker 
D) circular form of DNA or integrates into the host chromosome 
E) may replicate in several species 
A) self-replication 
B) large size 
C) has a selectable marker 
D) circular form of DNA or integrates into the host chromosome 
E) may replicate in several species

Question 6

Which of the following is NOT an agricultural product made by DNA techniques?

Accepted Answer

A) frost retardant 
B) Bacillus thuringiensis insecticide 
C) nitrogenase (nitrogen fixation) 
D) glyphosate-resistant crops 
E) pectinase 
A) frost retardant 
B) Bacillus thuringiensis insecticide 
C) nitrogenase (nitrogen fixation) 
D) glyphosate-resistant crops 
E) pectinase

Question 7

A population of cells carrying a desired plasmid is called a

Accepted Answer

A) library. 
B) clone. 
C) vector. 
D) Southern blot. 
E) PCR. 
A) library. 
B) clone. 
C) vector. 
D) Southern blot. 
E) PCR.

Question 8

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases,such as hepatitis B?

Accepted Answer

A) RNA interference (RNAi) 
B) complementary DNA (cDN
A)  
C) reverse transcriptase PCR (rtPCR) 
D) tumor-inducing plasmids (Ti plasmids) 
E) DNA fingerprinting 
A) RNA interference (RNAi) 
B) complementary DNA (cDN
A)  
C) reverse transcriptase PCR (rtPCR) 
D) tumor-inducing plasmids (Ti plasmids) 
E) DNA fingerprinting

Question 9

One advantage of a viral vector over a plasmid vector is that the

Accepted Answer

A) viral vector can accept much larger pieces of DNA. 
B) plasmid vector is circular. 
C) viral vector can accept a PCR fragment. 
D) viral vector can harbor a selection marker. 
E) the viral vector can accept larger pieces of DNA and the plasmid vector is circular. 
A) viral vector can accept much larger pieces of DNA. 
B) plasmid vector is circular. 
C) viral vector can accept a PCR fragment. 
D) viral vector can harbor a selection marker. 
E) the viral vector can accept larger pieces of DNA and the plasmid vector is circular.

Question 10

Pseudomonas syringae is found naturally in the soil.Sold as Snomax,it is used to make snow at ski resorts.The same bacterium with a gene deletion (Ice-minus)is used to prevent ice formation on plants.Should Snomax and Ice-minus be considered modified organisms and subject to precautions of releasing genetically modified organisms? Explain why or why not.

Accepted Answer

Yes, both Snomax and Ice-minus should be

Question 11

A shuttle vector is a plasmid that is used to move pieces of DNA among organisms,such as bacterial,fungal,and plant cells.

Accepted Answer

A) True 
 B)False

Question 12

A source of heat-stable DNA polymerase is

Accepted Answer

A) Agrobacterium tumefaciens. 
B) Thermus aquaticus. 
C) Saccharomyces cerevisiae. 
D) Bacillus thuringiensis. 
E) Pseudomonas. 
A) Agrobacterium tumefaciens. 
B) Thermus aquaticus. 
C) Saccharomyces cerevisiae. 
D) Bacillus thuringiensis. 
E) Pseudomonas.

Question 13

The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells.A new,rapid diagnostic test to detect human papilloma virus (HPV)DNA before cancer develops is done without microscopic exam.The steps involved in this FastHPV test are listed below.What is the second step?

Accepted Answer

A) Add an RNA probe for HPV DNA. 
B) Lyse human cells. 
C) Add enzyme-linked antibodies against DNA-RNA. 
D) Add enzyme substrate. 
E) The order is unimportant. 
A) Add an RNA probe for HPV DNA. 
B) Lyse human cells. 
C) Add enzyme-linked antibodies against DNA-RNA. 
D) Add enzyme substrate. 
E) The order is unimportant.

Question 14

Restriction enzymes function as

Accepted Answer

A) bacterial enzymes that splice DNA. 
B) bacterial enzymes that destroy phage DNA. 
C) animal enzymes that splice RNA. 
D) viral enzymes that destroy host DNA. 
A) bacterial enzymes that splice DNA. 
B) bacterial enzymes that destroy phage DNA. 
C) animal enzymes that splice RNA. 
D) viral enzymes that destroy host DNA.

Question 15

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Accepted Answer

A) DNA fingerprints 
B) restriction fragment length polymorphisms 
C) reverse-transcriptase PCR (rtPCR) 
D) DNA fingerprints and restriction fragment length polymorphisms 
E) DNA fingerprints,restriction fragment length polymorphisms,and reverse-transcriptase PCR(rtPCR) 
A) DNA fingerprints 
B) restriction fragment length polymorphisms 
C) reverse-transcriptase PCR (rtPCR) 
D) DNA fingerprints and restriction fragment length polymorphisms 
E) DNA fingerprints,restriction fragment length polymorphisms,and reverse-transcriptase PCR(rtPCR)

Question 16

Self-replicating DNA used to transmit a gene from one organism to another is a

Accepted Answer

A) library. 
B) clone. 
C) vector. 
D) Southern blot. 
E) PCR. 
A) library. 
B) clone. 
C) vector. 
D) Southern blot. 
E) PCR.

Question 17

Figure 9.5
  
-In Figure 9.5,the gene that allows the plasmid to be self-replicating is

Accepted Answer

A) HindIII. 
B) ampᴿ. 
C) ori. 
D) EcoRI. 
E) lacZ. 
A) HindIII. 
B) ampᴿ. 
C) ori. 
D) EcoRI. 
E) lacZ.

Question 18

The random shotgun method is used in

Accepted Answer

A) amplification of unknown DNA. 
B) transforming plant cells with recombinant DNA. 
C) genome sequencing. 
D) RFLP analysis. 
E) forensic microbiology. 
A) amplification of unknown DNA. 
B) transforming plant cells with recombinant DNA. 
C) genome sequencing. 
D) RFLP analysis. 
E) forensic microbiology.

Question 19

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

Accepted Answer

A) True 
 B)False

Question 20

The practice of breeding plants and animals for desirable traits,such as high crop yield,is called natural selection.

Accepted Answer

A) True 
 B)False

Exam 9: Biotechnology and Dna Technology

One of the first commercial successes of recombinant DNA technology was the production of human insulin using genetically engineered E.coli.

PCR can be used to identify an unknown bacterium because

Choose the best pair to complete the following sentence.While ________ is/are responsible for the diversity of life,________ is/are responsible for shaping a population with organisms possessing characteristics that enhance survival.

The use of "suicide" genes in genetically modified organisms is designed to

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Which of the following is NOT an agricultural product made by DNA techniques?

A population of cells carrying a desired plasmid is called a

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases,such as hepatitis B?

One advantage of a viral vector over a plasmid vector is that the

A shuttle vector is a plasmid that is used to move pieces of DNA among organisms,such as bacterial,fungal,and plant cells.

A source of heat-stable DNA polymerase is

Restriction enzymes function as

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Self-replicating DNA used to transmit a gene from one organism to another is a

Figure 9.5 -In Figure 9.5,the gene that allows the plasmid to be self-replicating is

The random shotgun method is used in

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

The practice of breeding plants and animals for desirable traits,such as high crop yield,is called natural selection.

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, algae, protozoa, and Helminths

Viruses,viroids,and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Filters

Choose the best pair to complete the following sentence.While is/are responsible for the diversity of life, is/are responsible for shaping a population with organisms possessing characteristics that enhance survival.