Question 1

Standard precautions include:

Accepted Answer

A)  using barrier devices to prevent exposure to skin and mucous membranes. 
B)  recapping needles by hand. 
C)  storing drinks but not food in laboratory refrigerators. 
D)  discarding sharps in plastic biohazard bags. 
A)  using barrier devices to prevent exposure to skin and mucous membranes. 
B)  recapping needles by hand. 
C)  storing drinks but not food in laboratory refrigerators. 
D)  discarding sharps in plastic biohazard bags. A

Question 2

Optimal protection of specimen, personnel, and environment is accomplished with which class of biological safety cabinet?

Accepted Answer

A)  Class I 
B)  Class II 
C)  Class III 
D)  Class IV 
A)  Class I 
B)  Class II 
C)  Class III 
D)  Class IV C

Question 3

The agency that has published guidelines known as standard precautions to reduce the risk of disease transmission in laboratories is:

Accepted Answer

A)  DHEC. 
B)  OSHA. 
C)  CDC. 
D)  EPA. 
A)  DHEC. 
B)  OSHA. 
C)  CDC. 
D)  EPA. C

Question 4

A cleaning solution used on the venipuncture site to avoid skin contamination of the blood sample is called a(n):

Accepted Answer

A)  surfactant. 
B)  disinfectant. 
C)  biocide. 
D)  antiseptic. 
A)  surfactant. 
B)  disinfectant. 
C)  biocide. 
D)  antiseptic.

Question 5

A cleaning solution used on the laboratory bench top to kill bacteria that are potentially harmful to humans would be called a(n):

Accepted Answer

A)  surfactant. 
B)  disinfectant. 
C)  biocide. 
D)  antiseptic. 
A)  surfactant. 
B)  disinfectant. 
C)  biocide. 
D)  antiseptic.

Question 6

Minimal protection of specimen, personnel, and environment is accomplished with which class of biological safety cabinet?

Accepted Answer

A)  Class I 
B)  Class II 
C)  Class III 
D)  Class IV 
A)  Class I 
B)  Class II 
C)  Class III 
D)  Class IV

Question 7

The agency that requires health care facilities to provide employees with all devices and mechanisms necessary to protect them from the hazards encountered in the laboratory is:

Accepted Answer

A)  Department of Health and Environmental Control (DHE
C) . 
B)  Occupational Safety and Health Administration (OSH
A) . 
C)  Centers for Disease Control and Prevention (CD
C) . 
D)  Health Emergency Planning Advisor (HEP
A) . 
A)  Department of Health and Environmental Control (DHE
C) . 
B)  Occupational Safety and Health Administration (OSH
A) . 
C)  Centers for Disease Control and Prevention (CD
C) . 
D)  Health Emergency Planning Advisor (HEP
A) .

Question 8

Mouth pipetting:

Accepted Answer

A)  should be performed only if necessary and with extreme care. 
B)  can save valuable time and should be performed whenever quick results are needed. 
C)  is performed only when material is not expected to pose a biological hazard. 
D)  should not be performed in any circumstance in the clinical laboratory. 
A)  should be performed only if necessary and with extreme care. 
B)  can save valuable time and should be performed whenever quick results are needed. 
C)  is performed only when material is not expected to pose a biological hazard. 
D)  should not be performed in any circumstance in the clinical laboratory.

Question 9

Biosafety Level 1 agents:

Accepted Answer

A)  include those that are the common agents of an infectious disease. 
B)  require only standard good laboratory techniques. 
C)  include Mycobacterium tuberculosis, M. gordonae, and Brucella. 
D)  require the use of maximum containment facilities. 
A)  include those that are the common agents of an infectious disease. 
B)  require only standard good laboratory techniques. 
C)  include Mycobacterium tuberculosis, M. gordonae, and Brucella. 
D)  require the use of maximum containment facilities.

Question 10

Standard precautions in the microbiology laboratory apply to:

Accepted Answer

A)  all clinical specimens. 
B)  clinical specimens that contain blood or body fluids. 
C)  blood, body fluids, feces, sputum, saliva, urine, and vomitus. 
D)  only specimens that are visibly bloody. 
A)  all clinical specimens. 
B)  clinical specimens that contain blood or body fluids. 
C)  blood, body fluids, feces, sputum, saliva, urine, and vomitus. 
D)  only specimens that are visibly bloody.

