Question 1

Matching

-The absence of 2,3-BPG causes hemoglobin's affinity for oxygen to______.

Accepted Answer

A)  positively cooperative 
B)  cyanosis 
C)  His E7 
D)  decrease 
E)  R
F) hydrogen bonds
G) increase
H) symmetry
I) His F8
J) ion pairs
K) T
L) hemolytic anemia
M) sequencial 
A)  positively cooperative 
B)  cyanosis 
C)  His E7 
D)  decrease 
E)  R
F) hydrogen bonds
G) increase
H) symmetry
I) His F8
J) ion pairs
K) T
L) hemolytic anemia
M) sequencial G

Question 2

Muscle contraction is triggered

Accepted Answer

A) in response to an increase in the cytoplasmic Ca2+ concentration. 
B) in response to a decrease in the cytoplasmic Ca2+ concentration. 
C) in response to an increase in the cytoplasmic cAMP concentration. 
D) in response to a decrease in the cytoplasmic cAMP concentration. 
E) in response to an increase in the cytoplasmic titin concentration. 
A) in response to an increase in the cytoplasmic Ca2+ concentration. 
B) in response to a decrease in the cytoplasmic Ca2+ concentration. 
C) in response to an increase in the cytoplasmic cAMP concentration. 
D) in response to a decrease in the cytoplasmic cAMP concentration. 
E) in response to an increase in the cytoplasmic titin concentration. A

Question 3

______of the world's human population carries an inherited variant hemoglobin gene.

Accepted Answer

A) 5% 
B) 25% 
C) 50% 
D) 75% 
E) 90% 
A) 5% 
B) 25% 
C) 50% 
D) 75% 
E) 90% C

Question 4

Which statement about myosin is not true?

Accepted Answer

A) Myosin is a heterohexamer. 
B) Myosin contains two globular heads. 
C) Myosin contains six different polypeptides 
D) Myosin aggregates to form thick filaments 
E) All of the answers above are true of myosin. 
A) Myosin is a heterohexamer. 
B) Myosin contains two globular heads. 
C) Myosin contains six different polypeptides 
D) Myosin aggregates to form thick filaments 
E) All of the answers above are true of myosin.

Question 5

The rearrangement of T state hemoglobin to the R state

Accepted Answer

A) occurs in each protein subunit independently when its heme binds oxygen. 
B) requires the binding of at least three oxygen molecules. 
C) increases the ion pairing interactions of the C-terminal amino acids. 
D) involves the movement of the Fe(II)into the heme plane. 
E) opens a central cavity for BPG binding. 
A) occurs in each protein subunit independently when its heme binds oxygen. 
B) requires the binding of at least three oxygen molecules. 
C) increases the ion pairing interactions of the C-terminal amino acids. 
D) involves the movement of the Fe(II)into the heme plane. 
E) opens a central cavity for BPG binding.

Question 6

During the T to R conformational shift,Fe(II)drags the F-helix via a bond to the side chain of ________.

Accepted Answer

A) Leu F7 
B) Leu F4 
C) His F8 
D) Leu FG3 
E) Val FG5 
A) Leu F7 
B) Leu F4 
C) His F8 
D) Leu FG3 
E) Val FG5

Question 7

Fab fragments can be generated by

Accepted Answer

A) reduction of IgG molecules. 
B) oxidation of IgG molecules. 
C) limited digestion of IgG molecules with papain. 
D) combining two light chains. 
E) combining two heavy chains. 
A) reduction of IgG molecules. 
B) oxidation of IgG molecules. 
C) limited digestion of IgG molecules with papain. 
D) combining two light chains. 
E) combining two heavy chains.

Question 8

Which class of antibodies has been implicated in allergic reactions?

Accepted Answer

A) IgA 
B) IgD 
C) IgE 
D) IgG 
E) IgM 
A) IgA 
B) IgD 
C) IgE 
D) IgG 
E) IgM

Question 9

Myoglobin's primary physiological role is to facilitate oxygen ________.

Accepted Answer

A) storage 
B) metabolism 
C) binding 
D) reduction 
E) diffusion 
A) storage 
B) metabolism 
C) binding 
D) reduction 
E) diffusion

Question 10

The most rapid way that erythrocytes adapt to high altitudes is

Accepted Answer

A) by producing genetically altered hemoglobins that have higher O2-binding affinities. 
B) by adopting the symmetry model of allosterism. 
C) by increasing the concentration of hemoglobin. 
D) by relying upon the simpler protein myoglobin. 
E) by increasing the intracellular concentration of BPG. 
A) by producing genetically altered hemoglobins that have higher O2-binding affinities. 
B) by adopting the symmetry model of allosterism. 
C) by increasing the concentration of hemoglobin. 
D) by relying upon the simpler protein myoglobin. 
E) by increasing the intracellular concentration of BPG.

Question 11

The Ig fold can be described as a

Accepted Answer

A) globular fold composed of $\alpha$ helices. 
B) a globular fold composed of a four helix bundle. 
C) a globular fold composed of a $\beta$ sandwich. 
D) a globular fold composed of $\beta$ barrel. 
E) a coiled-coil. 
A) globular fold composed of $\alpha$ helices. 
B) a globular fold composed of a four helix bundle. 
C) a globular fold composed of a $\beta$ sandwich. 
D) a globular fold composed of $\beta$ barrel. 
E) a coiled-coil.

Question 12

Mammals and other animals have a circulatory system because diffusion is to slow to supply the tissues with oxygen in animals that are larger than 2 millimeter.Explain in one sentence why these circulatory systems contain hemoglobin or other oxygen binding proteins?

