Question 1

Matching
Choose the correct answer from the list.Not all the answers will be used.

-E.coli primer synthesis is catalyzed by a ______.

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 2

In most organisms,replication proceeds in a ______ manner from the ______.

Accepted Answer

A) discontinuous;leading strand 
B) unidirectional;chromosome ends 
C) bidirectional;replication origin 
D) bidirectional;chromosome ends 
E) discontinuous;chromosome ends 
A) discontinuous;leading strand 
B) unidirectional;chromosome ends 
C) bidirectional;replication origin 
D) bidirectional;chromosome ends 
E) discontinuous;chromosome ends

Question 3

Defects in the proteins ______,which associate with RAD-51,are correlated with several types of cancer.

Accepted Answer

A) BRCA1 and BRCA2 
B) RecU and RecB 
C) Pol $\alpha$ and Pol $\beta$ 
D) DSB and NHEJ 
E) RecA and RecUGA 
A) BRCA1 and BRCA2 
B) RecU and RecB 
C) Pol $\alpha$ and Pol $\beta$ 
D) DSB and NHEJ 
E) RecA and RecUGA

Question 4

Matching
Choose the correct answer from the list.Not all the answers will be used.

-Direct reversal of pyrimidine dimers in prokaryotic cells is catalyzed by a DNA _____.

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 5

When a section of DNA is damaged and polymerization cannot proceed,_____ repair can reconstitute the damaged replication fork.

Accepted Answer

A) mismatch 
B) nucleotide excision 
C) recombination 
D) photoreactivation 
E) base excision 
A) mismatch 
B) nucleotide excision 
C) recombination 
D) photoreactivation 
E) base excision

Question 6

The experiments of Reiji Okazaki allowed determination that DNA synthesis occurs in
(I)a continuous fashion.
(II)different ways on the lagging strand than the leading strand.
(III)resulted in new synthesized DNA fragments containing the radioactive isotope.

Accepted Answer

A) I,II,III 
B) II,III 
C) I,II 
D) I,III 
E) II only 
A) I,II,III 
B) II,III 
C) I,II 
D) I,III 
E) II only

Question 7

A new DNA polymerase exhibits: (1)high processivity, (2)low fidelity,and (3)ability to replace RNA with DNA.Which of the following is probably TRUE?

Accepted Answer

A) It has 3' exonuclease activity. 
B) It has 5'exonuclease activity. 
C) It has RNA primase activity. 
D) It is not associated with a clamp. 
E) both A and B 
A) It has 3' exonuclease activity. 
B) It has 5'exonuclease activity. 
C) It has RNA primase activity. 
D) It is not associated with a clamp. 
E) both A and B

Question 8

E.coli DNA repair enzymes act on the sequence.Which of the following will result?

Accepted Answer

A) The underlined A will be changed to a C. 
B) The underlined G will be changed to a T. 
C) The underlined A will be methylated. 
D) The underlined G will be methylated. 
E) More than one of the above is correct 
A) The underlined A will be changed to a C. 
B) The underlined G will be changed to a T. 
C) The underlined A will be methylated. 
D) The underlined G will be methylated. 
E) More than one of the above is correct

Question 9

Matching
Choose the correct answer from the list.Not all the answers will be used.

-Genes can be moved by _______.

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 10

Matching
Choose the correct answer from the list.Not all the answers will be used.

-In yeast,DNA replication begins at sequences known as ______.

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 11

Which of the following statements about telomerase is FALSE?
(I)Telomerase is an RNA polymerase.
(II)Telomerase has a 5'->3' polymerase activity.
(III)Telomerase uses RNA as a template.
(IV)The product of the telomerase-catalyzed reaction is rich in G and T.

Accepted Answer

A) I only 
B) II only 
C) II,III 
D) I,III 
E) II,IV 
A) I only 
B) II only 
C) II,III 
D) I,III 
E) II,IV

Question 12

Pol I is proteolytically cleaved to yield the ____,which lacks 5'$\to$3' exonuclease activity.

Accepted Answer

A) Klenow fragment 
B) Klentaq1 polymerase 
C) Okazaki fragment 
D) ligase 
E) primase 
A) Klenow fragment 
B) Klentaq1 polymerase 
C) Okazaki fragment 
D) ligase 
E) primase

Question 13

Which of the following would result in single stranded DNA?

Accepted Answer

A) Klenow fragment 
B) Klentaq I 
C) DnaB 
D) DnaG 
E) pol III 
A) Klenow fragment 
B) Klentaq I 
C) DnaB 
D) DnaG 
E) pol III

Question 14

Matching
Choose the correct answer from the list.Not all the answers will be used.

-G-rich polynucleotides can form _______

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 15

Which of the following statement(s)about DNA polymerase III (Pol III)is (are)TRUE?
(I)DNA polymerase III has greater accuracy than RNA polymerase.
(II)Pol III is the primary replicase of E.coli.
(III)Pol III does not contain 5' to 3' exonuclease activity.

Accepted Answer

A) I,II. 
B) II,III. 
C) III only. 
D) I only. 
E) I,II,III. 
A) I,II. 
B) II,III. 
C) III only. 
D) I only. 
E) I,II,III.

Question 16

Matching
Choose the correct answer from the list.Not all the answers will be used.

