Exam 13: An Introduction to Genetic Technology

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The DNA sequencing reaction uses the enzyme DNA polymerase,a primer,and ____________________.

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D

The discovery that some bacteria ____ using enzymes that cut the viral DNA into pieces was one of the first steps in the development of recombinant DNA technology.

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During PCR,____________________ serve as a starting point for the synthesis of the new DNA strand.

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Genes isolated from mice or rats can often be used as ____________________ to identify and isolate the human version of the same gene.

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To make a typical Southern blot,genomic DNA is extracted and cut into fragments and the fragments are separated by ____________________.

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During the process of gel electrophoresis,the DNA fragments migrate due to the effects of ____.

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In recombinant DNA techniques,the gaps in the paired DNA fragments are closed by ____.

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During in vitro fertilization,the fertilized egg ____.

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Vectors can transfer ____.

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In June 2001 an announcement was made that a draft sequence of the ____________________ nucleotides in the human genome had been finished.

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A yeast artificial chromosome (YAC)is a ____ that can accommodate large DNA inserts and uses the eukaryote yeast as a host cell.

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The ability to isolate and produce many copies of specific ____________________ molecules had a great impact on many areas of genetic research.

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Nuclear transfer is a preferred method of cloning over embryo splitting because it ____.

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After a restriction enzyme cuts DNA into fragments and the fragments pair with complementary bases,the enzyme ____________________ is used to seal the gaps in the phosphate backbone.

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Growth hormones given to dairy cows would still be necessary to stimulate milk production in a cloned animal.

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The heart of a microarray is a small piece of ____________________ on which single-stranded fragments of DNA are attached at a spot called a field.

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Adult cells cannot be used as the source of nuclei for cloning animals.

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A series of techniques in which DNA fragments from an organism are linked to self-replicating vectors to create recombinant DNA molecules,which are then replicated in a host cell,is called ____________________.

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Southern blot is a method for ____.

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Detecting single nucleotide polymorphisms (SNPs),fusion genes,and mRNAs for alternative splicing are three applications of ____________________.

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