Question 1

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Protein-coding genes are also known as ______ genes.

Accepted Answer

A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome 
A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome F

Question 2

What function is accomplished by snoRNAs?

Accepted Answer

A) spicing site recognition 
B) capping of the mRNA 
C) methylation of tRNAs 
D) methylation of rRNA 
E) splicosome catalytic site recognition 
A) spicing site recognition 
B) capping of the mRNA 
C) methylation of tRNAs 
D) methylation of rRNA 
E) splicosome catalytic site recognition D

Question 3

Why is TBP referred to as a universal transcription factor?
I.TBP is present in both prokaryotic and eukaryotic organisms.
II.TBP catalyzes the initiation of all RNA polymersase in both prokaryotes and eukaryotes.
III.TBP is required for initiation by RNAP I, RNAP II, and RNAP III.

Accepted Answer

A) I, II, III 
B) I, II 
C) I, III 
D) II, III 
E) III only 
A) I, II, III 
B) I, II 
C) I, III 
D) II, III 
E) III only C

Question 4

The processing of pre-rRNAs includes ______.
I.nucleolytic cleavage.
II.snoRNA-assisted methylation.
III.snRNP-assisted methylation.

Accepted Answer

A) I, II 
B) I, III 
C) II, III 
D) I, II, III 
E) III only 
A) I, II 
B) I, III 
C) II, III 
D) I, II, III 
E) III only

Question 5

In prokaryotes, the _____ factor recognizes base sequences in the _____ which facilitates RNA polymerase binding and initiates RNA synthesis.

Accepted Answer

A) (F1F1F1S1F1F1F10; promoter) 
B) regulatory; enhancer 
C) (F1F1F1S1F1F1F10; enhancer) 
D) regulatory; promoter 
E) omega; promoter 
A) (F1F1F1S1F1F1F10; promoter) 
B) regulatory; enhancer 
C) (F1F1F1S1F1F1F10; enhancer) 
D) regulatory; promoter 
E) omega; promoter

Question 6

Which of the following is FALSE regarding prokaryotic transcription termination?

Accepted Answer

A) It can occur in a factor dependent fashion. 
B) It can occur in a factor independent fashion. 
C) It may involve the Rho protein. 
D) all of the above 
E) none of the above 
A) It can occur in a factor dependent fashion. 
B) It can occur in a factor independent fashion. 
C) It may involve the Rho protein. 
D) all of the above 
E) none of the above

Question 7

The term rRNA refers to ______ RNA.

Accepted Answer

A) ribosomal 
B) retroviral 
C) recombinant 
D) rho factor 
A) ribosomal 
B) retroviral 
C) recombinant 
D) rho factor

Question 8

The phosphorylation of the CTD of RNA polymerase II is important for which phase of transcription?

Accepted Answer

A) initial binding of the promoter 
B) conversion from the pre-initiation (closed)complex to the initiation (open)complex 
C) progression from initiation to elongation 
D) termination of transcription 
E) none of the above 
A) initial binding of the promoter 
B) conversion from the pre-initiation (closed)complex to the initiation (open)complex 
C) progression from initiation to elongation 
D) termination of transcription 
E) none of the above

Question 9

Most ______ have well defined 3F1F1F1S1F1F1F10 ends terminating in poly(A)tails of ~ 250 nt.

Accepted Answer

A) prokaryotic mRNAs 
B) eukaryotic mRNAs 
C) prokaryotic rRNAs 
D) eukaryotic rRNAs 
E) all of the above 
A) prokaryotic mRNAs 
B) eukaryotic mRNAs 
C) prokaryotic rRNAs 
D) eukaryotic rRNAs 
E) all of the above

Question 10

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-The expressed sequences of eukaryotic pre-mRNAs are known as ______.

Accepted Answer

A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome 
A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome

Question 11

The function of the TATA box is

Accepted Answer

A) to recognize the F1F1F1S1F1F1F10 subunit and correctly line up the RNA polymerase. 
B) to terminate active RNA transcription. 
C) to form a hairpin for RNA transcription termination. 
D) to provide a binding location for the Rho protein. 
E) to stimulate formation of the transcription bubble. 
A) to recognize the F1F1F1S1F1F1F10 subunit and correctly line up the RNA polymerase. 
B) to terminate active RNA transcription. 
C) to form a hairpin for RNA transcription termination. 
D) to provide a binding location for the Rho protein. 
E) to stimulate formation of the transcription bubble.

Question 12

In eukaryotes, which of the following would decrease the rate of transcription?

Accepted Answer

A) mutation in the nucleotide sequences at −10 and −35 
B) mutation in the nucleotide sequences at −10 and +5 
C) mutation in the nucleotide sequences at −70 and −90 
D) mutation at the F1F1F1S1F1F1F10 binding site 
E) all of the above 
A) mutation in the nucleotide sequences at −10 and −35 
B) mutation in the nucleotide sequences at −10 and +5 
C) mutation in the nucleotide sequences at −70 and −90 
D) mutation at the F1F1F1S1F1F1F10 binding site 
E) all of the above

Question 13

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Prokaryotic contain nucleotide sequences know as _____ which contain related genes with related functions.