Question 11

The most effective antiseptic agent against blood spills and therefore the antiseptic of choice in laboratories where these agents are likely found is:

Accepted Answer

A)  70% ethanol. 
B)  phenol compound. 
C)  absolute ethanol. 
D)  10% bleach. 
A)  70% ethanol. 
B)  phenol compound. 
C)  absolute ethanol. 
D)  10% bleach.

Question 12

Procedures such as grinding, mincing, vortexing, and preparing smears:

Accepted Answer

A)  are hazardous and should be performed while wearing gloves and gown protection. 
B)  create aerosol droplets and should be performed only in an approved biosafety cabinet. 
C)  should be avoided with specimens known to contain infectious agents. 
D)  produce a spill hazard and should be performed over an absorptive mat. 
A)  are hazardous and should be performed while wearing gloves and gown protection. 
B)  create aerosol droplets and should be performed only in an approved biosafety cabinet. 
C)  should be avoided with specimens known to contain infectious agents. 
D)  produce a spill hazard and should be performed over an absorptive mat.

Question 13

Working with biosafety Level 3 agents requires:

Accepted Answer

A)  U.S. Department of Agriculture (USD
A)  credentials and a postgraduate degree in microbiology or a related field. 
B)  laboratory design features that control air movement. 
C)  personnel to wear protective clothing and devices and enter a decontaminating chamber before leaving. 
D)  workers to have blood samples routinely tested to rule out infection or contamination. 
A)  U.S. Department of Agriculture (USD
A)  credentials and a postgraduate degree in microbiology or a related field. 
B)  laboratory design features that control air movement. 
C)  personnel to wear protective clothing and devices and enter a decontaminating chamber before leaving. 
D)  workers to have blood samples routinely tested to rule out infection or contamination.

Question 14

Class I biological safety cabinets:

Accepted Answer

A)  sterilize the air as it passes over the material in the cabinet. 
B)  protect the environment from contamination by biological agents by filtering the air as it is exhausted from the cabinet. 
C)  are completely enclosed and have gloves that enable the worker to manipulate the material in the cabinet. 
D)  are often called laminar flow hoods. 
A)  sterilize the air as it passes over the material in the cabinet. 
B)  protect the environment from contamination by biological agents by filtering the air as it is exhausted from the cabinet. 
C)  are completely enclosed and have gloves that enable the worker to manipulate the material in the cabinet. 
D)  are often called laminar flow hoods.

Question 15

The agency that has published guidelines regarding the packaging of biohazardous specimens is known as:

Accepted Answer

A)  DHEC. 
B)  International Air Transport Association (IAT
A) . 
C)  CDC. 
D)  EPA. 
A)  DHEC. 
B)  International Air Transport Association (IAT
A) . 
C)  CDC. 
D)  EPA.

Question 16

To be sterilized in an autoclave, media or instruments should be:

Accepted Answer

A)  packed tightly to avoid dangerous pressure pockets. 
B)  processed for 15 minutes at 121° C and 15 psi pressure. 
C)  processed for 30 to 60 minutes at 132° C and 15 psi pressure. 
D)  processed for at least 1 hour at 121° C and 15 psi pressure. 
A)  packed tightly to avoid dangerous pressure pockets. 
B)  processed for 15 minutes at 121° C and 15 psi pressure. 
C)  processed for 30 to 60 minutes at 132° C and 15 psi pressure. 
D)  processed for at least 1 hour at 121° C and 15 psi pressure.

Question 17

Biosafety Level 4 agents:

Accepted Answer

A)  include those that are the common agents of infectious disease. 
B)  require only standard good laboratory technique. 
C)  include Mycobacterium tuberculosis, M. gordonae, and Brucella. 
D)  require the use of maximum containment facilities. 
A)  include those that are the common agents of infectious disease. 
B)  require only standard good laboratory technique. 
C)  include Mycobacterium tuberculosis, M. gordonae, and Brucella. 
D)  require the use of maximum containment facilities.

