Question 1

Free radical damage to DNA often results in
I.pyrimidine dimers
II.point mutations
III.insertions
IV.base excision

Accepted Answer

A) I only. 
B) II only. 
C) I, II. 
D) II, III. 
E) I, IV. 
A) I only. 
B) II only. 
C) I, II. 
D) II, III. 
E) I, IV. C

Question 2

Which of the following statement(s)about DNA polymerase III (Pol III)is (are)TRUE?
I.DNA polymerase III has greater accuracy than RNA polymerase.
II.Pol III is the primary replicase of E.coli.
III.Pol III does not contain 5' to 3' exonuclease activity.

Accepted Answer

A) I, II. 
B) II, III. 
C) III only. 
D) I only. 
E) I, II, III. 
A) I, II. 
B) II, III. 
C) III only. 
D) I only. 
E) I, II, III. E

Question 3

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-The process whereby nucleotides are removed from the 5' end of one DNA segment while nucleotides are added to the 3' end of the immediately preceding DNA segment is called ______.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive H

Question 4

Use the following information to answer the next questions :
Suppose that an error in replication occurred at position 324 in the E.coli sequence below, and that a GATC motif used to regulate replication and restriction is located about 300 bp away.Note that the A on the bottom (shown by the underlining)strand is methylated.
 
-What kind of mutation has occurred?

Accepted Answer

A) transition 
B) transversion 
C) insertion 
D) deletion 
E) none of the above 
A) transition 
B) transversion 
C) insertion 
D) deletion 
E) none of the above

Question 5

Synthesis of telomeres requires the enzyme ______, which is a type of ______.

Accepted Answer

A) telomerase, helicase 
B) telomerase, ribonucleoprotein 
C) ribonuclease, riboprotein 
D) ribotelase, ligase 
E) none of the above 
A) telomerase, helicase 
B) telomerase, ribonucleoprotein 
C) ribonuclease, riboprotein 
D) ribotelase, ligase 
E) none of the above

Question 6

Defects in the proteins ______, which associate with RAD-51, are correlated with several types of cancer.

Accepted Answer

A) BRCA1 and BRCA2 
B) RecU and RecB 
C) Pol $\alpha$ and Pol $\beta$ 
D) DSB and NHEJ 
E) RecA and RecUGA 
A) BRCA1 and BRCA2 
B) RecU and RecB 
C) Pol $\alpha$ and Pol $\beta$ 
D) DSB and NHEJ 
E) RecA and RecUGA

Question 7

E.coli leading and lagging strand synthesis takes place at the

Accepted Answer

A) the Klenow fragment binding site. 
B) Pol III & Tus interface. 
C) multiprotein replisome. 
D) origin recognition complex. 
E) telomere. 
A) the Klenow fragment binding site. 
B) Pol III & Tus interface. 
C) multiprotein replisome. 
D) origin recognition complex. 
E) telomere.

Question 8

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Termination of replication fork movement in E.coli involves the binding of the ______ protein at a Ter site.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 9

Which of the following are TRUE about transposons?
I.They are normal components of plasmids.
II.They often carry genes for antibiotic resistance.
III.They insert at a target sequence that is a repetitive unit of about 200 bp.

Accepted Answer

A) I, II, III 
B) I, II 
C) II, III 
D) II only 
E) III only 
A) I, II, III 
B) I, II 
C) II, III 
D) II only 
E) III only

Question 10

A new DNA polymerase exhibits: (1)high processivity, (2)low fidelity, and (3)ability to replace RNA with DNA.Which of the following is probably TRUE?

Accepted Answer

A) It has 3' exonuclease activity. 
B) It has 5'exonuclease activity. 
C) It has RNA primase activity. 
D) It is not associated with a clamp. 
E) both A and B 
A) It has 3' exonuclease activity. 
B) It has 5'exonuclease activity. 
C) It has RNA primase activity. 
D) It is not associated with a clamp. 
E) both A and B

Question 11

Deamination of cytosine leads to the formation of

Accepted Answer

A) thymine. 
B) 5-methylcytosine. 
C) uracil. 
D) 5-bromouracil. 
E) 5-methyluracil. 
A) thymine. 
B) 5-methylcytosine. 
C) uracil. 
D) 5-bromouracil. 
E) 5-methyluracil.

Question 12

Positive correlations between mutagenesis and carcinogenesis

Accepted Answer

A) are increased with teleomere capping. 
B) are reverted via nick translation. 
C) are examined using the Ames test. 
D) increase with photoreactivation. 
E) None of the above is correct . 
A) are increased with teleomere capping. 
B) are reverted via nick translation. 
C) are examined using the Ames test. 
D) increase with photoreactivation. 
E) None of the above is correct .

Question 13

Which of the following would result in single stranded DNA?

