Question 1

Serotyping of the pneumococcus based on capsular polysaccharide is performed in

Accepted Answer

A) Ouchterlony double diffusion. 
B) Western Blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test. 
A) Ouchterlony double diffusion. 
B) Western Blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test.

Question 2

Broad general characteristics of bacteria on flowcharts can be misleading for a diagnosis of disease.

Accepted Answer

A) True 
 B)False

Question 3

The property of a test to detect only a certain antibody or antigen, and not to react with any others, is

Accepted Answer

A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity. 
A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity.

Question 4

In direct antigen testing, the reaction is generally seen with the

Accepted Answer

A) electron microscope. 
B) naked eye. 
C) light microscope. 
D) API 20E miniature identification system. 
E) PCR reaction. 
A) electron microscope. 
B) naked eye. 
C) light microscope. 
D) API 20E miniature identification system. 
E) PCR reaction.

Question 5

Test systems involving which of the following has greatly improved the specificity of immunological tests?

Accepted Answer

A) Monoclonal antibodies 
B) Electronics 
C) Enzymes 
D) Radioactivity 
E) All of these choices are correct. 
A) Monoclonal antibodies 
B) Electronics 
C) Enzymes 
D) Radioactivity 
E) All of these choices are correct.

Question 6

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

Accepted Answer

A) Ouchterlony double diffusion 
B) Western Blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test 
A) Ouchterlony double diffusion 
B) Western Blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test

Question 7

Minute amounts of a pathogen in a patient sample can be rapidly detected by specifically amplifying and identifying its nucleic acid using PCR.This is an example of

Accepted Answer

A) an immunologic method. 
B) a microscopic method. 
C) a phenotypic method. 
D) a genetic method. 
A) an immunologic method. 
B) a microscopic method. 
C) a phenotypic method. 
D) a genetic method.

Question 8

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Accepted Answer

A) Ouchterlony double diffusion 
B) Western Blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test 
A) Ouchterlony double diffusion 
B) Western Blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test

Question 9

Sputum is obtained by

Accepted Answer

A) swabbing the mouth to sample saliva. 
B) a clean catch urine sample. 
C) the patient coughing up mucus from the lower respiratory system. 
D) a spinal tap above the chest. 
E) a throat swab. 
A) swabbing the mouth to sample saliva. 
B) a clean catch urine sample. 
C) the patient coughing up mucus from the lower respiratory system. 
D) a spinal tap above the chest. 
E) a throat swab.

Question 10

Soluble antigens are detected in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 11

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following is involved in the in vitro diagnostic testing of serum?

Accepted Answer

A) mycology 
B) hematology 
C) serology 
D) histology 
E) virology 
A) mycology 
B) hematology 
C) serology 
D) histology 
E) virology

Question 13

Select the methods below that can be used for identifying the causative agent of a viral infection.Check all that apply.

Accepted Answer

A) cultivation of the virus from patient samples in cell/tissue cultures 
B) detection of antibodies to the virus in a patient's serum 
C) observation of the patient's symptoms 
D) gram stain of patient specimens 
E) amplification and detection of viral nucleic acid in patient specimens
F)culture of virus from patient specimens in enriched agar and broth media
G)direct observation of viral-infected cells using fluorescent-antibody staining 
A) cultivation of the virus from patient samples in cell/tissue cultures 
B) detection of antibodies to the virus in a patient's serum 
C) observation of the patient's symptoms 
D) gram stain of patient specimens 
E) amplification and detection of viral nucleic acid in patient specimens
F)culture of virus from patient specimens in enriched agar and broth media
G)direct observation of viral-infected cells using fluorescent-antibody staining

Question 14

Which test is used to measure the IgE present in allergic patients?

Accepted Answer

A) fluorescent antibody tests 
B) Rapid Plasma Reagin (RPR) test 
C) Weil-Felix reaction 
D) RIST 
E) ELISA 
A) fluorescent antibody tests 
B) Rapid Plasma Reagin (RPR) test 
C) Weil-Felix reaction 
D) RIST 
E) ELISA

Question 15

Describe three possible sampling sites and methods of collection for clinical laboratories.

Accepted Answer

1. Blood Collection from a Vein: One com

Question 16

The tuberculin test is an example of an in vivo serological test.

Accepted Answer

A) True 
 B)False

Question 17

Compare and contrast agglutination and precipitation reactions, and include a description of one test for each type of reaction.

Accepted Answer

Agglutination and precipitation reaction

Question 18

Whole antigens are detected in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 19

When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called

Accepted Answer

A) Ouchterlony double diffusion. 
B) Western Blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test. 
A) Ouchterlony double diffusion. 
B) Western Blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test.

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

Serotyping of the pneumococcus based on capsular polysaccharide is performed in

Broad general characteristics of bacteria on flowcharts can be misleading for a diagnosis of disease.

The property of a test to detect only a certain antibody or antigen, and not to react with any others, is

In direct antigen testing, the reaction is generally seen with the

Test systems involving which of the following has greatly improved the specificity of immunological tests?

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

Minute amounts of a pathogen in a patient sample can be rapidly detected by specifically amplifying and identifying its nucleic acid using PCR.This is an example of

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Sputum is obtained by

Soluble antigens are detected in which type of test?

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Which of the following is involved in the in vitro diagnostic testing of serum?

Select the methods below that can be used for identifying the causative agent of a viral infection.Check all that apply.

Which test is used to measure the IgE present in allergic patients?

Describe three possible sampling sites and methods of collection for clinical laboratories.

The tuberculin test is an example of an in vivo serological test.

Compare and contrast agglutination and precipitation reactions, and include a description of one test for each type of reaction.

Whole antigens are detected in which type of test?

When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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