Question 1

Which of the following enzymes catalyze the creation of AP sites for base excision repair?

Accepted Answer

A) exonuclease 
B) endonuclease 
C) ligase 
D) glycosylase 
E) methylase 
A) exonuclease 
B) endonuclease 
C) ligase 
D) glycosylase 
E) methylase

Question 2

In most organisms, replication proceeds in a ______ manner from the ______.

Accepted Answer

A) discontinuous; leading strand 
B) unidirectional; chromosome ends 
C) bidirectional; replication origin 
D) bidirectional; chromosome ends 
E) discontinuous; chromosome ends 
A) discontinuous; leading strand 
B) unidirectional; chromosome ends 
C) bidirectional; replication origin 
D) bidirectional; chromosome ends 
E) discontinuous; chromosome ends

Question 3

Which of the following describes the Holliday junction?

Accepted Answer

A) point where DNA base excision repair initiates 
B) crossover point involving 4-stranded DNA structure in homologous recombination 
C) insertion point of a gene flanked in transposons 
D) point where MutS binds to initiate SOS response 
E) none of the above 
A) point where DNA base excision repair initiates 
B) crossover point involving 4-stranded DNA structure in homologous recombination 
C) insertion point of a gene flanked in transposons 
D) point where MutS binds to initiate SOS response 
E) none of the above

Question 4

Which of the following activities does E.coli DNA polymerase III contain?
I.5'→3' polymerase
II.5'→3' exonuclease
III.3'→5' exonuclease

Accepted Answer

A) I only 
B) I, II 
C) II only 
D) I, III 
E) I, II, III 
A) I only 
B) I, II 
C) II only 
D) I, III 
E) I, II, III

Question 5

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-In yeast, DNA replication begins at sequences known as ______.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 6

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-E.coli primer synthesis is catalyzed by a ______.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 7

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Genes can be moved by _______.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 8

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-DNA separated at the replication fork is stabilized by ______ .

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 9

Which of the following statements about DNA methylation is TRUE?
I.Methylation plays a role in genomic imprinting.
II.Methylation can influence gene regulation.
III.Methylation plays a role in restriction modification systems.
IV.Methylation occurs in prokaryotic cells, but not eukaryotic cells.

Accepted Answer

A) I, II, III, IV 
B) I, II, III 
C) II, III 
D) II, III, IV 
E) II only 
A) I, II, III, IV 
B) I, II, III 
C) II, III 
D) II, III, IV 
E) II only

Question 10

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Direct reversal of pyrimidine dimers in prokaryotic cells is catalyzed by a DNA _____.

Accepted Answer

A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive 
A) SSB 
B) Tus 
C) primosome 
D) ARS 
E) ATS
F)primer
G)transposons
H)nick translation
I)photolyase
J)processive
K)G-loops
L)G-quartets
M)accurate
N)lagging strand synthesis
O)MCM
P)abortive

Question 11

Which of the following statements about Pol I is FALSE?

Accepted Answer

A) Experiments demonstrate that Pol I exhibits exonuclease activity. 
B) Pol I is not the primary replicase in E.coli. 
C) Mutation analysis demonstrates that Pol I functions in DNA repair. 
D) Mutation analysis demonstrates the Pol I activity is not essential for cell viability. 
E) Experiments have successfully utilized Pol I to radioactively label DNA in lab experiments. 
A) Experiments demonstrate that Pol I exhibits exonuclease activity. 
B) Pol I is not the primary replicase in E.coli. 
C) Mutation analysis demonstrates that Pol I functions in DNA repair. 
D) Mutation analysis demonstrates the Pol I activity is not essential for cell viability. 
E) Experiments have successfully utilized Pol I to radioactively label DNA in lab experiments.

Question 12

Pol I synthesizes new DNA with very high fidelity, due to its

Accepted Answer

A) high processivity. 
B) 3'  $\rarr$5' exonuclease activity.  
C) helicase association with the primase. 
D) 5'  $\rarr$ 3'exonuclease activity. 
E) all of the above. 
A) high processivity. 
B) 3'  $\rarr$5' exonuclease activity.  
C) helicase association with the primase. 
D) 5'  $\rarr$ 3'exonuclease activity. 
E) all of the above.

Question 13

Considering the following eukaryotic DNA polymerases, which of the following has the highest fidelity?

Accepted Answer

A) DNA pol $\alpha$ 
B) DNA pol $\beta$ 
C) DNA pol $\delta$ 
D) All have high fidelity 
E) All have low fidelity 
A) DNA pol $\alpha$ 
B) DNA pol $\beta$ 
C) DNA pol $\delta$ 
D) All have high fidelity 
E) All have low fidelity

Question 14

Which of the following can be extended by DNA polymerase?

Accepted Answer

A) a single strand of DNA ending in a nucleotide with a free 3' OH 
B) a RNA single strand with a ribonucleotide with a free 3' OH 
C) a single strand DNA end in a deoxyribonucleotide with free 5' P 
D) a RNA single strand 
E) all of the above 
A) a single strand of DNA ending in a nucleotide with a free 3' OH 
B) a RNA single strand with a ribonucleotide with a free 3' OH 
C) a single strand DNA end in a deoxyribonucleotide with free 5' P 
D) a RNA single strand 
E) all of the above

Question 15

DNA ligase can be activated by which of the following?
I.The coupled hydrolysis of NAD+ to (NMN+)+ AMP.
II.The coupled hydrolysis of ATP to AMP + PPi.
III.The coupled hydrolysis of GTP to GMP + PPi.