Accepted Answer

A) Klenow fragment 
B) Klentaq I 
C) DnaB 
D) DnaG 
E) pol III 
A) Klenow fragment 
B) Klentaq I 
C) DnaB 
D) DnaG 
E) pol III

Question 14

Which of the following statements about telomerase is FALSE?
I.Telomerase is an RNA polymerase.
II.Telomerase has a 5'->3' polymerase activity.
III.Telomerase uses RNA as a template.
IV.The product of the telomerase-catalyzed reaction is rich in G and T.

Accepted Answer

A) I only 
B) II only 
C) II, III 
D) I, III 
E) II, IV 
A) I only 
B) II only 
C) II, III 
D) I, III 
E) II, IV

Question 15

Which of the following would result in increased processivity in E.coli?

Accepted Answer

A) ($\beta$ (sliding)clamp) 
B) primosome 
C) helicase 
D) Dna B 
E) Dna G 
A) ($\beta$ (sliding)clamp) 
B) primosome 
C) helicase 
D) Dna B 
E) Dna G

Question 16

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Enzymes that undergo many rounds of polymerization catalysis before dissociating from the substrate are described as highly ______.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 17

Capping is important because

Accepted Answer

A) it likely protects the telomere ends from becoming shortened. 
B) it likely prevents DNA repair mechanisms from initiating at the telomeres. 
C) it likely results in increased processivity on the eukaryotic chromosome. 
D) all of the above 
E) none of the above 
A) it likely protects the telomere ends from becoming shortened. 
B) it likely prevents DNA repair mechanisms from initiating at the telomeres. 
C) it likely results in increased processivity on the eukaryotic chromosome. 
D) all of the above 
E) none of the above

Question 18

In molecular biology labs researchers often use E.coli strains that are RecA minus (recA−)when transforming a cloned gene into a plasmid which explains why
I.a Rec A-strain would have fewer replication errors.
II.a Rec A-strain would have less opportunity forming DNA mutations.
III.a Rec A-strain would increase error repair.

Accepted Answer

A) I only. 
B) II only. 
C) III only. 
D) I, II 
E) II, III 
A) I only. 
B) II only. 
C) III only. 
D) I, II 
E) II, III

Question 19

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-The RNA segment to which DNA residues are added during replication is the ______.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 20

Pol I is proteolytically cleaved to yield the ____, which lacks 5'$\rarr$3' exonuclease activity.

Accepted Answer

A) Klenow fragment 
B) Klentaq1 polymerase 
C) Okazaki fragment 
D) ligase 
E) primase 
A) Klenow fragment 
B) Klentaq1 polymerase 
C) Okazaki fragment 
D) ligase 
E) primase

Exam 25: Dna Replication, Repair and Recombination

Free radical damage to DNA often results in I.pyrimidine dimers II.point mutations III.insertions IV.base excision

Which of the following statement(s)about DNA polymerase III (Pol III)is (are)TRUE? I.DNA polymerase III has greater accuracy than RNA polymerase. II.Pol III is the primary replicase of E.coli. III.Pol III does not contain 5' to 3' exonuclease activity.

Matching Choose the correct answer from the list. Not all the answers will be used. -The process whereby nucleotides are removed from the 5' end of one DNA segment while nucleotides are added to the 3' end of the immediately preceding DNA segment is called ______.

Synthesis of telomeres requires the enzyme ______, which is a type of ______.

Defects in the proteins ______, which associate with RAD-51, are correlated with several types of cancer.

E.coli leading and lagging strand synthesis takes place at the

Matching Choose the correct answer from the list. Not all the answers will be used. -Termination of replication fork movement in E.coli involves the binding of the ______ protein at a Ter site.

Which of the following are TRUE about transposons? I.They are normal components of plasmids. II.They often carry genes for antibiotic resistance. III.They insert at a target sequence that is a repetitive unit of about 200 bp.

A new DNA polymerase exhibits: (1)high processivity, (2)low fidelity, and (3)ability to replace RNA with DNA.Which of the following is probably TRUE?

Deamination of cytosine leads to the formation of

Positive correlations between mutagenesis and carcinogenesis

Which of the following would result in single stranded DNA?

Which of the following statements about telomerase is FALSE? I.Telomerase is an RNA polymerase. II.Telomerase has a 5'->3' polymerase activity. III.Telomerase uses RNA as a template. IV.The product of the telomerase-catalyzed reaction is rich in G and T.

Which of the following would result in increased processivity in E.coli?

Matching Choose the correct answer from the list. Not all the answers will be used. -Enzymes that undergo many rounds of polymerization catalysis before dissociating from the substrate are described as highly ______.

Capping is important because

Matching Choose the correct answer from the list. Not all the answers will be used. -The RNA segment to which DNA residues are added during replication is the ______.

Pol I is proteolytically cleaved to yield the ____, which lacks 5' →\rarr→ 3' exonuclease activity.

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Transcription and Rna Processing

Translation

Regulation of Gene Expression

Filters

Synthesis of telomeres requires the enzyme , which is a type of .

Pol I is proteolytically cleaved to yield the ____, which lacks 5' $\rarr$ 3' exonuclease activity.