Question 1

Which of the following can be used to express a second transgene from a single mRNA?

Accepted Answer

A)  Downstream AUG. 
B)  IRES 
C)  Alternative mRNA splicing signals. 
D)  RNA editing sequences 
E)  All of the above. 
A)  Downstream AUG. 
B)  IRES 
C)  Alternative mRNA splicing signals. 
D)  RNA editing sequences 
E)  All of the above.

Question 2

Which of the following is a major disadvantage of using a vector based on adeno-associated virus (AAV)rather than an adenovirus or a retrovirus?

Accepted Answer

A)  The DNA of the AAV vectors integrates into specific location in the genome. 
B)  The virus is toxic to human cells. 
C)  It infects cells of the immune system, causing a strong immune response. 
D)  The immune system reacts very strongly to the AAV proteins. 
E)  The small genome can only tolerate an insert of 4.5kb. 
A)  The DNA of the AAV vectors integrates into specific location in the genome. 
B)  The virus is toxic to human cells. 
C)  It infects cells of the immune system, causing a strong immune response. 
D)  The immune system reacts very strongly to the AAV proteins. 
E)  The small genome can only tolerate an insert of 4.5kb.

Question 3

SV40 was the first virus used to express a foreign protein in a cultured cell.But SV40 has which of the following limitations?

Accepted Answer

A)  The small genome can only accommodate a small gene. 
B)  It can only infect monkey cells. 
C)  It does not integrate its genome into the host cell genome. 
D)  It replicates very slowly. 
E)  It does not have a strong promoter/enhancer for gene expression. 
A)  The small genome can only accommodate a small gene. 
B)  It can only infect monkey cells. 
C)  It does not integrate its genome into the host cell genome. 
D)  It replicates very slowly. 
E)  It does not have a strong promoter/enhancer for gene expression.

Question 4

Since vectors based on lentiviruses can infect nondividing cells, they have an advantage over vectors based on the genomes of other retroviruses.

Accepted Answer

A) True 
 B)False

Question 5

RNA virus vectors do not require the same enhancer/promoter sequences as DNA virus vectors.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following viral proteins controls the types of cells that a retrovirus can infect?

Accepted Answer

A)  Capsid protein 
B)  Gag proteins 
C)  Protease 
D)  Envelope protein 
E)  Reverse transcriptase 
A)  Capsid protein 
B)  Gag proteins 
C)  Protease 
D)  Envelope protein 
E)  Reverse transcriptase

Exam 37: Eukaryotic Virus Vectors

Which of the following can be used to express a second transgene from a single mRNA?

Which of the following is a major disadvantage of using a vector based on adeno-associated virus (AAV)rather than an adenovirus or a retrovirus?

SV40 was the first virus used to express a foreign protein in a cultured cell.But SV40 has which of the following limitations?

Since vectors based on lentiviruses can infect nondividing cells, they have an advantage over vectors based on the genomes of other retroviruses.

RNA virus vectors do not require the same enhancer/promoter sequences as DNA virus vectors.

Which of the following viral proteins controls the types of cells that a retrovirus can infect?

Introduction to Virology

Virus Structure and Assembly

Virus Classification: The World of Viruses

Virus Entry

Single-Stranded RNA Bacteriophages

Microviruses

Bacteriophage

Bacteriophage Lambda

Viruses of Archaea

Cucumber Mosaic Virus

Picornaviruses

Flaviviruses

Togaviruses

Coronaviruses

Paramyxo-Viruses and Rhabdoviruses

Filovirouses

Bunyaviruses

Influenza Viruses

Reoviruses

Parvoviruses

Polyomaviruses

Papillomaviruses

Adenoviruses

Herpesviruses

Baculoviruses

Poxviruses

Viruses of Algae and Mimivirus

Retroviruses

Human Immunodeficiency

Hepadnaviruses

Viroids and Hepatitis Delta Virus

Prions

Intrinsic Cellular Defenses Against Virus Infection

Innate and Adaptive Immune Responses to Virus Infection

Antiviral Vaccines

Antiviral Chemotherapy

Filters