Question 1

Which is incorrect about chocolate agar?

Accepted Answer

A) It can be used to cultivate Neisseria. 
B) It usually uses sheep blood that has been heated. 
C) It has chocolate extract in it. 
D) It is an enriched medium. 
E) It is used to grow fastidious bacteria. 
A) It can be used to cultivate Neisseria. 
B) It usually uses sheep blood that has been heated. 
C) It has chocolate extract in it. 
D) It is an enriched medium. 
E) It is used to grow fastidious bacteria.

Question 2

A pure culture contains only

Accepted Answer

A) one species of microorganism. 
B) bacteria. 
C) a variety of microbes from one source. 
D) All of these choices are correct. 
A) one species of microorganism. 
B) bacteria. 
C) a variety of microbes from one source. 
D) All of these choices are correct.

Question 3

Media that contains extracts from plants, animals, or yeasts are

Accepted Answer

A) synthetic. 
B) complex. 
C) reducing. 
D) enriched. 
E) All of these choices are correct. 
A) synthetic. 
B) complex. 
C) reducing. 
D) enriched. 
E) All of these choices are correct.

Question 4

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

The answer of The _____ of the microscope holds and...

Question 5

To prepare large volumes of influenza virus for use in the flu vaccines each year, the virus is grown in embryonated hen eggs.This is an example of the use of

Accepted Answer

A) enriched media. 
B) selective media. 
C) live media. 
D) complex media. 
A) enriched media. 
B) selective media. 
C) live media. 
D) complex media.

Question 6

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Accepted Answer

A) True 
 B)False

Question 7

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Accepted Answer

The answer of _____ has the same optical qualities as...

Question 8

Why is immersion oil often used when viewing specimens under the microscope?

Accepted Answer

A) to stain the cells blue 
B) to prevent the smear from drying out 
C) to slow bacterial movement so you can see cells better 
D) to increase the resolution 
E) to reduce the amount of heat reaching the slide from the light source 
A) to stain the cells blue 
B) to prevent the smear from drying out 
C) to slow bacterial movement so you can see cells better 
D) to increase the resolution 
E) to reduce the amount of heat reaching the slide from the light source

Question 9

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Accepted Answer

A) sterile swab, loop dilution, TSB 
B) hockey stick, spread plate technique, and 4 degree Celsius incubation temperature 
C) loop dilution, TSA, hockey stick, 37 degree Celsius incubator 
D) inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator 
E) sterile swab, streak plate method, 37 degree Celsius incubator 
A) sterile swab, loop dilution, TSB 
B) hockey stick, spread plate technique, and 4 degree Celsius incubation temperature 
C) loop dilution, TSA, hockey stick, 37 degree Celsius incubator 
D) inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator 
E) sterile swab, streak plate method, 37 degree Celsius incubator

Question 10

You are doing research on a bacterial species, trying to determine the nature and structure of a number of intracellular inclusions.Which type of microscopy would provide the best view of these intracellular structures?

Accepted Answer

A) scanning electron microscopy 
B) transmission electron microscopy 
C) bright-field microscopy 
D) dark-Field microscopy 
A) scanning electron microscopy 
B) transmission electron microscopy 
C) bright-field microscopy 
D) dark-Field microscopy

Question 11

Which of the following is essential for development of discrete, isolated colonies?

Accepted Answer

A) broth medium 
B) differential medium 
C) selective medium 
D) solid medium 
E) assay medium 
A) broth medium 
B) differential medium 
C) selective medium 
D) solid medium 
E) assay medium

Question 12

Which microscope is the most widely used to show stained cells against a bright background?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 13

Viruses, Rickettsia, and Chlamydia are described as obligate intracellular parasites because they can only be propagated inside living host cells.Which of the following would be appropriate for their cultivation?

Accepted Answer

A) enriched media 
B) cell or tissue cultures 
C) media that contains sheep red blood cells 
D) differential media 
A) enriched media 
B) cell or tissue cultures 
C) media that contains sheep red blood cells 
D) differential media

Question 14

Each of the following are characteristics of microorganisms that pose unique challenges to their study EXCEPT

Accepted Answer

A) the fact that they must be viewed through a microscope rather than the unaided eye. 
B) the fact that many do not have membrane-enclosed nucleus or other organelles. 
C) the fact that they often exist in complex communities and individual species may be difficult to isolate from others. 
D) the fact that they usually must be grown and studied under somewhat artificial conditions in vitro rather than in their natural habit. 
A) the fact that they must be viewed through a microscope rather than the unaided eye. 
B) the fact that many do not have membrane-enclosed nucleus or other organelles. 
C) the fact that they often exist in complex communities and individual species may be difficult to isolate from others. 
D) the fact that they usually must be grown and studied under somewhat artificial conditions in vitro rather than in their natural habit.

Question 15

Bacteria that require special growth factors and complex organic substances are called

Accepted Answer

A) fastidious. 
B) pathogenic. 
C) harmless. 
D) anaerobic. 
E) aerobic. 
A) fastidious. 
B) pathogenic. 
C) harmless. 
D) anaerobic. 
E) aerobic.

Question 16

The Six "I's" of studying microorganisms include all of the following except

Accepted Answer

A) inoculation. 
B) incubation. 
C) infection. 
D) isolation. 
E) identification. 
A) inoculation. 
B) incubation. 
C) infection. 
D) isolation. 
E) identification.

Question 17

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece 
A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece

Question 18

Identification relies entirely on biochemical test results.

Accepted Answer

A) True 
 B)False

Question 19

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A) hanging drop. 
B) fixed-stained smear. 
C) Gram stain. 
D) negative stain. 
E) flagellar stain. 
A) hanging drop. 
B) fixed-stained smear. 
C) Gram stain. 
D) negative stain. 
E) flagellar stain.

Question 20

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing 
A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

Which is incorrect about chocolate agar?

A pure culture contains only

Media that contains extracts from plants, animals, or yeasts are

The _____ of the microscope holds and allows selection of the objective lenses.

To prepare large volumes of influenza virus for use in the flu vaccines each year, the virus is grown in embryonated hen eggs.This is an example of the use of

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Why is immersion oil often used when viewing specimens under the microscope?

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

You are doing research on a bacterial species, trying to determine the nature and structure of a number of intracellular inclusions.Which type of microscopy would provide the best view of these intracellular structures?

Which of the following is essential for development of discrete, isolated colonies?

Which microscope is the most widely used to show stained cells against a bright background?

Viruses, Rickettsia, and Chlamydia are described as obligate intracellular parasites because they can only be propagated inside living host cells.Which of the following would be appropriate for their cultivation?

Each of the following are characteristics of microorganisms that pose unique challenges to their study EXCEPT

Bacteria that require special growth factors and complex organic substances are called

The Six "I's" of studying microorganisms include all of the following except

Which of the following magnifies the specimen to produce the real image of the specimen?

Identification relies entirely on biochemical test results.

The specimen preparation that is best for viewing cell motility is

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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