Question 1

In which of the following types of eukaryotics RNAs are introns completely removed most of the time, although exceptions can occur?
I.mRNA
II.rRNA
III.tRNA

Accepted Answer

A) I, II 
B) I, III 
C) II, III 
D) I, II, III 
E) none of the above 
A) I, II 
B) I, III 
C) II, III 
D) I, II, III 
E) none of the above

Question 2

Which of the following are components of the spliceosome?
I.primary transcript RNA.
II.snRNPs.
III.primary transcript RNA binding proteins.

Accepted Answer

A) I, II 
B) I, III 
C) II, III 
D) I, II, III 
E) III only 
A) I, II 
B) I, III 
C) II, III 
D) I, II, III 
E) III only

Question 3

The precursors of 5S rRNA, the tRNAs, and a variety of other small nuclear and cytosolic RNAs are synthesized by ______.
I.RNA polymerase I.
II.RNA polymerase II.
III.RNA polymerase III.

Accepted Answer

A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only 
A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only

Question 4

All cellular RNAs are transcribed from

Accepted Answer

A) DNA templates. 
B) RNA templates. 
C) either DNA or RNA templates, but not both in the same organism. 
D) a combination of DNA and RNA templates. 
E) none of the above 
A) DNA templates. 
B) RNA templates. 
C) either DNA or RNA templates, but not both in the same organism. 
D) a combination of DNA and RNA templates. 
E) none of the above

Question 5

Which of the following is correct regarding splicing?

Accepted Answer

A) CPSFs are products of RNA sequences designed to recognize the 3'OH at the splice site. 
B) PABIII recognizes the A rich sequences at the 5' end of the splice site. 
C) Lariats are the sequence recognized by the cleavage site. 
D) smURFs are used to recognize the poly A tail and initiate slicing 
E) snRNPs are the protein product of U rich RNAs that partially complement the 5' splice junction. 
A) CPSFs are products of RNA sequences designed to recognize the 3'OH at the splice site. 
B) PABIII recognizes the A rich sequences at the 5' end of the splice site. 
C) Lariats are the sequence recognized by the cleavage site. 
D) smURFs are used to recognize the poly A tail and initiate slicing 
E) snRNPs are the protein product of U rich RNAs that partially complement the 5' splice junction.

Question 6

Which of the following statements best describes the role of F1F1F1S1F1F1F10 factors in bacterial gene expression?

Accepted Answer

A) (F1F1F1S1F1F1F10 factors determine which RNA polymerase is used during transcription. 
B) (F1F1F1S1F1F1F10 factors mediate the excision of intronic sequences. 
C) (F1F1F1S1F1F1F10 factors can be used to express certain genes necessary based on cell type. 
D) (F1F1F1S1F1F1F10 factors bind to the TATA box and initiate transcription. 
E) none of the above 
A) (F1F1F1S1F1F1F10 factors determine which RNA polymerase is used during transcription. 
B) (F1F1F1S1F1F1F10 factors mediate the excision of intronic sequences. 
C) (F1F1F1S1F1F1F10 factors can be used to express certain genes necessary based on cell type. 
D) (F1F1F1S1F1F1F10 factors bind to the TATA box and initiate transcription. 
E) none of the above

Question 7

Which of the following increases the stability of the elongation complex during transcription in E.coli?

Accepted Answer

A) formation of the open complex 
B) dissociation of the F1F1F1S1F1F1F10 subunit 
C) mutations in the promoter region 
D) production of abortive transcripts 
E) All of the above increase the stability. 
A) formation of the open complex 
B) dissociation of the F1F1F1S1F1F1F10 subunit 
C) mutations in the promoter region 
D) production of abortive transcripts 
E) All of the above increase the stability.

Question 8

How does the preinitiation complex begin to form at a TATA box-containing promoter?

Accepted Answer

A) RNAP binds to the sigma factor. 
B) RNAP binds to the initiator (Inr)element. 
C) TATA-binding protein binds to the TATA box. 
D) RNAP binds to the Rho factor. 
E) none of the above. 
A) RNAP binds to the sigma factor. 
B) RNAP binds to the initiator (Inr)element. 
C) TATA-binding protein binds to the TATA box. 
D) RNAP binds to the Rho factor. 
E) none of the above.