Accepted Answer

Oxygen-binding proteins increa

Question 13

You have been studying O2 binding to a hemerythrin-like protein isolated from an exotic marine worm.Your O2-binding data are shown in the table below.
a.Use the data to generate an O2-binding curve (do not forget to mark the axes).
b.Use the curve to estimate the Kd for the interaction.
c.Is there any evidence from your data that this hemoglobin-like protein binds O2 in a cooperative manner (briefly explain your answer)?

Accepted Answer

a.see curve @#IMG-DLM& b.see curve,K_d eq

Question 14

The Hill plot shows that the fourth oxygen binds to hemoglobin with a ______-fold greater affinity than the first.

Accepted Answer

A) 2 
B) 5 
C) 10 
D) 20 
E) 100 
A) 2 
B) 5 
C) 10 
D) 20 
E) 100

Question 15

Which statement about actin is not true?

Accepted Answer

A) There are two actin genes,one for F-actin and one for G-actin. 
B) Monomeric G-actin polymerizes to form F-actin. 
C) Actin filaments are polar (the ends can be distinguished) 
D) Actin can bind ATP. 
E) Actin is a common protein in nonmuscle cells. 
A) There are two actin genes,one for F-actin and one for G-actin. 
B) Monomeric G-actin polymerizes to form F-actin. 
C) Actin filaments are polar (the ends can be distinguished) 
D) Actin can bind ATP. 
E) Actin is a common protein in nonmuscle cells.

Question 16

Which of the following statements does not apply to the K value in the equation for the oxygen binding curve of myoglobin?

Accepted Answer

A) It is numerically equal to p50. 
B) It is defined as that oxygen partial pressure at which half of the oxygen binding sites are occupied. 
C) It is a measure of the affinity of myoglobin for oxygen. 
D) If Y > K,then myoglobin is less than 50% saturated with oxygen. 
E) It is the value of pO2 at which Y = 0.5. 
A) It is numerically equal to p50. 
B) It is defined as that oxygen partial pressure at which half of the oxygen binding sites are occupied. 
C) It is a measure of the affinity of myoglobin for oxygen. 
D) If Y > K,then myoglobin is less than 50% saturated with oxygen. 
E) It is the value of pO2 at which Y = 0.5.

Question 17

Matching

-Mutations that favor the oxidation of the heme iron(II)to iron(III)can cause ______.

Accepted Answer

A)  positively cooperative 
B)  cyanosis 
C)  His E7 
D)  decrease 
E)  R
F) hydrogen bonds
G) increase
H) symmetry
I) His F8
J) ion pairs
K) T
L) hemolytic anemia
M) sequencial 
A)  positively cooperative 
B)  cyanosis 
C)  His E7 
D)  decrease 
E)  R
F) hydrogen bonds
G) increase
H) symmetry
I) His F8
J) ion pairs
K) T
L) hemolytic anemia
M) sequencial

Question 18

The energy needed to drive muscle contraction comes from ATP hydrolysis that is carried out by

Accepted Answer

A) G-actin. 
B) F-actin. 
C) myosin heads. 
D) myosin tails. 
E) tropomyosin. 
A) G-actin. 
B) F-actin. 
C) myosin heads. 
D) myosin tails. 
E) tropomyosin.

Question 19

When the partial pressure of O2 in venous blood is 30 torr,the saturation of myoglobin with O2 is ______ while the saturation of hemoglobin with O2 is ______.

Accepted Answer

A) 0)55,0.91 
B) 0)91,0.55 
C) 2)8 torr,26 torr 
D) 0)91,0.97 
E) none of the above 
A) 0)55,0.91 
B) 0)91,0.55 
C) 2)8 torr,26 torr 
D) 0)91,0.97 
E) none of the above

Question 20

Myoglobin is an oxygen binding protein in muscle.Describe in one sentence the overall structure of myoglobin.

Accepted Answer

Myoglobin is a globular protei

Exam 7: Protein Function Part I: Myoglobin and Hemoglobin

Matching -The absence of 2,3-BPG causes hemoglobin's affinity for oxygen to______.

Muscle contraction is triggered

______of the world's human population carries an inherited variant hemoglobin gene.

Which statement about myosin is not true?

The rearrangement of T state hemoglobin to the R state

During the T to R conformational shift,Fe(II)drags the F-helix via a bond to the side chain of ________.

Fab fragments can be generated by

Which class of antibodies has been implicated in allergic reactions?

Myoglobin's primary physiological role is to facilitate oxygen ________.

The most rapid way that erythrocytes adapt to high altitudes is

The Ig fold can be described as a

Mammals and other animals have a circulatory system because diffusion is to slow to supply the tissues with oxygen in animals that are larger than 2 millimeter.Explain in one sentence why these circulatory systems contain hemoglobin or other oxygen binding proteins?

The Hill plot shows that the fourth oxygen binds to hemoglobin with a ______-fold greater affinity than the first.

Which statement about actin is not true?

Which of the following statements does not apply to the K value in the equation for the oxygen binding curve of myoglobin?

Matching -Mutations that favor the oxidation of the heme iron(II)to iron(III)can cause ______.

The energy needed to drive muscle contraction comes from ATP hydrolysis that is carried out by

When the partial pressure of O2 in venous blood is 30 torr,the saturation of myoglobin with O2 is ______ while the saturation of hemoglobin with O2 is ______.

Myoglobin is an oxygen binding protein in muscle.Describe in one sentence the overall structure of myoglobin.

Introduction to the Chemistry of Life

Water

Nucleotides, nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glucose Catabolism

Glycogen Metabolism and Gluconeogenesis

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

DNA Replication, repair and Recombination

Transcription and Rna Processing

Protein Synthesis

Regulation of Gene Expression

Filters

When the partial pressure of O₂ in venous blood is 30 torr,the saturation of myoglobin with O₂ is while the saturation of hemoglobin with O₂ is .