Question 1

A rising antibody titer a few days apart indicates

Accepted Answer

A) the patient had a past case of the disease. 
B) the patient has a current infection. 
C) nothing, people always have antibodies in their serum. 
D) All of these choices are correct. 
A) the patient had a past case of the disease. 
B) the patient has a current infection. 
C) nothing, people always have antibodies in their serum. 
D) All of these choices are correct. B

Question 2

Pulsed-field gel electrophoresis (PFGE) is often used to determine whether bacterial isolates from different cases of food-borne illness are the same and can be traced to the same source.This is an example of

Accepted Answer

A) an immunologic technique. 
B) a genetic method. 
C) a phenotypic method. 
D) a microscopic technique. 
A) an immunologic technique. 
B) a genetic method. 
C) a phenotypic method. 
D) a microscopic technique. B

Question 3

Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _______________ whereas tests that take place in a living host, such as in a patient, are described as ______________.

Accepted Answer

A) In loco; In vitro 
B) In vitro; In loco 
C) In vitro; In vivo 
D) In loco; In vivo 
A) In loco; In vitro 
B) In vitro; In loco 
C) In vitro; In vivo 
D) In loco; In vivo C

Question 4

All of the following are methods to diagnose viral infections, except

Accepted Answer

A) detection of viral nucleic acid using specific probes. 
B) Western Blot. 
C) cells taken from patient are examined for evidence of viral infection. 
D) signs and symptoms. 
E) the light microscope. 
A) detection of viral nucleic acid using specific probes. 
B) Western Blot. 
C) cells taken from patient are examined for evidence of viral infection. 
D) signs and symptoms. 
E) the light microscope.

Question 5

Some diseases are diagnosed without the need to identify microbes from a patient specimen.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following choices are true about the dyes fluorescein and rhodamine?

Accepted Answer

A) They can be used to label antibodies in immunofluorescence tests. 
B) They emit visible light in response to ultraviolet radiation. 
C) They are observed in the fluorescent microscope. 
D) They are used to identify pathogens of chlamydiosis, Legionnaires'disease, and others. 
E) All of these choices are correct. 
A) They can be used to label antibodies in immunofluorescence tests. 
B) They emit visible light in response to ultraviolet radiation. 
C) They are observed in the fluorescent microscope. 
D) They are used to identify pathogens of chlamydiosis, Legionnaires'disease, and others. 
E) All of these choices are correct.

Question 7

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Accepted Answer

The answer of In _________ tests, antibody cross-links whole-cell antigens,...

Question 8

If trying to isolate a specific pathogen from amongst a variety of normal microbiota, which type of media would be most helpful?

Accepted Answer

A) nutrient agar 
B) differential media 
C) blood agar 
D) selective and differential agar 
A) nutrient agar 
B) differential media 
C) blood agar 
D) selective and differential agar

Question 9

The indirect ELISA test detects ______ in a patient's blood.

Accepted Answer

A) antigen 
B) antibody 
C) microorganisms 
D) IgE only 
E) complement 
A) antigen 
B) antibody 
C) microorganisms 
D) IgE only 
E) complement

Question 10

Biochemical testing used for identification of pathogens involves each of the following, except

Accepted Answer

A) inoculation of additional media, incubation, and observation for a color change. 
B) quick tests that take just a few seconds. 
C) examining organisms through the microscope. 
A) inoculation of additional media, incubation, and observation for a color change. 
B) quick tests that take just a few seconds. 
C) examining organisms through the microscope.

Question 11

An antibody_____ provides a measure of the quantity of antibodies in the serum.

Accepted Answer

The answer of An antibody_____ provides a measure of the...

Question 12

Which of the following can be used for the cultivation of viruses?

Accepted Answer

A) Enriched broth media 
B) Viral host cells grown in vitro 
C) Enriched agar media 
D) All of these choices. 
A) Enriched broth media 
B) Viral host cells grown in vitro 
C) Enriched agar media 
D) All of these choices.

Question 13

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 14

Serological tests should have low sensitivity and specificity.

Accepted Answer

A) True 
 B)False

Question 15

Which technique used to diagnose a disease is also called the double diffusion method?

Accepted Answer

A) Ouchterlony test 
B) VDRL test 
C) immunoelectrophoresis 
D) Western Blot 
E) ELISA 
A) Ouchterlony test 
B) VDRL test 
C) immunoelectrophoresis 
D) Western Blot 
E) ELISA

Question 16

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

Accepted Answer

A) lower; lower 
B) higher; lower 
C) lower; higher 
D) higher; higher 
A) lower; lower 
B) higher; lower 
C) lower; higher 
D) higher; higher

Question 17

All of the following are correct about the indirect ELISA, except

Accepted Answer

A) it can detect antibodies in a serum sample. 
B) it is the common screening test for antibodies to Helicobacter. 
C) color development indicates that antibody was not present in the patient's serum. 
D) it is the common screening test for antibodies to Hepatitis A. 
E) a known antigen is adsorbed to the surface of a well. 
A) it can detect antibodies in a serum sample. 
B) it is the common screening test for antibodies to Helicobacter. 
C) color development indicates that antibody was not present in the patient's serum. 
D) it is the common screening test for antibodies to Hepatitis A. 
E) a known antigen is adsorbed to the surface of a well.

Question 18

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Accepted Answer

The answer of In a __________, antigens or antibodies are...

Question 19

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

Accepted Answer

A) immunoelectrophoresis 
B) Ouchterlony 
C) complement fixation 
D) Western Blot 
E) radioimmunoassay 
A) immunoelectrophoresis 
B) Ouchterlony 
C) complement fixation 
D) Western Blot 
E) radioimmunoassay

Question 20

When collecting __________, a "clean catch" method is often used.

Accepted Answer

The answer of When collecting __________, a "clean catch" method...

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

A rising antibody titer a few days apart indicates

Pulsed-field gel electrophoresis (PFGE) is often used to determine whether bacterial isolates from different cases of food-borne illness are the same and can be traced to the same source.This is an example of

Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _______________ whereas tests that take place in a living host, such as in a patient, are described as ______________.

All of the following are methods to diagnose viral infections, except

Some diseases are diagnosed without the need to identify microbes from a patient specimen.

Which of the following choices are true about the dyes fluorescein and rhodamine?

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

If trying to isolate a specific pathogen from amongst a variety of normal microbiota, which type of media would be most helpful?

The indirect ELISA test detects ______ in a patient's blood.

Biochemical testing used for identification of pathogens involves each of the following, except

An antibody_____ provides a measure of the quantity of antibodies in the serum.

Which of the following can be used for the cultivation of viruses?

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Serological tests should have low sensitivity and specificity.

Which technique used to diagnose a disease is also called the double diffusion method?

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

All of the following are correct about the indirect ELISA, except

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

When collecting __________, a "clean catch" method is often used.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _ whereas tests that take place in a living host, such as in a patient, are described as .

The more a sample can be diluted and yet still react with antigen, the the concentration of antibodies in that sample and the _ is its titer.