Accepted Answer

A) I only 
B) II only 
C) III only 
D) I, II 
E) I, III 
A) I only 
B) II only 
C) III only 
D) I, II 
E) I, III

Question 16

Replication of circular DNA occurs at the "bubbles" or "eyes", also called ______ .

Accepted Answer

A) ($\theta$ structures) 
B) ($\Phi$ bubbles) 
C) ($\theta$ bubbles) 
D) ( $\sigma$ structures) 
E) ( $\sigma$ bubbles) 
A) ($\theta$ structures) 
B) ($\Phi$ bubbles) 
C) ($\theta$ bubbles) 
D) ( $\sigma$ structures) 
E) ( $\sigma$ bubbles)

Question 17

The presence of proflavin is likely to induce

Accepted Answer

A) pyrimidine dimers. 
B) point mutations. 
C) deletions. 
D) transversions. 
E) Any of the above may occur. 
A) pyrimidine dimers. 
B) point mutations. 
C) deletions. 
D) transversions. 
E) Any of the above may occur.

Question 18

The experiments of Reiji Okazaki allowed determination that DNA synthesis occurs in
I.a continuous fashion.
II.different ways on the lagging strand than the leading strand.
III.resulted in new synthesized DNA fragments containing the radioactive isotope.

Accepted Answer

A) I, II, III 
B) II, III 
C) I, II 
D) I, III 
E) II only 
A) I, II, III 
B) II, III 
C) I, II 
D) I, III 
E) II only

Question 19

Bacteria have obtained the $\beta$-lactamase gene which encodes a protein that renders all $\beta$-lactem antibodies such as ampicillin and penicillin inactive.Which of the following would result in an increased number of bacteria with this ability?
I.a transposon with the $\beta$-lactamase gene
II.a recombination event within the original strain that results in a point mutation inactivating $\beta$-lactamase
III.an insertion in the original strain rendering transposase inactive
IV.a deletion in the insertion sequence of the transposon

Accepted Answer

A) I, II, III, IV 
B) I only 
C) II only 
D) II, III 
E) III, IV 
A) I, II, III, IV 
B) I only 
C) II only 
D) II, III 
E) III, IV

Question 20

The DNA sequence shown below is copied by the Klenow fragment.Which of the following would most likely result? 5' TGCGTGCACTCGA 3'

Accepted Answer

A) identical 
B) 5' TGCGT 3' 
C) 5' TGCAGTGACGCT 3' 
D) cannot predict 
E) none of the above 
A) identical 
B) 5' TGCGT 3' 
C) 5' TGCAGTGACGCT 3' 
D) cannot predict 
E) none of the above

Exam 25: Dna Replication, Repair and Recombination

Which of the following enzymes catalyze the creation of AP sites for base excision repair?

In most organisms, replication proceeds in a ______ manner from the ______.

Which of the following describes the Holliday junction?

Which of the following activities does E.coli DNA polymerase III contain? I.5'→3' polymerase II.5'→3' exonuclease III.3'→5' exonuclease

Matching Choose the correct answer from the list. Not all the answers will be used. -In yeast, DNA replication begins at sequences known as ______.

Matching Choose the correct answer from the list. Not all the answers will be used. -E.coli primer synthesis is catalyzed by a ______.

Matching Choose the correct answer from the list. Not all the answers will be used. -Genes can be moved by _______.

Matching Choose the correct answer from the list. Not all the answers will be used. -DNA separated at the replication fork is stabilized by ______ .

Which of the following statements about DNA methylation is TRUE? I.Methylation plays a role in genomic imprinting. II.Methylation can influence gene regulation. III.Methylation plays a role in restriction modification systems. IV.Methylation occurs in prokaryotic cells, but not eukaryotic cells.

Matching Choose the correct answer from the list. Not all the answers will be used. -Direct reversal of pyrimidine dimers in prokaryotic cells is catalyzed by a DNA _____.

Which of the following statements about Pol I is FALSE?

Pol I synthesizes new DNA with very high fidelity, due to its

Considering the following eukaryotic DNA polymerases, which of the following has the highest fidelity?

Which of the following can be extended by DNA polymerase?

DNA ligase can be activated by which of the following? I.The coupled hydrolysis of NAD+ to (NMN+)+ AMP. II.The coupled hydrolysis of ATP to AMP + PPi. III.The coupled hydrolysis of GTP to GMP + PPi.

Replication of circular DNA occurs at the "bubbles" or "eyes", also called ______ .

The presence of proflavin is likely to induce

The experiments of Reiji Okazaki allowed determination that DNA synthesis occurs in I.a continuous fashion. II.different ways on the lagging strand than the leading strand. III.resulted in new synthesized DNA fragments containing the radioactive isotope.

The DNA sequence shown below is copied by the Klenow fragment.Which of the following would most likely result? 5' TGCGTGCACTCGA 3'

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Transcription and Rna Processing

Translation

Regulation of Gene Expression

Filters

In most organisms, replication proceeds in a manner from the .

DNA ligase can be activated by which of the following? I.The coupled hydrolysis of NAD⁺ to (NMN⁺)+ AMP. II.The coupled hydrolysis of ATP to AMP + PP_i. III.The coupled hydrolysis of GTP to GMP + PP_i.