Accepted Answer

A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome 
A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome

Question 14

Which of the following eukaryotic proteins are encoded by genes which have no known introns?

Accepted Answer

A) interferons 
B) tropomyosin 
C) SXL protein 
D) all of the above 
E) none of the above 
A) interferons 
B) tropomyosin 
C) SXL protein 
D) all of the above 
E) none of the above

Question 15

Which of the following best describes the function of poly(A)tails for eukaryotic mRNAs?

Accepted Answer

A) They are required for accurate translation. 
B) They have a protective role against degradation. 
C) They signal transcription termination. 
D) They function as splice sites. 
E) No role has been observed for these features. 
A) They are required for accurate translation. 
B) They have a protective role against degradation. 
C) They signal transcription termination. 
D) They function as splice sites. 
E) No role has been observed for these features.

Question 16

Splicing
I.occurs in the cytosol.
II.results from 2' -OH on the exon attack on the 5' splice site.
III.is completed when the 3' -OH on the newly cut exon attacks the 5'-terminal phosphate on the next exon.

Accepted Answer

A) I, II 
B) I, III 
C) I only 
D) II only 
E) III only 
A) I, II 
B) I, III 
C) I only 
D) II only 
E) III only

Question 17

Which of the following best describes the cap nucleotide on a eukaryotic mRNA?

Accepted Answer

A) A 
B) C 
C) m7G 
D) T 
E) U 
A) A 
B) C 
C) m7G 
D) T 
E) U

Question 18

The precursors of eukaryotic mRNAs are synthesized by ______.
I.RNA polymerase I.
II.RNA polymerase II.
III.RNA polymerase III.

Accepted Answer

A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only 
A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only

Question 19

Imagine that a new drug is proposed which is hypothesized to selectively bind the transcription factor TBP.Which of the following would be affected?
I.RNA polymerase I
II.RNA polymerase II
III.RNA polymerase III
IV.E.coli RNA polymerase

Accepted Answer

A) (F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10V) 
B) I, II, III 
C) I only 
D) II only 
E) IV only 
A) (F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10F1F1F1S1F1F1F10V) 
B) I, II, III 
C) I only 
D) II only 
E) IV only

Question 20

Which of the following types of RNA undergo posttranscriptional modifications?

Accepted Answer

A) mRNA 
B) rRNA 
C) tRNA 
D) all of the above 
E) none of the above 
A) mRNA 
B) rRNA 
C) tRNA 
D) all of the above 
E) none of the above

Exam 26: Transcription and Rna Processing

Matching Choose the correct answer from the list. Not all the answers will be used. -Protein-coding genes are also known as ______ genes.

What function is accomplished by snoRNAs?

Why is TBP referred to as a universal transcription factor? I.TBP is present in both prokaryotic and eukaryotic organisms. II.TBP catalyzes the initiation of all RNA polymersase in both prokaryotes and eukaryotes. III.TBP is required for initiation by RNAP I, RNAP II, and RNAP III.

The processing of pre-rRNAs includes ______. I.nucleolytic cleavage. II.snoRNA-assisted methylation. III.snRNP-assisted methylation.

In prokaryotes, the _____ factor recognizes base sequences in the _____ which facilitates RNA polymerase binding and initiates RNA synthesis.

Which of the following is FALSE regarding prokaryotic transcription termination?

The term rRNA refers to ______ RNA.

The phosphorylation of the CTD of RNA polymerase II is important for which phase of transcription?

Most ______ have well defined 3 ends terminating in poly(A)tails of ~ 250 nt.

Matching Choose the correct answer from the list. Not all the answers will be used. -The expressed sequences of eukaryotic pre-mRNAs are known as ______.

The function of the TATA box is

In eukaryotes, which of the following would decrease the rate of transcription?

Matching Choose the correct answer from the list. Not all the answers will be used. -Prokaryotic contain nucleotide sequences know as _____ which contain related genes with related functions.

Which of the following eukaryotic proteins are encoded by genes which have no known introns?

Which of the following best describes the function of poly(A)tails for eukaryotic mRNAs?

Splicing I.occurs in the cytosol. II.results from 2' -OH on the exon attack on the 5' splice site. III.is completed when the 3' -OH on the newly cut exon attacks the 5'-terminal phosphate on the next exon.

Which of the following best describes the cap nucleotide on a eukaryotic mRNA?

The precursors of eukaryotic mRNAs are synthesized by ______. I.RNA polymerase I. II.RNA polymerase II. III.RNA polymerase III.

Imagine that a new drug is proposed which is hypothesized to selectively bind the transcription factor TBP.Which of the following would be affected? I.RNA polymerase I II.RNA polymerase II III.RNA polymerase III IV.E.coli RNA polymerase

Which of the following types of RNA undergo posttranscriptional modifications?

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Translation

Regulation of Gene Expression

Filters

In prokaryotes, the _ factor recognizes base sequences in the _ which facilitates RNA polymerase binding and initiates RNA synthesis.