Question 9

Which is TRUE regarding structure and function of RNAPII?
I.Distortion of the DNA by ~90° isolates the template base such that it can be properly paired.
II.The "clamp" moves over the DNA increasing processivity.
III.RNAPII accomplishes proofreading by forcing mispaired nucleotides against the
"wall" causing distortion such that mispairs can be cleaved by the "rudder."

Accepted Answer

A) I, II, III 
B) I, II 
C) II, III 
D) II only 
E) III only 
A) I, II, III 
B) I, II 
C) II, III 
D) II only 
E) III only

Question 10

In eukaryotes, mRNAs are synthesized in the cell ______, whereas most translation occurs in the ______.

Accepted Answer

A) nucleus, nucleosome. 
B) nucleus, cytosol. 
C) cytosol, nucleosome. 
D) cytosol, nucleus. 
E) nucleosome, cytosol. 
A) nucleus, nucleosome. 
B) nucleus, cytosol. 
C) cytosol, nucleosome. 
D) cytosol, nucleus. 
E) nucleosome, cytosol.

Question 11

The fact that some eukaryotic rRNAs are self-splicing indicates that

Accepted Answer

A) RNA structures are highly variable. 
B) RNAs can contain modified bases. 
C) eukaryotes have multiple RNA polymerases. 
D) RNA can act as an enzyme or catalyst. 
E) None of the above is correct. 
A) RNA structures are highly variable. 
B) RNAs can contain modified bases. 
C) eukaryotes have multiple RNA polymerases. 
D) RNA can act as an enzyme or catalyst. 
E) None of the above is correct.

Question 12

In eukaryotes all three RNA polymerases recognize

Accepted Answer

A) the promoter sequence bound by F1F1F1S1F1F1F10 factor. 
B) a single promoter sequence named TF II D. 
C) class I promoters. 
D) promoters with TATA boxes. 
E) none of the above. 
A) the promoter sequence bound by F1F1F1S1F1F1F10 factor. 
B) a single promoter sequence named TF II D. 
C) class I promoters. 
D) promoters with TATA boxes. 
E) none of the above.

Question 13

The precursors of most eukaryotic rRNAs are synthesized by
I.RNA polymerase I.
II.RNA polymerase II.
III.RNA polymerase III.

Accepted Answer

A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only 
A) I or III 
B) I or II 
C) I only 
D) II only 
E) III only

Question 14

Which of the following would result in the tightest binding association between E.coli RNA polymerase and DNA?

Accepted Answer

A) core enzyme binding single stranded DNA at the promoter region 
B) open complex bound to the Pribnow box 
C) core enzyme binding duplex DNA following promoter recognition 
D) haloenzyme binding duplex DNA at the promoter site 
E) haloenzyme binding duplex DNA following promoter recognition 
A) core enzyme binding single stranded DNA at the promoter region 
B) open complex bound to the Pribnow box 
C) core enzyme binding duplex DNA following promoter recognition 
D) haloenzyme binding duplex DNA at the promoter site 
E) haloenzyme binding duplex DNA following promoter recognition

Question 15

Which of the following DNA sequences would most likely result in transcription termination?

Accepted Answer

A) 5' GCGCCTAGGCGCATTTTT 3' 3' CGCGGATCCGCGTAAAAA 5
' 
B) 5' GCGCCCGCTTTTTT 3' 3' CGCGGGCCAAAAAA 5' 
C) 5' GCGCCCGCAAAAAA 3' 3' CGCGGGCCTTTTTT 5' 
D) 5' GCGCCTAGGCGCAAAAAAA 3' 3' CGCGGATCCGCGTTTTTTT 5' 
E) none of the above 
A) 5' GCGCCTAGGCGCATTTTT 3' 3' CGCGGATCCGCGTAAAAA 5
' 
B) 5' GCGCCCGCTTTTTT 3' 3' CGCGGGCCAAAAAA 5' 
C) 5' GCGCCCGCAAAAAA 3' 3' CGCGGGCCTTTTTT 5' 
D) 5' GCGCCTAGGCGCAAAAAAA 3' 3' CGCGGATCCGCGTTTTTTT 5' 
E) none of the above

Question 16

Which of the following describes the cap nucleotide linkage on a eukaryotic mRNA?
I.5'→5' linkage
II.5'→3' linkage
III.3'→5' linkage
IV.3'→3' linkage

Accepted Answer

A) I or II 
B) I or III 
C) I only 
D) II only 
E) II or IV 
A) I or II 
B) I or III 
C) I only 
D) II only 
E) II or IV

Question 17

What is abortive initiation of transcription by RNA polymerase?

