Exam 8: Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Complementary nucleic acid hybridization, which is coupled with nucleic acid replication and applied repeatedly through numerous cycles, is based on which of the following methods?

Determining the exact nucleotide sequence of a gene or gene fragment obtained from an unknown organism is based on which of the following methods?

Nucleic acid sequencing methods are based on:

The enzyme that has the ability to synthesize DNA from ribonucleic acid (RNA)is called:

Gen-Probe Mycobacterium tuberculosis direct test uses which type of nucleic acid amplification test?

The enzyme that is commonly used for primer extensions in the PCR method works best at what temperature?

Which enzyme recognizes a specific nucleotide sequence (~4-8 nucleotides in length)and catalyzes the digestion of the nucleic acid at that site, causing a break in the DNA strand?

The optimal way to detect PCR amplicons is to use which stain?

Hybridization methods are based on:

The probe in the hybridization assay can be described as:

Target nucleic acid amplification (polymerase chain reaction [PCR])methods are based on:

An organism whose genetic makeup is 50% C-G bonds would have a(n)_________ melting temperature (Tm), compared with an organism with 20% C-G bonds.

The ability of two nucleic acid strands, a probe and a target, which have complementary base sequences that specifically bond with each other and form a double-stranded molecule, drives which of the following methods?

The target in the hybridization assay can be described as:

Which type of hybridization method would be the optimal choice to use to detect a lesion infected with a particular virus?