Question 1

The gene for human insulin has been successfully cloned in

Accepted Answer

A) S.aureus. 
B) rhinovirus. 
C) coli. 
A) S.aureus. 
B) rhinovirus. 
C) coli.

Question 2

The size of the amplified DNA fragment generated during PCR is determined by

Accepted Answer

A) how many cycles are performed. 
B) the size of the template DNA. 
C) the location to which the primers anneal. 
D) how much Taq is used. 
A) how many cycles are performed. 
B) the size of the template DNA. 
C) the location to which the primers anneal. 
D) how much Taq is used.

Question 3

Host cells containing recombinant DNA can be selected on the basis of the properties of the

Accepted Answer

A) vector. 
B) ribosomes. 
C) enzymes. 
D) virus. 
A) vector. 
B) ribosomes. 
C) enzymes. 
D) virus.

Question 4

The current cost of sequencing a human genome is

Accepted Answer

A) about $10 
B) about $1,000 
C) about $1,000,000 
D) over $400,000,000 
A) about $10 
B) about $1,000 
C) about $1,000,000 
D) over $400,000,000

Question 5

The agarose used in electrophoresis

Accepted Answer

A) interacts electrically with the DNA. 
B) chemically binds to the DNA. 
C) acts as a sieve. 
D) selectively sorts recombinant DNA from host DNA. 
A) interacts electrically with the DNA. 
B) chemically binds to the DNA. 
C) acts as a sieve. 
D) selectively sorts recombinant DNA from host DNA.

Question 6

The energy to separate fragments during agarose gel electrophoresis is supplied by

Accepted Answer

A) gravity. 
B) active transport. 
C) agarosis. 
D) electricity. 
A) gravity. 
B) active transport. 
C) agarosis. 
D) electricity.

Question 7

Digestion of DNA by restriction enzymes

Accepted Answer

A) produces sticky ends. 
B) produces blunt ends. 
C) cuts both strands of the DNA molecule. 
D) generates restriction fragments. 
E) All of the choices are correct. 
A) produces sticky ends. 
B) produces blunt ends. 
C) cuts both strands of the DNA molecule. 
D) generates restriction fragments. 
E) All of the choices are correct.

Question 8

Restriction enzymes have proved so useful in manipulating DNA because

Accepted Answer

A) they cut at defined sites. 
B) the sticky ends make it very easy to allow recombination of any type of DNA. 
C) they protect eukaryotes against virus attack. 
D) they cut RNA molecules. 
E) they cut at defined sites AND the sticky ends make it very easy to allow recombination of any type of DNA. 
A) they cut at defined sites. 
B) the sticky ends make it very easy to allow recombination of any type of DNA. 
C) they protect eukaryotes against virus attack. 
D) they cut RNA molecules. 
E) they cut at defined sites AND the sticky ends make it very easy to allow recombination of any type of DNA.

Question 9

Selecting for transformants involves

Accepted Answer

A) identifying organisms that have taken up recombinant DNA. 
B) searching for RNA. 
C) production of proteins. 
D) production of DNA. 
A) identifying organisms that have taken up recombinant DNA. 
B) searching for RNA. 
C) production of proteins. 
D) production of DNA.

Question 10

Genetic engineering

Accepted Answer

A) allows the use of bacteria as production factories for a number of molecules. 
B) relies on recombinant DNA technology. 
C) is dependent on RNA enzymes. 
D) relies completely on conjugation. 
E) allows the use of bacteria as production factories for a number of molecules AND relies on recombinant DNA technology. 
A) allows the use of bacteria as production factories for a number of molecules. 
B) relies on recombinant DNA technology. 
C) is dependent on RNA enzymes. 
D) relies completely on conjugation. 
E) allows the use of bacteria as production factories for a number of molecules AND relies on recombinant DNA technology.

Question 11

Goal(s)of gene cloning may be to produce

Accepted Answer

A) a protein. 
B) many copies of the gene to be used as a probe. 
C) many copies of the gene for sequencing. 
D) more copies of the host cell. 
E) a protein,many copies of the gene to be used as a probe,AND many copies of the gene for sequencing. 
A) a protein. 
B) many copies of the gene to be used as a probe. 
C) many copies of the gene for sequencing. 
D) more copies of the host cell. 
E) a protein,many copies of the gene to be used as a probe,AND many copies of the gene for sequencing.

Question 12

FISH uses labeled probes to detect specific whole cells.

Accepted Answer

A) True 
 B)False

Question 13

The molecules used as molecular scissors in genetic engineering are

Accepted Answer

A) exonucleases. 
B) proteases. 
C) restriction enzymes. 
D) RNA polymerases. 
A) exonucleases. 
B) proteases. 
C) restriction enzymes. 
D) RNA polymerases.

The gene for human insulin has been successfully cloned in

The size of the amplified DNA fragment generated during PCR is determined by

Host cells containing recombinant DNA can be selected on the basis of the properties of the

The current cost of sequencing a human genome is

The agarose used in electrophoresis

The energy to separate fragments during agarose gel electrophoresis is supplied by

Digestion of DNA by restriction enzymes

Restriction enzymes have proved so useful in manipulating DNA because

Selecting for transformants involves

Genetic engineering

Goal(s)of gene cloning may be to produce

FISH uses labeled probes to detect specific whole cells.

The molecules used as molecular scissors in genetic engineering are

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Dynamics of Prokaryotic Growth

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From DNA to Protein

Bacterial Genetics

Identification and Classification of Prokaryotic Organisms

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids, and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Tract Infections

Filters

Exam 9: Biotechnology and Recombinant DNA

The gene for human insulin has been successfully cloned in

The size of the amplified DNA fragment generated during PCR is determined by

Host cells containing recombinant DNA can be selected on the basis of the properties of the

The current cost of sequencing a human genome is

The agarose used in electrophoresis

The energy to separate fragments during agarose gel electrophoresis is supplied by

Digestion of DNA by restriction enzymes

Restriction enzymes have proved so useful in manipulating DNA because

Selecting for transformants involves

Genetic engineering

Goal(s)of gene cloning may be to produce

FISH uses labeled probes to detect specific whole cells.

The molecules used as molecular scissors in genetic engineering are

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Dynamics of Prokaryotic Growth

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From DNA to Protein

Bacterial Genetics

Identification and Classification of Prokaryotic Organisms

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids, and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Tract Infections

Filters