Accepted Answer

A) initiation at nonpromoter sites prior to dissociation of the polymerase. 
B) synthesis of short transcripts about 10 nucleotides long prior to commitment to elongation. 
C) pausing by the polymerase during elongation. 
D) failure to undergo the closed to open transition in the pre-initiation complex. 
E) none of the above 
A) initiation at nonpromoter sites prior to dissociation of the polymerase. 
B) synthesis of short transcripts about 10 nucleotides long prior to commitment to elongation. 
C) pausing by the polymerase during elongation. 
D) failure to undergo the closed to open transition in the pre-initiation complex. 
E) none of the above

Question 18

Transcriptional activators that can have variable positions and orientations are called

Accepted Answer

A) core promoter elements. 
B) upstream promoter elements. 
C) CpG islands. 
D) enhancers. 
E) Inr elements. 
A) core promoter elements. 
B) upstream promoter elements. 
C) CpG islands. 
D) enhancers. 
E) Inr elements.

Question 19

Which of the following is required for correct translation in eukaryote initiation?

Accepted Answer

A) poly(
A) tail. 
B) 5' cap consisting of 7-methylguanosine. 
C) intron excision. 
D) alternative splicing. 
E) exon skipping. 
A) poly(
A) tail. 
B) 5' cap consisting of 7-methylguanosine. 
C) intron excision. 
D) alternative splicing. 
E) exon skipping.

Question 20

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-Termination of bacterial transcription requires the ______ factor.

Accepted Answer

A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome 
A) Rho (Þ) 
B) introns 
C) sigma (σ) 
D) sense 
E) operons
F)structural
G)Pribnow
H)promoters
I)antisense
J)exons
K)nucleolus
L)TATA
M)Sense
N)AAAA (poly 
A) 
O)Ribosome

Exam 26: Transcription and Rna Processing

In which of the following types of eukaryotics RNAs are introns completely removed most of the time, although exceptions can occur? I.mRNA II.rRNA III.tRNA

Which of the following are components of the spliceosome? I.primary transcript RNA. II.snRNPs. III.primary transcript RNA binding proteins.

The precursors of 5S rRNA, the tRNAs, and a variety of other small nuclear and cytosolic RNAs are synthesized by ______. I.RNA polymerase I. II.RNA polymerase II. III.RNA polymerase III.

All cellular RNAs are transcribed from

Which of the following is correct regarding splicing?

Which of the following statements best describes the role of  factors in bacterial gene expression?

Which of the following increases the stability of the elongation complex during transcription in E.coli?

How does the preinitiation complex begin to form at a TATA box-containing promoter?

In eukaryotes, mRNAs are synthesized in the cell ______, whereas most translation occurs in the ______.

The fact that some eukaryotic rRNAs are self-splicing indicates that

In eukaryotes all three RNA polymerases recognize

The precursors of most eukaryotic rRNAs are synthesized by I.RNA polymerase I. II.RNA polymerase II. III.RNA polymerase III.

Which of the following would result in the tightest binding association between E.coli RNA polymerase and DNA?

Which of the following DNA sequences would most likely result in transcription termination?

Which of the following describes the cap nucleotide linkage on a eukaryotic mRNA? I.5'→5' linkage II.5'→3' linkage III.3'→5' linkage IV.3'→3' linkage

What is abortive initiation of transcription by RNA polymerase?

Transcriptional activators that can have variable positions and orientations are called

Which of the following is required for correct translation in eukaryote initiation?

Matching Choose the correct answer from the list. Not all the answers will be used. -Termination of bacterial transcription requires the ______ factor.

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Translation

Regulation of Gene Expression

Filters

In eukaryotes, mRNAs are synthesized in the cell , whereas most translation occurs in the .