Question 1

Rectal swab is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable. B

Question 2

Tissue biopsy taken from a puncture wound is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable. A

Question 3

Feces in a sterile specimen cup is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable. B

Question 4

MATCHING
Match the media with its primary purpose.
-CCFA

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 5

Urine via catheter is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable.

Question 6

Prereduced, anaerobically sterilized (PRAS)media:

Accepted Answer

A)  are produced, packaged, shipped, and stored under anaerobic conditions. 
B)  have a short shelf life of only 2 weeks. 
C)  enable the culture to be incubated in the regular incubator under aerobic conditions. 
D)  contain peroxides that enhance the growth of certain anaerobes. 
A)  are produced, packaged, shipped, and stored under anaerobic conditions. 
B)  have a short shelf life of only 2 weeks. 
C)  enable the culture to be incubated in the regular incubator under aerobic conditions. 
D)  contain peroxides that enhance the growth of certain anaerobes.

Question 7

Nasopharyngeal swab is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable.

Question 8

MATCHING
Match the media with its primary purpose.
-PEA agar

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 9

The indicator used to determine whether anaerobic conditions are achieved in an anaerobic jar is:

Accepted Answer

A)  phenol red. 
B)  bromothymol blue. 
C)  methylene blue. 
D)  methyl red. 
A)  phenol red. 
B)  bromothymol blue. 
C)  methylene blue. 
D)  methyl red.

Question 10

Aspiration of peritoneal fluid using a needle and syringe is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable.

Question 11

MATCHING
Match the media with its primary purpose.
-Egg-yolk agar

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 12

An anaerobic media, which contains ferric ammonium citrate and hemin, bile salts, and gentamicin, is used to isolate which group of organisms?

Accepted Answer

A)  Bacteroides spp. 
B)  Clostridia spp. 
C)  Actinomyces spp. 
D)  Veillonella spp. 
A)  Bacteroides spp. 
B)  Clostridia spp. 
C)  Actinomyces spp. 
D)  Veillonella spp.

Question 13

The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of:

Accepted Answer

A)  20% to 30% nitrogen, 5% to 10% hydrogen, and 70% to 80% CO2. 
B)  80% to 90% oxygen, 5% to 10% hydrogen, and 5% to 10% CO2. 
C)  80% to 90% nitrogen, 5% to 10% hydrogen, and 5% to 10% CO2. 
D)  80% to 90% nitrogen, 5% to 10% oxygen, and 5% to 10% CO2. 
A)  20% to 30% nitrogen, 5% to 10% hydrogen, and 70% to 80% CO2. 
B)  80% to 90% oxygen, 5% to 10% hydrogen, and 5% to 10% CO2. 
C)  80% to 90% nitrogen, 5% to 10% hydrogen, and 5% to 10% CO2. 
D)  80% to 90% nitrogen, 5% to 10% oxygen, and 5% to 10% CO2.

Question 14

Actinomyces spp., Bifidobacterium spp., and Clostridium spp.can be described as:

Accepted Answer

A)  aerotolerant organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under microaerobic conditions. 
B)  strictly anaerobic organisms that cannot grow in the presence of either reduced or atmospheric oxygen but grow only under anaerobic conditions. 
C)  microaerophilic organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under anaerobic conditions. 
D)  facultatively anaerobic organisms that grow in the presence or absence of atmospheric oxygen. 
A)  aerotolerant organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under microaerobic conditions. 
B)  strictly anaerobic organisms that cannot grow in the presence of either reduced or atmospheric oxygen but grow only under anaerobic conditions. 
C)  microaerophilic organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under anaerobic conditions. 
D)  facultatively anaerobic organisms that grow in the presence or absence of atmospheric oxygen.

Question 15

An acceptable specimen for anaerobic culture is:

Accepted Answer

A)  nasopharyngeal swab. 
B)  tissue obtained at biopsy. 
C)  small bowel contents. 
D)  catheterized urine. 
A)  nasopharyngeal swab. 
B)  tissue obtained at biopsy. 
C)  small bowel contents. 
D)  catheterized urine.

Question 16

Percutaneous lung aspirate is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable.

Question 17

To isolate anaerobic gram-positive organisms, which of the following media should be used?

Accepted Answer

A)  Anaerobic blood agar 
B)  Cycloserine cefoxitin fructose agar (CCF
A)  
C)  Laked kanamycin-vancomycin (LKV) blood agar 
D)  Anaerobic phenylethyl alcohol agar (PE
A)  
A)  Anaerobic blood agar 
B)  Cycloserine cefoxitin fructose agar (CCF
A)  
C)  Laked kanamycin-vancomycin (LKV) blood agar 
D)  Anaerobic phenylethyl alcohol agar (PE
A)

Question 18

Urine via percutaneous aspiration is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable.

Question 19

MATCHING
Match the media with its primary purpose.
-Thioglycollate broth

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective; supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 20

Expectorated sputum is:

Accepted Answer

A)  acceptable. 
B)  not acceptable. 
A)  acceptable. 
B)  not acceptable.

Exam 40: Overview and General Considerations

Rectal swab is:

Tissue biopsy taken from a puncture wound is:

Feces in a sterile specimen cup is:

MATCHING Match the media with its primary purpose. -CCFA

Urine via catheter is:

Prereduced, anaerobically sterilized (PRAS)media:

Nasopharyngeal swab is:

MATCHING Match the media with its primary purpose. -PEA agar

The indicator used to determine whether anaerobic conditions are achieved in an anaerobic jar is:

Aspiration of peritoneal fluid using a needle and syringe is:

MATCHING Match the media with its primary purpose. -Egg-yolk agar

An anaerobic media, which contains ferric ammonium citrate and hemin, bile salts, and gentamicin, is used to isolate which group of organisms?

The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of:

Actinomyces spp., Bifidobacterium spp., and Clostridium spp.can be described as:

An acceptable specimen for anaerobic culture is:

Percutaneous lung aspirate is:

To isolate anaerobic gram-positive organisms, which of the following media should be used?

Urine via percutaneous aspiration is:

MATCHING Match the media with its primary purpose. -Thioglycollate broth

Expectorated sputum is:

Microbial Taxonomy

Bacterial Genetics, Metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action and Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus Micrococcus and Similar Organisms

Streptococcus Enterococcus and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix Lactobacillus and Similar Organisms

Nocardia Streptomyces Rhodococcus and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Chromobacterium, and Related Organisms

Sphingomonas Paucimobilis and Similar Organisms

Moraxella and Related Organisms

Eikenella and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis, Bordetella Parapertussis, and Related Species

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview of Anaerobic Organisms

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections: Overview

Intestinal Protozoa

Blood and Tissue Protozoa

Other Protozoa

Intestinal Nematodes Roundworms

Tissue Nematodes Roundworms

Blood and Tissue Filarialnematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Mucorales Zygomycetes, Dermatophytes, and Opportunitistic and Systemic Mycoses

Dematiaceous Melanizedmolds