Question 1

Since there are 20 standard amino acids,the number of possible linear polypeptides of length N can be expressed as:

Accepted Answer

A) n × 20 
B) 20n 
C) 20 × 10n 
D) n20 
E) n × 1020 
A) n × 20 
B) 20n 
C) 20 × 10n 
D) n20 
E) n × 1020

Question 2

A fast way for nature to generate new proteins is:

Accepted Answer

A) generation of pseudogenes. 
B) mutation by neutral drift. 
C) shuffling protein domains or motifs. 
D) hypervariable positions. 
E) liberal substitution. 
A) generation of pseudogenes. 
B) mutation by neutral drift. 
C) shuffling protein domains or motifs. 
D) hypervariable positions. 
E) liberal substitution.

Question 3

The pK1,pK2,and pKR of the amino acid lysine are 2.2,9.1,and 10.5,respectively.The pK1,pK2,and pKR of the amino acid arginine are 1.8,9.0,and 12.5,respectively.A student at SDSU wants to use ion exchange chromatography to separate lysine from arginine.What pH is likely to work best for this separation?

Accepted Answer

A) 1)5 
B) 2)5 
C) 5)5 
D) 7)5 
E) 10.5 
A) 1)5 
B) 2)5 
C) 5)5 
D) 7)5 
E) 10.5

Question 4

Which of the following has the most dramatic influence on the characteristics of an individual protein?

Accepted Answer

A) the amino-acid sequence 
B) the amino-acid composition 
C) the location of its encoding gene within the genome 
D) the stereochemistry at the $\alpha$-carbon 
E) the sequence of tRNA molecules involved in its translation 
A) the amino-acid sequence 
B) the amino-acid composition 
C) the location of its encoding gene within the genome 
D) the stereochemistry at the $\alpha$-carbon 
E) the sequence of tRNA molecules involved in its translation

Question 5

Which of these techniques uses antibodies to detect very small amounts of specific proteins following separation by SDS-PAGE.

Accepted Answer

A) immunoblotting 
B) silverstaining 
C) Coomassie Brilliant Blue staining 
D) ELISA 
E) RIA 
A) immunoblotting 
B) silverstaining 
C) Coomassie Brilliant Blue staining 
D) ELISA 
E) RIA

Question 6

Which of these techniques is used to separate proteins mainly based on mass?

Accepted Answer

A) polyacrylamide gel electrophoresis (in the absence of SDS) 
B) SDS-PAGE 
C) isoelectric focusing 
D) immunoblotting 
E) Western blotting 
A) polyacrylamide gel electrophoresis (in the absence of SDS) 
B) SDS-PAGE 
C) isoelectric focusing 
D) immunoblotting 
E) Western blotting

Question 7

ELISA is an example of a(n):

Accepted Answer

A) enzyme assay. 
B) biological assay. 
C) binding assay. 
D) immunological assay. 
E) none of the above 
A) enzyme assay. 
B) biological assay. 
C) binding assay. 
D) immunological assay. 
E) none of the above

Question 8

Ion exchange chromatography is a commonly used method for separation of biomolecules.
a.What are the two types of ion exchange chromatography?
b.In what order would Arg,Val,and Glu elute from a carboxymethyl column at pH 6.0.Carboxymethyl is negatively charged at pH 6.0.
c.You are trying to purify a protein using ion exchange chromatography.Unfortunately,your protein remains bound to the ion exchange column or it is eluting very slowly.What are the two changes you can make,to try to elute your protein more quickly from this column?

Accepted Answer

a.cation exchange chromatography and ani

Since there are 20 standard amino acids,the number of possible linear polypeptides of length N can be expressed as:

A fast way for nature to generate new proteins is:

Which of the following has the most dramatic influence on the characteristics of an individual protein?

Which of these techniques uses antibodies to detect very small amounts of specific proteins following separation by SDS-PAGE.

Which of these techniques is used to separate proteins mainly based on mass?

ELISA is an example of a(n):

Introduction to the Chemistry of Life

Water

Nucleotides, nucleic Acids, and Genetic Information

Amino Acids

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glucose Catabolism

Glycogen Metabolism and Gluconeogenesis

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

DNA Replication, repair and Recombination

Transcription and Rna Processing

Protein Synthesis

Regulation of Gene Expression

Filters

Exam 5: Proteins: Primary Structure

Since there are 20 standard amino acids,the number of possible linear polypeptides of length N can be expressed as:

A fast way for nature to generate new proteins is:

Which of the following has the most dramatic influence on the characteristics of an individual protein?

Which of these techniques uses antibodies to detect very small amounts of specific proteins following separation by SDS-PAGE.

Which of these techniques is used to separate proteins mainly based on mass?

ELISA is an example of a(n):

Introduction to the Chemistry of Life

Water

Nucleotides, nucleic Acids, and Genetic Information

Amino Acids

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glucose Catabolism

Glycogen Metabolism and Gluconeogenesis

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

DNA Replication, repair and Recombination

Transcription and Rna Processing

Protein Synthesis

Regulation of Gene Expression

Filters