Exam 13: An Introduction to Genetic Technology

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Probes can come from a variety of sources,such as ____________________.

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An advantage of YACs over other vectors in cloning human genes is that they ____.

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Summarize the three requirements for cloning once a source of DNA has been selected.

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A collection of clones containing all the DNA sequences carried by an individual is called a genomic library.

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During the denaturation step of PCR,____ bonds are broken when DNA is heated.

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List some of the applications of DNA sequencing.

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The basic method of DNA sequencing is the Sanger method.

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Although artificial selection may seem slow and unreliable,____________________ observed that after only a few generations,breeders produced many varieties of pigeons and minks.

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DNA cloning techniques have made it possible to locate ____________________,map them,isolate them,sequence them,and transfer them between species.

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Instead of a(n)____________________ group to link nucleotides together,the modified nucleotides used during automated DNA sequencing have only an -H.

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During gel electrophoresis,DNA fragments are separated by size.

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The polymerase chain reaction allows DNA to be copied in a test tube without using a ____________________ or a host cell.

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A technique called the ____________________ has revolutionized molecular biology because it is not as labor intensive or time consuming as DNA cloning techniques.

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To interpret microarray data,it is necessary to understand the significance of the color of each dot within the array.

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Describe the process of 'reading' a microarray in order to compare the expression of genes in two different tissues--one that is normal and one that represents a disease such as cancer.

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Consumer fears and uncertainty about the safety of cloned milk and meat have prevented these products from being sold in stores even though the ____________________ has ruled that such food is safe.

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To clone an animal by embryo splitting,____.

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Describe the steps in the polymerase chain reaction (PCR).

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During DNA sequencing,a laser beam scans the fragments on the electrophoresis gel to identify the ____________________.

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Explain the role of probes in the search for a specific human gene within a genome library.

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