-Termination of replication fork movement in E.coli involves the binding of the ______ protein at a Ter site.

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 17

Matching
Choose the correct answer from the list.Not all the answers will be used.

-DNA separated at the replication fork is stabilized by ______ .

Accepted Answer

A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive 
A)  SSB 
B)  Tus 
C)  primosome 
D)  ARS 
E)  ATS
F) primer
G) transposons
H) nick translation
I) photolyase
J) processive
K) G-loops
L) G-quartets
M) accurate
N) lagging strand synthesis
O) MCM
P) abortive

Question 18

Bacteria have obtained the $\beta$-lactamase gene which encodes a protein that renders all $\beta$-lactem antibodies such as ampicillin and penicillin inactive.Which of the following would result in an increased number of bacteria with this ability?
(I)a transposon with the $\beta$-lactamase gene
(II)a recombination event within the original strain that results in a point mutation inactivating $\beta$-lactamase
(III)an insertion in the original strain rendering transposase inactive
(IV)a deletion in the insertion sequence of the transposon

Accepted Answer

A) I,II,III,IV 
B) I only 
C) II only 
D) II,III 
E) III,IV 
A) I,II,III,IV 
B) I only 
C) II only 
D) II,III 
E) III,IV

Question 19

Which of the following are TRUE about transposons?
(I)They are normal components of plasmids.
(II)They often carry genes for antibiotic resistance.
(III)They insert at a target sequence that is a repetitive unit of about 200 bp.

Accepted Answer

A) I,II,III 
B) I,II 
C) II,III 
D) II only 
E) III only 
A) I,II,III 
B) I,II 
C) II,III 
D) II only 
E) III only

Question 20

Replication of circular DNA occurs at the "bubbles" or "eyes",also called ______ .

Accepted Answer

A) "$\theta$ structures" 
B) "$\phi$ bubbles" 
C) "$\theta$ bubbles" 
D) "$\sigma$ structures" 
E) "$\sigma$ bubbles" 
A) "$\theta$ structures" 
B) "$\phi$ bubbles" 
C) "$\theta$ bubbles" 
D) "$\sigma$ structures" 
E) "$\sigma$ bubbles"

Exam 25: DNA Replication, repair and Recombination

Matching Choose the correct answer from the list.Not all the answers will be used. -E.coli primer synthesis is catalyzed by a ______.

In most organisms,replication proceeds in a ______ manner from the ______.

Defects in the proteins ______,which associate with RAD-51,are correlated with several types of cancer.

Matching Choose the correct answer from the list.Not all the answers will be used. -Direct reversal of pyrimidine dimers in prokaryotic cells is catalyzed by a DNA _____.

When a section of DNA is damaged and polymerization cannot proceed,_____ repair can reconstitute the damaged replication fork.

The experiments of Reiji Okazaki allowed determination that DNA synthesis occurs in (I)a continuous fashion. (II)different ways on the lagging strand than the leading strand. (III)resulted in new synthesized DNA fragments containing the radioactive isotope.

A new DNA polymerase exhibits: (1)high processivity, (2)low fidelity,and (3)ability to replace RNA with DNA.Which of the following is probably TRUE?

E.coli DNA repair enzymes act on the sequence.Which of the following will result?

Matching Choose the correct answer from the list.Not all the answers will be used. -Genes can be moved by _______.

Matching Choose the correct answer from the list.Not all the answers will be used. -In yeast,DNA replication begins at sequences known as ______.

Which of the following statements about telomerase is FALSE? (I)Telomerase is an RNA polymerase. (II)Telomerase has a 5'->3' polymerase activity. (III)Telomerase uses RNA as a template. (IV)The product of the telomerase-catalyzed reaction is rich in G and T.

Pol I is proteolytically cleaved to yield the ____,which lacks 5' →\to→ 3' exonuclease activity.

Which of the following would result in single stranded DNA?

Matching Choose the correct answer from the list.Not all the answers will be used. -G-rich polynucleotides can form _______

Which of the following statement(s)about DNA polymerase III (Pol III)is (are)TRUE? (I)DNA polymerase III has greater accuracy than RNA polymerase. (II)Pol III is the primary replicase of E.coli. (III)Pol III does not contain 5' to 3' exonuclease activity.

Matching Choose the correct answer from the list.Not all the answers will be used. -Termination of replication fork movement in E.coli involves the binding of the ______ protein at a Ter site.

Matching Choose the correct answer from the list.Not all the answers will be used. -DNA separated at the replication fork is stabilized by ______ .

Which of the following are TRUE about transposons? (I)They are normal components of plasmids. (II)They often carry genes for antibiotic resistance. (III)They insert at a target sequence that is a repetitive unit of about 200 bp.

Replication of circular DNA occurs at the "bubbles" or "eyes",also called ______ .

Introduction to the Chemistry of Life

Water

Nucleotides, nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glucose Catabolism

Glycogen Metabolism and Gluconeogenesis

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Transcription and Rna Processing

Protein Synthesis

Regulation of Gene Expression

Filters

In most organisms,replication proceeds in a manner from the .

Pol I is proteolytically cleaved to yield the ____,which lacks 5' $\to$ 3' exonuclease activity.