Question 18

The method of choice for sterilizing items such as antibiotic solutions, chemicals, radioisotopes, and vaccines that are heat-sensitive is:

Accepted Answer

A)  dry, low temperature heat. 
B)  moist, low temperature heat. 
C)  filtration. 
D)  chemical. 
A)  dry, low temperature heat. 
B)  moist, low temperature heat. 
C)  filtration. 
D)  chemical.

Question 19

To be sterilized in an autoclave, infectious medical waste should be:

Accepted Answer

A)  packed tightly to avoid dangerous pressure pockets. 
B)  processed for 15 to 20 minutes at 121° C and 30 pounds per square inch (psi) pressure. 
C)  processed for 30 to 60 minutes at 132° C and 15 psi pressure. 
D)  processed for at least 1 hour at 121° C and 15 psi pressure. 
A)  packed tightly to avoid dangerous pressure pockets. 
B)  processed for 15 to 20 minutes at 121° C and 30 pounds per square inch (psi) pressure. 
C)  processed for 30 to 60 minutes at 132° C and 15 psi pressure. 
D)  processed for at least 1 hour at 121° C and 15 psi pressure.

Question 20

Biosafety Level 2 agents:

Accepted Answer

A)  include those that are the common agents of infectious disease. 
B)  require only standard good laboratory technique. 
C)  include Mycobacterium tuberculosis, M. gordonae, and Brucella. 
D)  require the use of maximum containment facilities. 
A)  include those that are the common agents of infectious disease. 
B)  require only standard good laboratory technique. 
C)  include Mycobacterium tuberculosis, M. gordonae, and Brucella. 
D)  require the use of maximum containment facilities.

Exam 4: Laboratory Safety

Standard precautions include:

Optimal protection of specimen, personnel, and environment is accomplished with which class of biological safety cabinet?

The agency that has published guidelines known as standard precautions to reduce the risk of disease transmission in laboratories is:

A cleaning solution used on the venipuncture site to avoid skin contamination of the blood sample is called a(n):

A cleaning solution used on the laboratory bench top to kill bacteria that are potentially harmful to humans would be called a(n):

Minimal protection of specimen, personnel, and environment is accomplished with which class of biological safety cabinet?

The agency that requires health care facilities to provide employees with all devices and mechanisms necessary to protect them from the hazards encountered in the laboratory is:

Mouth pipetting:

Biosafety Level 1 agents:

Standard precautions in the microbiology laboratory apply to:

The most effective antiseptic agent against blood spills and therefore the antiseptic of choice in laboratories where these agents are likely found is:

Procedures such as grinding, mincing, vortexing, and preparing smears:

Working with biosafety Level 3 agents requires:

Class I biological safety cabinets:

The agency that has published guidelines regarding the packaging of biohazardous specimens is known as:

To be sterilized in an autoclave, media or instruments should be:

Biosafety Level 4 agents:

The method of choice for sterilizing items such as antibiotic solutions, chemicals, radioisotopes, and vaccines that are heat-sensitive is:

To be sterilized in an autoclave, infectious medical waste should be:

Biosafety Level 2 agents:

Microbial Taxonomy

Bacterial Genetics, Metabolism, and Structure

Host-Microorganism Interactions

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action and Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus Micrococcus and Similar Organisms

Streptococcus Enterococcus and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix Lactobacillus and Similar Organisms

Nocardia Streptomyces Rhodococcus and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Chromobacterium, and Related Organisms

Sphingomonas Paucimobilis and Similar Organisms

Moraxella and Related Organisms

Eikenella and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis, Bordetella Parapertussis, and Related Species

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Overview of Anaerobic Organisms

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections: Overview

Intestinal Protozoa

Blood and Tissue Protozoa

Other Protozoa

Intestinal Nematodes Roundworms

Tissue Nematodes Roundworms

Blood and Tissue Filarialnematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Mucorales Zygomycetes, Dermatophytes, and Opportunitistic and Systemic Mycoses

Dematiaceous Melanizedmolds